高分子对酶,抗体DNA的修饰,固定化及其生物医学应用

为发展适于生物医用的生物功能高分子材料，本实验室近年来研究了可溶性高分子对Ｌ－天冬酰胺酶的修饰，纳米磁性高分子微粒对酶或抗体的固定化，亚微米高分子微球固定化碱性磷酸酶及其在ＤＮＡ检测中的应用，高分子微球固定化酶的合成与性能，酶在导电高分子膜上的固定化及生物传感器制备等，本文对此进行简要总结。     

磁性高分子微球的制备和应用研究进展

磁性高分子微球是近２０年来研究的一类新型功能材料，在生物医学，细胞学和生物工程等领域有着广泛的应用前景，本文对磁性高分子微球的性质，制备方法及功能经方法作了详细评述，介绍了磁性高分子微球在细胞分离，固定化酶，免疫测定，生物导弹，ＤＮＡ分离及核酸杂交等领域的应用。     

超顺磁性高分子微球的制备与表征

用化学共沉淀方法制备了Fe3O4纳米微粒,并用油酸(十八烯酸)和十二烷基苯磺酸钠为双层表面活性剂进行表面修饰,制备了稳定的水分散性纳米Fe3O4可聚合磁流体.在Fe3O4磁流体存在下,将苯乙烯与甲基丙烯酸通过乳液聚合方法制备了磁性高分子微球.透射电镜研究表明,Fe3O4微粒的平均粒径在10nm左右,乳液聚合形成的磁性高分子微球的粒径平均约为130nm;用超导量子干涉仪对微粒及高分子微球进行了磁性表征,结果表明,合成的Fe3O4纳米微粒以及磁性高分子微球均具有超顺磁性.同时,还用红外光谱及X射线衍射表征了磁性高分子微球的化学成分和晶体结构.用热失重方法测得磁性高分子微球中磁性物质的含量为23.6%.     

聚合物基磁性复合微球的研究近况

磁性聚合物微球作为一种新兴起的复合功能材料,由于具有纳米尺寸、超顺磁性、良好的生物相容性、低毒性诸多优点,受到了人们广泛而深入的研究。本文简要介绍了无机磁性粒子的制备方法和磁性聚合物微球的分类,主要对磁性高分子微球的传统制备方法和涌现新方法新技术进行了阐述,并分析了各种制备磁性聚合物微球方法的优缺点及主要影响因素。除此之外,本文也介绍了磁性聚合物微球近年来最新研究成果及应用领域,并对高分子微球未来发展趋势和存在的问题进行了分析。     

功能化磁性高分子复合微球的研究进展

功能化磁性高分子复合微球作为一种新型功能材料,在许多领域具有广阔的应用前景。本文对近年来功能化磁性高分子复合微球的制备方法进行了总结,对当前不同方法的优缺点进行了评价与分析;对功能化磁性高分子复合微球在生物工程领域,医学领域,环境、食品领域和功能材料领域进行了阐述。并综合分析了磁性微球在各个领域的优势及已经取得的成果。最后,展望了功能化磁性高分子复合微球的发展前景,提出自己的观点,并列出亟待解决的四个问题。     

磁性高分子微球用于固定化酶的研究进展生物材料结构及性质的基础研究及其应用于药物的控制释放

磁性高分子微球是近20年来发展起来的一种新型功能高分子材料,并已在生物化工、细胞学、生物医学工程等领域得到了广泛应用。本文主要介绍磁性高分子微球的制备和性质以及在固定化酶中的应用。     

磁性高分子微球用于固定化酶的研究进展

磁性高分子微球是近20年来发展起来的一种新型功能高分子材料,并已在生物化工、细胞学、生物医学工程等领域得到了广泛应用.本文主要介绍磁性高分子微球的制备和性质以及在固定化酶中的应用.     

磁性高分子微球用于固定化酶的研究进展：生物材料结构及？…

磁性高分子微球是近２０年来发展起来的一种新型功能高分子材料,并已在生物化工、细胞学、生物医学工程等领域得到了广泛应用。本文主要介绍磁性高分子微球的制备和性质以及在固定化酶中的应用。     

Fe_3O_4／P（St－AA）核－壳复合微球的制备和表征

以磁性氧化铁胶体粒子为种子，运用分散聚合法，制备出具有磁响应性的Ｆｅ_３Ｏ_４／Ｐ（Ｓｔ－ＡＡ）核－壳复合微球。考察了复合微球的形态及结构，测定了复合微球的粒径和磁响应性，研究了分散介质、引发剂、聚合单体和种子粒子等因素对复合微球形成的影响。     

分散聚合制备聚苯乙烯/聚氧乙烯两亲磁性高分子微球

采用分散聚合法，以乙醇／水为介质，在Fe3O4磁流体存在下，通过苯乙烯与聚氧乙烯大分子单体共聚制备休磁性高分子微球。 研究了聚合条件对微球粒径的影响。通过改变聚合条件，可以得到平均粒径为5μm-100μm的两亲磁性高分子微球。     

α-Amino acid derived enaminones and their application in the synthesis of N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates and other heterocycles

A new and simple synthesis of novel N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates, which exist in equilibrium with their 4-oxo tautomers, has been developed in two steps starting from N-protected α-amino acids. The key intermediates are enaminones, which can also be isolated, characterized, and used for the construction of other functionalized heterocycles, before they spontaneously decompose to pyrrole products. 4-Hydroxypyrroles are prone to partial aerial oxidation but can be efficiently alkylated or reduced to stable polysubstituted pyrrolidine derivatives.     

