新型胺基壳聚糖树脂的合成及其对胆红素吸附性能的研究

以壳聚糖为原料，经羟丙基氯化、胺基化，制备了一种新型胺基壳聚糖树脂，研究了该树脂对胆红素的吸附性能。结果表明：胺基壳聚糖树脂的胺基含量比壳聚糖高，该树脂对游离胆红素的吸附在3h基本达到平衡，吸附量随着胺基含量增加而增加，树脂的吸附性能受温度、离子强度影响。胺基壳聚糖树脂对血清白蛋白结合胆红素的吸附量大于壳聚糖，但低于对游离胆红素的吸附。     

3种树脂对水溶液中单宁吸附性能的比较研究

通过静态吸附实验,研究了大孔吸附树脂NG-8、胺基化大孔吸附树脂NG-9和胺基化超高交联大孔吸附树脂NDA-99对水溶液中单宁的吸附行为特性.结果表明,3种树脂对单宁的吸附均符合Langmuir等温吸附方程,树脂对单宁的吸附容量NG-9＞NG-8＞NDA-99;吸附过程符合准二级动力学方程,吸附速率常数NG-8＞NG-9＞NDA-99.树脂的孔结构与表面化学性质是影响树脂吸附性能的重要因素.     

改性壳聚糖树脂对利尿剂的吸附性能

用琥珀酸酐、苯甲酸酐、聚乙烯亚胺和 3 氯 2 羟丙基三甲基氯化铵对壳聚糖进行改性 ,分别在其氨基上引入羧基、苯环、多氨基和季胺基 ,并利用红外谱图对于改性后的壳聚糖的结构进行了分析 .应用相转移法制备了 4种改性壳聚糖的微球 ,实验研究了这 4种微球对 9种不同利尿剂的吸附性能 .结果表明 ,由于Lewis酸碱相互作用 ,引入羧基后的壳聚糖树脂对 3种碱性利尿剂的吸附量有了 15 %～ 36 %的提高 ,而引入多氨基的壳聚糖树脂对四种酸性利尿剂的吸附量分别提高了 4 8 5 %～ 2 0 9% ;由于苯环和利尿剂的憎水性基团的相互作用 ,引入苯环后的壳聚糖树脂对所有的利尿剂的吸附量都有所提高 ,其幅度为 15 %～ 6 1% ;因为季胺基团和羧基之间发生的离子交换作用 ,引入季胺基后的树脂对具有羧基的利尿剂吸附量有显著的提高 ,尤其对布美它尼的吸附量提高了 2倍以上 .     

壳聚糖-沸石复合膜对茶汤中茶多酚的选择性吸附

将壳聚糖和沸石物理共混制成膜,以壳聚糖-沸石复合膜作为吸附剂,对茶汤中茶多酚进行吸附研究,考察了吸附剂用量、时间、温度、浓度、pH值等因素对吸附效果的影响,就壳聚糖-沸石复合膜对茶多酚的吸附动力学进行初步探讨,并用HPLC分析其对茶多酚和咖啡因的选择性吸附。结果表明,将4.0m L 4%壳聚糖和0.2%沸石的混合液制成的壳聚糖-沸石复合膜、25.00m L 1.049mg/mL原茶汤在吸附时间2h、温度30℃的条件下吸附,吸附率可达76.3%,吸附量为171.2mg/g;HPLC谱图表明,壳聚糖-沸石膜对EGCG具有很好的选择吸附性能。     

大孔吸附树脂对湖南黑茶茶多酚的吸附热力学和动力学研究

以湖南黑茶为原料,采用超声法提取湖南黑茶中茶多酚,后通过静态吸附和解吸实验,对5种大孔吸附树脂进行筛选,研究大孔吸附树脂对湖南黑茶茶多酚的吸附热力学和动力学。结果表明,大孔吸附树脂D-101对湖南黑茶茶多酚有较大的吸附量、较强的解吸能力。大孔吸附树脂D-101对黑茶茶多酚的吸附符合Freundlich等温吸附方程;且ΔH0、ΔG0、ΔS0,表明黑茶茶多酚在大孔吸附树脂D-101上的吸附属于可自发进行的物理吸附,吸附过程为放热、熵减过程。吸附动力学研究结果表明,准二级动力学方程的计算值与实验值吻合较好,适合描述大孔吸附树脂D-101对黑茶茶多酚的吸附过程。     

