浙江省建立新生儿TSH血样采集邮寄检验服务网络的策略与评价

为研究新生儿促甲状腺激素（ＴＳＨ）血样采集方法,建立了采集邮寄服务网络,提高ＴＳＨ检验工作水平和覆盖率。结果表明,发挥网络功能对开展新生儿ＴＳＨ检验工作是非常重要的。     

碘缺乏病病区胎儿及孕妇硒含量和硒—谷胱甘肽过氧化物酶活性研究

测定了麻江县和贵出市郊两地胎儿组织，胎儿和孕妇血硒含量和谷胱甘肽过氧化物酶活性，同时测定了生态环境中硒含量，结果发现，麻江地区环境硒含量低，孕妇血硒水平和红细胞ＧＰｘ活性明显低于贵阳地区，胎儿组织及血Ｓｅ含量和ＧＰｘ活性也有一致性低于贵阳地区的趋势显示麻江人群因摄硒不足而存在的硒代谢紊乱。调查的两地区均连续碘盐防治十年以上，但麻江地区人群仍处在高促甲状腺激素水平，学龄儿童甲肿率还显著高于贵阳，这些     

碘的生物无机化学研究进展—碘对甲状腺激素生物活性的结构作用探讨

本文综述甲状腺激素相关研究的进展 ,探讨碘在甲状腺激素与细胞内激素受体蛋白结合中的结构作用。文中评述了关于甲状腺激素与血浆运载蛋白和细胞内受体蛋白结合的溶液亲合性、结构研究和甲状腺激素碘代酚环的分子识别特性与碘的结构效应模拟研究的数据结果 ,并就碘对激素 受体蛋白结合的结构作用提出了作者的观点     

碘的生物无机化学研究进展—碘对甲状腺激素生物活性的结构作用探讨

本文综述甲状腺激素相关研究的进展,探讨碘在甲状腺激素与细胞内激素受体蛋白结合中的结构作用。文中评述了关于甲状腺激素与血浆运载蛋白和细胞内受体蛋白结合的溶液亲合性、结构研究和甲状腺激素碘代酚环的分子识别特性与碘的结构效应模拟研究的数据结果,并就碘对激素受体蛋白结合的结构作用提出了作者的观点。     

游离和溶胶-凝胶固定化甲醛脱氢酶酶促反应动力学的研究

采用溶胶-凝胶法对甲醛脱氢酶进行固定化，酶的包埋率超过了98％．在pH7附近、37℃下，以游离酶和固定化酶作催化剂，NADH为电子供体，进行了甲酸转化为甲醛的酶促反应．游离和固定化甲醛脱氢酶酶促反应都遵循Michaelis-Menten反应机理，用Dalziel提出的双底物酶促反应动力学方程进行拟合．固定化酶酶促反应速率为游离酶酶促反应速率的50％左右．固定化酶的动力学常数φ和米氏常数K高于游离酶，估计是凝胶基质孔中存在扩散效应所致．     

流动注射化学发光测定甲状腺素的含量

甲状腺素(T4)是甲状腺滤泡细胞合成及分泌的激素,以游离形式释放入血循环中,并迅速与血浆蛋白相结合,并对于人体中枢神经系统,婴幼儿骨骼生长和器官正常发育起着重要的作用.临床上,T4可以作为克汀病、垂体性甲状腺功能减退症和体促甲状腺激素肿瘤等病症的病情监测指标.因此,T4的灵敏检测具有重要意义.测定T4的方法有高效液相色谱~([1])、化学发光~([2])、免疫~([3])和荧光方法~([4])等.本文报道了流动注射化学发光检测T4含量的新方法.     

3,5-二甲基-D,L-酪氨酸与铜(Ⅱ)和芳香氮碱配体形成混配配合物的稳定性研究

碘是脊椎动物生命过程中重要的必需元素之一,被动物体吸收后主要用于甲状腺激素的生物合成.甲状腺激素与血浆中运载蛋白和细胞内受体蛋白结合的溶液亲和性及结构研究表明,碘具有结构作用[1].     

2型糖尿病患者血清钙镁与甲状腺激素含量变化及临床意义

为了监测 2型糖尿病人血清钙、镁和甲状腺激素含量 ,分析其临床意义及相关性 ,应用放射免疫分析法测定了 1 1 5例 2型糖尿病及 1 5 0例健康人血清甲状腺激素含量。同时用美国杜邦RXL自动生化仪测定了其血清钙、镁含量。结果表明 ,在 2型糖尿病伴有明显并发症者血镁、FT3水平明显降低 (P <0 0 5 ) ,钙镁两种元素与甲状腺激素水平无明显相关性。 2型糖尿病患者适当补镁对预防其并发症是有益的 ,测定FT3 等可作为判断 2型糖尿病严重程度和估计预后的参考指标。     

