共查询到20条相似文献,搜索用时 109 毫秒
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首次利用漆酶-乙酰丙酮(AA)-过硫酸钾组成的复合引发体系在室温水相中引发丙烯酰胺的聚合, 采用滴球法将上述反应液滴入硫酸铜溶液, 利用铜离子与壳聚糖的络合交联反应制备得到尺寸可控的核壳结构小球. 小球内部发生自由基聚合反应, 将漆酶-AA同步固定于新生成的具有三维网络结构的水凝胶中. 微反应器小球的形貌和内部孔道结构通过扫描电镜和氮气吸附实验进行了表征. 该固定化漆酶-介体小球微反应器相比于游离漆酶表现出更高的pH稳定性和耐热性. 得益于固定化AA的介导作用, 漆酶的可重复使用性能得到显著提升, 降解孔雀石绿的有效循环批次较游离漆酶-介体体系延长了3倍, 为降低漆酶在实际废水处理中的成本、削减游离介体带来的二次污染提供了一条有效途径. 相似文献
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利用离子液体溶解纤维素混合体系固定化介体2,2'-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS),介体分子被封装于具有三维网络通道结构的纤维素微球中.利用扫描电子显微镜(SEM)、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)等表征了微球的形貌、结构和组成.利用多巴胺自聚合作用将漆酶固定在封装介体的纤维素微球上,实现了漆酶与介体的共固定.由于固定化ABTS的介导作用,制备的球状共固定漆酶-ABTS催化剂,对吲哚的降解率达到99%以上,并呈现出相比于游离漆酶更高的耐热性和耐酸碱性.球状共固定漆酶-ABTS催化剂可重复用于吲哚的生物降解,连续循环使用9次,吲哚的降解率保持在89.6%. 相似文献
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胶束介质在动力学分析中的应用 总被引:8,自引:1,他引:8
评述了胶束介质在动力学分析测定中的作用和应用状况,讨论了胶束催化的动力学特征,胶束对反应物理化学性质的修饰及其在动力学多组份测定和酶催化动力学分析中的应用。 相似文献
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《分析试验室》2017,(6)
利用电化学还原氧化石墨烯(GO)的方法将还原石墨烯(erGO)沉积在电极表面上,然后电沉积氧化锌纳米棒(ZnO)构成erGO/ZnO复合纳米材料修饰玻碳电极(GCE),最后通过电聚合中性红(NR)电子介体包埋法将辣根过氧化物酶(HRP)固定在GCE/erGO/ZnO表面制得GCE/erGO/ZnO/HRP-PNR(聚中性红)。用SEM和能谱对复合纳米材料进行了表征,通过电化学阻抗法和循环伏安法对修饰电极进行了探究,通过循环伏安法和计时电流法研究了GCE/erGO/ZnO/HRP-PNR对有机过氧化物的催化性能,结果该修饰电极对过氧化氢叔丁基(BHP)和过氧化氢异丙苯(CHP)都具有良好的检测性能。 相似文献
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酶催化包括底物到活性区的输运、选择催化化学反应及产物释放等复杂过程,由于复杂的蛋白质环境效应,任一化学和非化学过程都有可能是决定酶活性的关键步骤。为了全面认识酶催化活性,我们对几类酶催化过程进行了广泛的组合量子/分子力学(QM/MM)和经典分子力学(MM)动力学模拟(MD)研究,详细地讨论了整个酶催化过程的分子机制、关键残基的作用和蛋白质环境效应,丰富了对酶催化活性的认识。随着多尺度模型和计算模拟方法的进一步完善与发展,有望实现超大复杂生物酶催化过程的全程模拟研究,为酶工程领域的相关研究提供支持。 相似文献
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研究了PtCl^2^-6接着PVP修饰电极及其催化动力学,用循环伏安法考察了电极的电化学性质,结果表明在酸性溶液中对Fe^2^+的氧化有显著的催化作用。采用旋转圆盘电极法,极化曲线法等就电极对Fe^2^+氧化催化过程的控制步骤及有关动力学参数进行了测定。 相似文献
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《Analytical letters》2012,45(8):1339-1357
Abstract An amperometric enzyme electrode for the determination of glucose (<16mmol?1) is constructed by incorporating electron mediators tetrathiafulvalene in Nafion and subsequently coating with immobilized glucose oxidase. The results obtained from measurements of glucose in fermentation samples containing biomass or molasses indicate the utility of the electrode for glucose assay in such media over at least 12 weeks. Digital simulation is employed to study the cyclic voltammetry (CV) of Tetrathiafulvalene-Glucose Oxidase-Modified Glassy Carbon Electrode (TTF-GOD-GCE); the digital model is built and the effects of kinetic parameters on CV-curves are discussed. The response process of the Tetrathiafulvalene-Glucose Oxidase-Modified Electrode is partly explained by the simulation results and further research is expected to guide the design of biosensors and improve the properties of the enzyme-mediator modified electrode. 相似文献
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金属纳米粒子由于其小的体积和大的比表面积而具有独特的电子、光学和异相催化特性,是目前表面纳米工程及功能化纳米结构制备的一种理想研究对象[1]。银纳米粒子可广泛应用于催化剂材料、电池的电极材料、低温导热材料和导电材料等,成为近年来人们研究的热点[2,3]。在电化学方面,银纳米粒子具有比其他纳米粒子更为优异的导电性能和电催化性能。因此,研究银纳米粒子修饰电极有重要的应用价值和前景[4]。1实验部分1.1仪器CHI660电化学工作站(USA);TU-1901型双光束紫外可见分光光度计(北京普析通用仪器公司);KQ-100型超声清洗器(昆山市超声… 相似文献
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Simson Prasanna Kumar Revanasiddappa Manjunatha Thimmappa Venkatarangaiah Venkatesha Gurukar Shivappa Suresh 《Russian Journal of Electrochemistry》2013,49(4):299-306
Graphite electrode is modified by casting multi-walled carbon nanotubes (MWCNTs) wrapped with polystyrene sulphonate (PSS) onto the surface of the bare graphite electrode. The modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The behavior of the modified electrode towards the oxidation of ascorbic acid (AA), dopamine (DA) and uric acid (UA) has been determined by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry (CA). The modified electrode showed better electrocatalytic activity towards AA, DA and UA compared to bare graphite electrode. The electrochemical oxidation signals of AA, DA and UA are well separated into three distinct peaks with peak potential difference of 222, 128 and 350 mV between AA-DA, DA-UA and AA-UA respectively in CV studies and corresponding peak potential separation in DPV are 228, 120 and 348 mV. This modified electrode was successfully used for simultaneous determination of AA, DA and UA in ternary mixture. 相似文献