胺基化壳聚糖微球上染料的吸附平衡和动力学

采用胺基化壳聚糖微球作为吸附剂,对三种阴离子偶氮染料——甲基橙MO[MethylOrange],活性艳红RR2[ReactiveRed2]和直接黑DB19[DirectBlack19]进行吸附,对此过程的吸附平衡和吸附动力学进行了研究。结果表明,三种染料的吸附体系是以物理吸附为主,兼有化学吸附,属多层吸附;二级速率方程描述三种染料在胺基化壳聚糖微球上的吸附行为比较贴切。     

氨基酸修饰壳聚糖对胆固醇的吸附作用

通过将不同脱乙酰度的壳聚糖粉末与戊二醛交联,再经苯丙氨酸和色氨酸修饰,得到了两种珠状壳聚糖吸附剂,并进而研究了有关吸附剂对胆因醇的吸附性能。实验表明,交联壳聚糖珠对ＣＨＯ的吸附能力比壳聚糖粉末降低,而经不同氨基酸修饰后的壳聚糖珠对ＣＨＯ吸附能力提高,用Ｐｈｅ修饰比用Ｔｒｙ修饰的珠吸附性能更好些。     

3种树脂对水溶液中对氯苯胺吸附性能的比较研究

通过静态吸附实验,研究了超高交联吸附树脂NDA-100、胺基化超高交联吸附树脂AH-1和AH-3对水溶液中对氯苯胺的吸附行为特性。结果表明,3种树脂吸附对氯苯胺均符合Langmuir等温吸附方程,吸附容量AH-1NDA-100AH-3。树脂的孔结构与表面化学性质是影响树脂吸附性能的重要因素。对氯苯胺在3种树脂上的吸附容量随溶液中氯化钠含量和pH值的升高而升高。     

胺修饰酚醛树脂对酸性橙Ⅱ的吸附性能研究

研究了自制胺修饰酚醛树脂(AMPF)对水溶性染料酸性橙II的吸附性能,探讨了pH值、温度、浓度等因素对酸性橙吸附性能的影响。结果表明,AMPF与市售大孔树脂D296和D301相似,对酸性橙均具有较强的吸附能力。温度升高有利于AMPF对酸性橙的吸附;酸性橙浓度升高,吸附量增大。AMPF对酸性橙的吸附符合Boyd液膜扩散控制。各种树脂对酸性橙的平衡吸附数据符合Langmuir方程和Freundlich等温式,其相关系数大于0.98,n均大于1,表明为优惠吸附。     

壳聚糖及其改性衍生物对锆(Ⅳ)离子的吸附研究

壳聚糖经双氧水降解得到低分子量壳聚糖,用水杨醛对低分子量壳聚糖进行化学改性得到其相应的衍生物.分别研究了壳聚糖和水杨醛改性的低分子量壳聚糖对锆(Ⅳ)离子的吸附,考察了锆(Ⅳ)离子起始浓度、溶液pH值、吸附时间以及温度对这两种吸附剂吸附锆(Ⅳ)离子性能的影响,得出了最佳吸附条件.用红外对最佳条件下的吸附产物进行了表征.结果表明,壳聚糖吸附锆离子的最佳条件为:锆(Ⅳ)离子的起始浓度为6.5×10-4g/mL,振摇时间为6.5h,pH值为5～6;水杨醛改性的低分子量壳聚糖吸附锆离子的最佳条件为:锆离子的起始浓度为4.7×10-4g/mL,振摇时间为5h,pH值为5～6.这两种吸附剂对锆(Ⅳ)离子的吸附受温度影响均不大,吸附行为均满足Langmuir等温式.红外光谱分析表明,锆(Ⅳ)离子与这两种吸附剂均发生了配位作用.     