离子液体的制备及其在酶催化反应中的应用

离子液体，尤其是非水溶性离子液体可以作为一种溶剂或酶的载体用于非水相酶促反应中，也可以用于双相体系中的酶促反应。本文概括性介绍了常见离子液体的制备，总结和讨论了离子液体中酶的活性、稳定性、反应选择性以及各类酶在离子液体中的催化反应行为。离子液体的物性及其与酶的相容性对酶本身及酶促反应都有很大的影响。在非水相酶促反应中，离子液体的极性作用不遵从通常用来判别大多数有机物溶剂行为的规则，比如lgP规则。     

超促排卵治疗后卵泡破裂合并官外孕一例

一名继发不孕，多囊卵巢病史的妇女接受ＨＭＧ和ＨＣＧ超促排卵治疗后，发生了并发症，即卵泡破裂出血，宫外孕，卵巢过度刺激综合征等。提示进行超促卵治疗时，必须合理使用排卵药及加强监测手段，以促证治疗的安全性。     

Factors affecting the serum thyroid hormone concentration during fasting in rats--with special reference to the relation with temperature

The present studies examine the effect of starvation together with cold or hot exposure on thyroid hormone levels in rats. At 23 degrees C starved for 5 days, serum thyroid hormone levels decreased significantly compared with fed rats, averaging 3.6 +/- 0.5 micrograms/dl of thyroxine (T4), 47 +/- 11 ng/dl of triiodothyronine (T3), 1.4 +/- 0.3 ng/dl of free T4 and 39.6 +/- 5.1 pg/ml of reverse T3, respectively. At 15 degrees C rats starved for 5 days, serum free T4 level significantly more increased than that of 23 degrees C starved rats, while serum T4 level and T3 did not increase significantly. At 30 degrees C rats whether concomitant starvation or not, serum thyroid hormone levels of both group markedly more decreased than control rats. These experiment provide additional evidence that thyroid gland and the peripheral metabolism of thyroid hormone respond to variety situations such as cold or hot exposure together with starvation or not.     

侧脑室注射氯化镧对大鼠血清生长素和甲状腺素的影响

西方研究了侧脑室注入ＬａＣｌ３对大鼠血清中生长系,甲状腺素,促甲状腺素和下丘脑中生长抑素的影响。侧脑室注射０．００１和０．０１ｍｏｌ．ｌ＾－１ＬａＣｌ３,血清中Ｔ４和ＧＨ含量明显高于对照组,０．１和０．５ｍｏｌ．ｌ＾－１ＬａＣｌ３组血清Ｔ４和ＧＨ未见明显变化。     

Development of a functional impedimetric immunosensor for accurate detection of thyroid-stimulating hormone

Thyroid-stimulating hormone (TSH), which regulates the synthesis of thyroid gland hormones affecting the whole metabolism, is a pituitary hormone. Determination of TSH is crucial for monitoring thyroid gland-related disorders and some metabolic diseases.In this study, a nonlabeled immunosensor based on covalent immobilization of anti-TSH antibody by using the formation of self-assembled monolayers (SAM) of 4-mercaptophenylacetic acid (4-MPA) and functionalization of carboxyl ends with 1-ethyl-3-(3-dimetilaminopropil) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) was fabricated for detection of TSH. Immobilization steps including the concentration of 4-MPA, the concentration of anti-TSH antibody, and duration of anti-TSH antibody incubation were optimized by utilizing electrochemical impedance spectroscopy. Under optimal conditions, a sensitive, rapid, and accurate determination of TSH at a concentration range between 0.7 and 3.5 mIU/L was accomplished with a notable linearity and LOD value of 0.034 mIU/L, as well as reproducibility and repeatability. Moreover, for comparison, linear range experiments were also carried out by using other electrochemical methods, including linear sweep voltammetry, cyclic voltammetry, and capacitance spectroscopy. Finally, the constructed immunosensor was used for analyzing TSH levels spiked in the artificial serum samples.     

Basic evaluation of highly sensitive thyroid stimulating hormone immunoradiometric assay kits

Usefulness of three kinds of TSH kits by immunoradiometric assay (IRMA) was evaluated. They were able to measure low levels (less than 0.1 microIU/ml) in serum thyroid stimulating hormone (TSH) with incubation of short time (4 hours). In particular, RIABEAD II kit had a highly specific affinity for TSH and the normal range (+/- 2 S.D.) using it showed from 0.20 to 3.50 microIU/ml in 150 normal subjects. In patients with hyperthyroidism and in patients with hypothyroidism, the values of TSH were lower and higher than those of normal subjects, respectively. Another kits showed similar results. These results indicate that these TSH-IRMA kits are useful to evaluate serum TSH levels exactly.     

The investigation of thyroid function.