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Cecchet F Marcaccio M Margotti M Paolucci F Rapino S Rudolf P 《The journal of physical chemistry. B》2006,110(5):2241-2248
Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and digital simulation techniques were used to investigate quantitatively the mechanism of electron transfer (ET) through densely packed and well-ordered self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid on gold, either pristine or modified by physically adsorbed glucose oxidase (GOx). In the presence of ferrocenylmethanol (FcMeOH) as a redox mediator, ET kinetics involving either solution-phase hydrophilic redox probes such as [Fe(CN)6]3-/4- or surface-immobilized GOx is greatly accelerated: [Fe(CN)6]3-/4- undergoes diffusion-controlled ET, while the enzymatic electrochemical conversion of glucose to gluconolactone is efficiently sustained by FcMeOH. Analysis of the results, also including the digital simulation of CV and EIS data, showed the prevalence of an ET mechanism according to the so-called membrane model that comprises the permeation of the redox mediator within the SAM and the intermolecular ET to the redox probe located outside the monolayer. The analysis of the catalytic current generated at the GOx/SAM electrode in the presence of glucose and FcMeOH allowed the high surface protein coverage suggested by X-ray photoelectron spectroscopy (XPS) measurements to be confirmed. 相似文献
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Hanane Zejli Jose Luis Hidalgo-Hidalgo de Cisneros Khalid Riffi Temsamani 《Talanta》2010,80(5):1805-1808
The Sonogel electrode was immersed in hemin solution to obtain the hemin-Sonogel-modified electrode by cyclic voltammetry (CV). It was applied to detect superoxide radicals produced by xanthine oxidase catalyzed hypoxanthine oxidation. The characterization of hemin-Sonogel was modified by impedance and CV. 相似文献
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通过电沉积的方式在多壁碳纳米管(MWCNTs)修饰玻碳电极表面上沉积铂(pt)纳米粒子,并运用循环伏安法(CV)、示差脉冲伏安法(DPV)探讨了芦丁在铂纳米/碳纳米管/玻碳电极上的电化学行为.实验结果表明,芦丁在该修饰电极上呈现一对良好氧化还原峰,其氧化峰电流与浓度在3.2×10(-8)~1.2×10(-5)mol/L... 相似文献
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The electrocatalytic oxidation of nitric oxide(NO) at a glass carbon electrode(GC) modified with functionalized single-walled carbon nanotubes(SWCNTs) was investigated by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS).It was found that the SWCNT modified electrode could speed greatly up the electron transfer rate compared with the bare GC electrode.After the SWCNT was treated with alkali or mixed acids,the reaction rate and activation energy of NO electrooxidation were changed to different extent.Chemical modification of the SWCNT surface is one of the most powerful methods to change the sensitivity of NO electrooxidation reaction.The modified electrode with SWCNT obtained by the firstly alkali treatment and then the mixed acids treatment was the best one for NO electrooxidation,the result of CV was also confirmed by that of EIS.The anodic processes of NO were recognized more clearly by exploring the reaction mechanism of NO electrooxidation at the SWCNT modified electrode. 相似文献
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<正>Poly-L-lysine(PLL) was first electrodeposited onto the surface of a glassy carbon(GC) electrode.The PLL modified electrode was used to immobilize chloroperoxidase(CPO) via 1-[(3-dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride(EDC).The electrochemical behaviors of immobilized CPO on PLL/GC electrode were investigated by cyclic voltammetry(CV).The CV results obtained showed that CPO was successfully immobilized on the PLL/GC electrode and a fast direct electron transfer between CPO and PLL-GC electrode was achieved with a formal redox potential of -0.23 V vs.SCE.The CPO-PLL/GC modified electrode showed a good catalytic activity for electrocatalytical reduction of O_2,promising for a broad range of CPO-catalyzed transformations. 相似文献