染料壳聚糖微球的制备及其对牛血清白蛋白(BSA)吸附性能的研究

采用反相悬浮交联法制备壳聚糖微球,对微球进行羟丙基氯化及氨基化,并偶联色素配体Cibacron Blue F3GA,得到一种新型染料亲和吸附剂.以牛血清白蛋白(BSA)为目标蛋白,考察了该染料亲和吸附剂的吸附性能,发现其对BSA有较高的吸附量(95.2mg/g),吸附行为满足Langmuir吸附等温式.负载牛血清白蛋白的微球容易洗脱,洗脱率高达99％.     

Surface and biological activity of sophorolipid molecules produced by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576

The ion-imprinted magnetic chitosan resins (IMCR) prepared using U(VI) as a template and glutaraldehyde as a cross-linker showed higher adsorption capacity and selectivity for the U(VI) ions compared with the non-imprinted magnetic chitosan resins (NIMCR) without a template. The results showed that the adsorption of U(VI) on the magnetic chitosan resins was affected by the initial pH value, the initial U(VI) concentration, as well as the temperature. Both kinetics and thermodynamic parameters of the adsorption process were estimated. These data indicated an exothermic spontaneous adsorption process that kinetically followed the second-order adsorption process. Equilibrium experiments were fitted in Langmuir, Freundlich, and Dubinin-Radushkevich adsorption isotherm models to show very good fits with the Langmuir isotherm equation for the monolayer adsorption process. The monolayer adsorption capacity values of 187.26 mg/g for IMCR and 160.77 mg/g for NIMCR were very close to the maximum capacity values obtained at pH 5.0, temperature 298 K, adsorbent dose 50 mg, and contact time 3 h. The selectivity coefficient of uranyl ions and other metal ions on IMCR indicated an overall preference for uranyl ions. Furthermore, the IMCR could be regenerated through the desorption of the U(VI) ions using 0.5 M HNO(3) solution and could be reused to adsorb again.     

胺基修饰的高度交联聚苯乙烯树脂对间苯二酚吸附行为研究

研究了AH系列胺基修饰的超高交联树脂对水溶液中间苯二酚的静态吸附行为特征,结果表明,它们对间苯二酚的吸附容量明显高于母体交联树脂NDA-100和大孔弱碱性阴离子交换树脂D301.AH系列树脂与吸附质分子之间不仅有范德华作用力,还存在着氢键等作用力.该类树脂对间苯二酚的吸附为自发的放热过程,属于以物理作用为主兼有弱化学作用的吸附过程.吸附速率符合准一级动力学方程,表观吸附速率常数随树脂胺基含量的升高而降低.     

染料壳聚糖微球的制备及其对人血清白蛋白吸附性能研究

人血清白蛋白(Human Serum Albumin,HSA)是血浆中含量最丰富的蛋白质,约占血浆总蛋白的60%.在人体内,HSA有许多重要的生理功能[1],临床上广泛应用于手术输血和危重病人补液,治疗创伤休克、烧伤、水肿和低白蛋白血症等,而且能增强人体抵抗能力,是迄今为止产量最大、临床用量最大     

THE PREPARATION OF CROSSLINKED N，O—CARBOXYMETHYL CHITOSAN RESINS AND THEIR ADSORPTION PROPERTIES FOR TRIGLYCERIDE IN SERUM

Crosslinked N,O-carboxymethyl chitosan resins which can selectively adsorb triglyceride,were prepared by the reaction of N,O-carboxymethyl chitosan with glutaraldehyde solution.Adsorption experiments were performed by adding the resins to the serum.The results showed that this type of adsoirbent could cut down the concentration of triglyceride in serum by 56.4%(3.35mg/g resin)at most,while concentration of the total protein(TP) decreased only by 10.9% at least,so this novel adsorbent can be used to cure hypertriglyceridemia by hemoperfusion in the future.     