The measurement of thyrotropin (TSH) by a third-generation assay (functional sensitivity 0.01-0.02 mU/L) offers the single best test of assessing thyroid status and is more cost effective than measurement of thyroxine (T4) as a first-line test. However, in certain situations, e.g., nonthyroidal illness (NTI), secondary thyroid disease, early treatment of hyperthyroidism, early pregnancy, and heterophilic antibody interference, TSH alone may be misleading, and thyroid hormone measurements are also required. The author's laboratory has found that the measurement of TSH together with free thyroxine (by a nonanalogue method) offers the most speedy and effective first-line strategy for thyroid function testing. Abnormalities in thyroid function tests in euthyroid patients are most likely found in NTI and the elderly. For this reason, thyroid function tests (TFTs) should not be requested in elderly or hospitalized patients unless the presenting complaint is considered to be because of a thyroid problem.     

甲状腺功能亢进症患者血清铜、锌、铁、钙、镁的研究

为研究甲状腺功能亢进症患者血清中 5种微量元素的变化及其与甲状腺功能激素间的关系 ,用分光光度分析和放射免疫分析分别检测了 1 2 2例甲状腺疾病患者血清中微量元素Cu、Zn、Fe、Ca、Mg含量和血清中甲状腺激素 (T3、T4 )水平。结果表明 ,甲状腺功能亢进症患者血清中Cu、Ca元素水平明显高于对照组 (P <0 0 1 ) ,Zn元素水平明显低于对照组 (P <0 0 0 1 ) ,Fe、Mg元素两组比较无统计学意义 ;血清Cu与T3、T4 浓度呈现一定程度的正相关 ,提示微量元素在甲状腺激素的合成、代谢和生物作用中可能有重要意义 ,甲状腺功能亢进症患者过多释放甲状腺素导致血清中元素的异常变化 ,此变化既能反映病人机体代谢紊乱程度 ,同时也可作为病情控制的参考指标。     

Pharmacological properties of galenical preparation. XV. Pharmacokinetics study of evocarpine and its metabolite in rats.

It is known that when methanol extract of Evodia fruit is orally administered, 5-(1,4-dihydro-1-methyl-4-oxo-2-quinolin-2-yl) pentanoic acid (EVCA) is excreated as a matabolite in rat urine. In this study, we separated Evodia fruit extract into major alkaloids administered each alkaloid individually to male Wistar rats. Consequently, it was demonstrated that the original substance of the metabolite are evocarpine and its analogues, dihydroevocarpine and 1-methyl-2-undecenyl-4(1H)-quinolone. Investigation of a blood sample after oral administration of evocarpine by high performance liquid chromatography confirmed that the substance was absorbed without alteration. Pharmacokinetics of evocarpine after intravenous injection was expressed in a one-compartment model, showing a linear elimination of plasma evocarpine up to a dosage of 75 mg/kg. Total plasma clearance (CL), volume of distribution (Vd), and half-life (T1/2) of evocarpine were 60 ml/min.kg, 3.21/kg and 0.6 h-1, respectively. Metabolic ratio of evocarpine into EVCA after intravenous injection was 15.4%, and absorption ratio of the unaltered compound calculated from the levels of AUC after oral administration and intravenous injection was 4.7%. In this paper, it is shown that evocarpine is absorbed amount 100% when it is administered orally.     

Fundamental studies on thyroid stimulating hormone immunoradiometric assay kit (TSH RIABEAD II)

We have reported fundamental studies on the TSH immunoradiometric assay, using TSH RIABEAD II kit (Dainabot). The sensitivity of the assay was 0.03 mu IU/ml and its C.V. was 27.2%. Intra- and inter-assay C.V. were less than 5%. Dilution test and recovery test were good. Serum TSH level was 0.3-4.0 mu IU/ml in normal subjects, less than 0.03 mu IU/ml in untreated Graves' disease and subacute thyroiditis. Therefore, it was found that the clear difference exist in serum TSH levels between normal subjects and patients with untreated Graves' disease. There was a well correlation on the serum TSH levels between this method and TSH radioimmunoassay kit (Amerlex TSH, r = 0.983). Especially, the measurement of serum TSH levels, using immunoradiometric assay kit, was useful for the diagnosis of patients with Graves' disease.     

Application of adaptive pressure-driven microfluidic chip in thyroid function measurement

The improvement in accuracy of in vitro diagnosis has always been the focus of early screening of thyroid dysfunction. We constructed a microfluidic chip based on a polystyrene polymer substrate. Total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3), free thyroxine (FT4), and thyrotropin (TSH) in human whole blood samples were analysed by fluorescence immunoassay to evaluate thyroid function. The results indicate that the microfluidic chip shows a good linear relationship in the detection of TT3, TT4, FT3, FT4, and TSH standards, and the correlation coefficient (r) is not less than 0.9900. In addition, the chip also has strong anti-interference (RSD% ≤ 5%) and good repeatability (CV ≤ 8%), and its inter-batch differences are small (CV ≤ 15%). The results of practical application in clinical thyroid function measurement indicated its high accuracy (r ≥ 0.9900). It provides a new method for the determination of thyroid function and lays a foundation for subsequent clinical application.