Specific intramolecular aromatic CH insertion of diazosulfonamides

The chemoselectivity in the intramolecular CH insertion of various diazosulfonamides has been experimentally studied. The results reveal that the aliphatic 1,4-, 1,5-, or 1,6-C(sp³)?H insertions of diazosulfonamides are not accessible, while the aromatic 1,5-C(sp²)?H insertion can be realized specifically by adjusting the diazo-adjacent group. In addition, the general chemoselectivities in the intramolecular CH insertions of diazosulfonyl compounds are summarized. Generally, diazosulfones undergo both aromatic 1,5-C(sp²)?H and aliphatic 1,5- and 1,6-C(sp³)?H insertions, while diazosulfonates undergo aliphatic 1,5- and 1,6-C(sp³)?H insertions. However, diazosulfonamides only undergo aromatic 1,5-C(sp²)?H insertion.     

N-Heterocyclic carbene-palladacyclic complexes: synthesis,characterization and their applications in the C-N coupling and α-arylation of ketones using aryl chlorides

N-Heterocyclic carbene-palladacyclic complexes 3 were successfully achieved in a one-pot procedure under mild conditions. The structure of 3a was unambiguously confirmed by X-ray single crystal diffraction and it was an active catalyst in the Buchwald-Hartwig amination and α-arylation of ketones even at very low catalyst loadings (0.01?mol%).     

Iodine-mediated oxidative Pictet-Spengler reaction using terminal alkyne as the 2-oxoaldehyde surrogate for the synthesis of 1-aroyl-β-carbolines and fused-nitrogen heterocycles

An efficient iodine-mediated oxidative Pictet-Spengler reaction in dimethyl sulphoxide (DMSO) using terminal alkynes as the 2-oxoaldehyde surrogate for the synthesis of aryl (9H-pyrido[3,4-b]indol-1-yl)methanones is described. The scope of the protocol includes the total synthesis of Fascaplysin, Eudistomins Y₁ and Y₂. The methodology is extended for preparing pyrrolo[1,2-a]-quinoxaline and indolo[1,5-a]quinoxaline derivatives. The utility of 1-aroyl-β-carbolines was demonstrated by performing palladium-catalyzed β-carboline directed ortho-C(sp2)-H functionalization of the phenyl ring with thiomethyl (SMe) group using DMSO as source and for accessing 4-aryl-canthin-6-ones.     

Facile synthesis of bicyclic 1-arylpyrazol-5-ones

In this Letter, we described a facile method for constructing fused bicyclic 1-arylpyrazol-5-one ring system. We employed various methylene-containing carboxylic acids as the substrates and proved that the pyrazolone ring closure requires activated methylene group in intermediate II. Accordingly, a series of structurally diversified, fused bicyclic 1-arylpyrazol-5-ones was prepared in moderate to high yields using the requisite substrates.     

A carbanion induced synthesis of highly congested pyrazole and imidazole containing heterocycles

An efficient approach to the synthesis of highly congested di, penta and hexacyclic pyrazoles as well as imidazole fragment containing novel heterocyclic molecule has been developed through a carbanion induced transformation of suitably functionalized 2H-pyran-2-ones, benzo[h]chromene and thiochromeno[4,3-b]pyrans. Due to the presence of fluorescence, we report their prime application metal sensor as off/on switching in ferric ions.     

Synthesis of pyrrolo[1,2-a]pyrazines and pyrazino[1,2-a]indoles by Curtius reaction in Morita-Baylis-Hillman derivatives

Synthesis of substituted pyrrolo[1,2-a]pyrazines and pyrazino[1,2-a]indoles from the Morita-Baylis-Hillman derivatives of acrylates via saponification followed by Curtius reaction is described.     

正丁胺对激光染料荧光谱影响的研究

用正丁胺作为碳源,采用射频辉光放电制备碳膜,选用激光染料R6G和聚乙二醇混合液作为蒸气源,采用单源热蒸发,在蒸发室与染料同时沉积得到混合膜,用拉曼光谱和红外光谱分析了碳膜的结构和键合方式,分析表明:碳膜中存在胺基团和氢原子.混合膜的荧光谱测量结果表明,认为正丁胺对染料荧光谱的影响是因为胺基和氢原子的存在.     

Selective synthesis of 4,5-dihydroimidazo- and imidazo[1,5-a]quinoxalines via modified Pictet–Spengler reaction

An efficient tandem approach for the selective synthesis of 4,5-dihydroimidazo[1,5-a]quinoxalines 6a–g and imidazo[1,5-a]quinoxalines 7a–h by the reaction of 2-imidazolyl anilines 4a–c with aryl aldehydes 5a–k under mild reaction conditions is described. Introduction of electron releasing alkyl groups in substrates 4a–b was found to be instrumental for the success of the reaction.     

Understanding the Diels-Alder reactivity of 1,2-azaborine analogues

The Diels-Alder reactivity of 1,2-heteroborines (H₄C₄B(H)X, X?=?NH, PH, AsH; O, S, Se) has been computationally explored by means of Density Functional Theory (DFT) calculations. The influence of the HB?=?X fragment on the reactivity of the system has been quantitatively analyzed in detail by means of the so-called Activation Strain Model (ASM) of reactivity. It is found that the interaction between these species and the dienophile is significantly stronger than that computed for their all-carbon isoelectronic counterpart, benzene. In addition, the strain energy plays a key role in the observed reactivity trends. The role of the aromaticity strength of these heteroarenes on the reactivity is also assessed.