Adsorption properties of tea polyphenols onto three polymeric adsorbents with amide group

Macroporous crosslinked poly(N-methyl-N-p-vinylbenzylacetamide) (PMVBA), poly(N-methyl-N-p-vinylbenzylurea) (PMVBU), and poly(N-methylacrylamide) (PMA) were prepared and their adsorption behaviors for tea polyphenols (TP) were investigated in aqueous solution. The results indicated that their adsorption capacities for TP followed an order as: PMVBU>PMVBA>PMA. The adsorption isotherms were fitted to Freundlich isotherm and adsorption enthalpies were calculated. It was found that Freundlich isotherm was suitable to characterize the adsorption process and their adsorption enthalpies for TP gave the same order as: PMVBU>PMVBA>PMA. Surface energy heterogeneity was analyzed by Do's model and the surface of PMVBU was shown to be the most homogeneous. Analysis of adsorption mechanism suggested that multiple hydrogen bonding, hydrophobic interaction, and pi-pi stacking were responsible for the adsorption of TP onto the adsorbents.     

THE PREPARATION OF CROSSLINKED N,O-CARBOXYMETHYL CHITOSAN RESINSAND THEIR ADSORPTION PROPERTIESFOR TRIGLYCERIDE IN SERUM

INTRODUCTIONItiswidelyknownthattheplasmalevelsoftriglyceride(TG)--richlipoproteinsarerelatedtotheriskofcoronaryarterydisease(CAD).Thehighlevelsof.ThisprojectwassupportedbyNationalScienceFoundationofP.R.ChinaTGcancausehypertriglyceridemia,oneoftheriskfactorsforthedevelopmentof.th.....l...,i.[1--3J.Somepatientssufferingfromseverehypertriglyceride.Inthesecases,attemptshavebeenmadetowardsdirectremovalofserumtriglyceridebyplasmaexchang,doublefiltration,andTGadsorption.Amongthose,selecti…     

Kinetics and thermodynamics of the adsorption of some dyestuffs and p-nitrophenol by chitosan and MCM-chitosan from aqueous solution

The effect of initial concentration, temperature, and shaking rate on the adsorption of three dyestuffs [orange II (O-II), crystal violet (CV), and reactive blue 5 (RB5)] and an ideal adsorbate, p-nitrophenol (PNP), by chitosan (Sigma C-3646) and the effect of temperature on the adsorption of O-II and CV by monocarboxymethylated chitosan (MCM-chitosan) were investigated. Kinetic data obtained for the adsorption of each dyestuff and PNP by chitosan and of O-II and CV by MCM-chitosan at different temperatures were applied to the Lagergren equation, and adsorption rate constants (k(ads)) at these temperatures were determined. These rate constants related to the adsorption of O-II and RB5 by chitosan and of O-II by MCM-chitosan were applied to the Arrhenius equation, and activation energies (E(a)) were determined. In addition, the isotherms for adsorption, at different temperatures, of each dyestuff and PNP by chitosan and of O-II and CV by MCM-chitosan were also determined. These isothermal data were applied to linear forms of isotherm equations that they fit, and isotherm constants were calculated. Because the isotherm curves obtained for the adsorption of O-II and CV by chitosan and of CV by MCM-chitosan fit the Langmuir adsorption isotherm, b constants were applied to thermodynamic equations, and thermodynamic parameters (delta G, delta H, and delta S) were calculated. Lastly, chitosan and MCM-chitosan were compared with respect to the ability to take up the dyestuffs and PNP.     

茶多酚锰-壳聚糖微球的制备、控释和诱导肝癌细胞凋亡的研究

选用壳聚糖为微米粒包被材料, 制备茶多酚锰(Tea Polyphenol Manganese, TPMn)-壳聚糖微球. 用荧光显微技术研究了TPMn-壳聚糖微球的荧光特性, 用扫描和透射电子显微镜证实TPMn-壳聚糖微球尺寸和分布规律. RP-HPLC定量分析TPMn-壳聚糖微球包封率为68%, 符合微米级微粒控释药物包封率的要求. 动力学研究结果表明, 茶多酚(TP)-壳聚糖和TPMn-壳聚糖的微球均有控释TP的能力, 控释时间高达40 h以上, 但前者释放速率稍快于后者. TPMn和TPMn-壳聚糖微球均能诱导肝癌细胞凋亡, 但TPMn-壳聚糖微球诱导肿瘤细胞的凋亡速率稍高于TPM. 实验结果证实, 以TPMn-壳聚糖微球方式控释TPMn有利于提高诱导肿瘤细胞凋亡速率. TPMn-壳聚糖微球具有研发成注射型抗肿瘤新药的可能性.