共查询到19条相似文献,搜索用时 366 毫秒
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光度法测定甲基橙和二甲基黄在甲醇—水混合溶剂中的离解常数 总被引:1,自引:0,他引:1
利用光度法测定了酸碱指示剂甲基橙(MO)和二甲基黄(DY)在0%~90%(V/V)和35%~84%(V/V)甲醇-水混合溶剂中的离散常数pKa(25℃,离子强度I=0.1mol/LKCl)实验结果表明,随着混合溶剂中甲醇含量的增加,两种指示剂的pKa均不断减小,当混合溶液中甲醇含量小于75%时,pKa同甲醇的摩尔分数X2之间存在着良好的线性关系。甲醇的加入,指示剂在可见光区的吸收光谱有较明显的改变 相似文献
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本文利用电位滴定法测定了25℃,KCl离子强度I=0.1mol.L^-1时,甘氨酸在纯水及8个乙醇-水混合溶剂中的二级离解常数pKa,对应的碱离解常数pKb和混合溶剂的质子自递常数pK。实验结果表明:在所研究的浓度范围内,随着混合溶剂中乙醇含量的增加,甘氨酸的酸离解常数及溶剂的质子自递常数逐渐减小,碱离解常数则逐渐增大。 相似文献
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在25±0.1℃,I=0.1mol·dm-3KNO3条件下,80%(v/v)DMSO—H2O混合溶剂中,应用pH法测定了甘氨酸,L一缬氨酸,L-异亮氨酸,L-脯氨酸,L-丝氨酸,DL-苯丙氨酸等六种α-氨基酸(缩写为α-AA,记为B配体)的酸离解常数,铜(Ⅱ)-α-氨基酸.锌(Ⅱ)-α-氨基酸二元配合物的稳定常数及铜(Ⅱ)-芬布芬-α-氨基酸(芬布芬缩写为Fen,记为A配体)和锌(Ⅱ)-芬布芬-α-氨基酸三元配合物的稳定常数.实验发现,在下面三对参数之间,即logβ102与pK,lOgβ111;与pK及10gβ111与logβ102,均存在良好的直线自由能关系.用△logKM和△logβ111两个参量描述了三元配合物相对于母体二元配合物的稳定性.讨论了溶剂的性质,配合物分子内配体之间的疏水作用,芳环之间的堆积作用对配合物稳定性的影响. 相似文献
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在298.在5K,对四苯硼钾在不同比例的水-乙醇混合溶剂顺的活度积及从水到混合溶剂中的标准迁移自由 能进行了研究,并对四苯硼钾在水-乙醇混合溶液中的介质效应进行了分析讨论 。 相似文献
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铜(Ⅱ)—α—氨基酸(5—取代邻菲咯啉)—L—苏糖酸三元配合物的稳定性研究 总被引:1,自引:0,他引:1
孙祥德 《高等学校化学学报》1998,19(6):849-853
在298K,I=0.1MOL/lKNO3条件下测定了L-苏糖酸-α-氨基酸-铜(Ⅱ)三元配合物的生成常数。实验表明,生物配体α-氨基酸及L_苏糖酸具有良好的相容性,其三元配合物的稳定性大于相应二元配合物的稳定性。 相似文献
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柱前衍生反相高效液相色谱分离和测定氨基酸:用N—羟基琥珀酰?… 总被引:5,自引:0,他引:5
以N-羟基琥珀酰亚胺-α-萘乙酸酯(SINA)为氨基酸的柱前衍生试剂,反相高效液相色谱分离测定了15种氨基酸。采用含10mmol/L pH5.0的乙酸-乙酸钠缓冲溶液的甲醇-乙酸乙酯-水(10/2/88,V/V/V)溶液为流动相体系。分离测定了7种氨基酸;用甲醇-乙酸乙酯-水(26/2/77,V/V/V)分离测定了5种氨基酸;用甲醇-乙酸乙酯-水(45/2/53,V/V/V)分离测定了3种氨基酸。 相似文献
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以N—保护消旋氨基酸为羧基组分的酶促合成肽的研究 总被引:3,自引:0,他引:3
用不同的氨基保护的消旋氨基酸或酯作为羧基组分,在α-胰凝乳蛋白酶或木瓜蛋白酶的催化下与另一氨基酸的酰胺或酯综合形成的预期的光活性肽产物,以L-构型底物计算肽的产率为55%-83%。化合物的物理学常数与用相应的N-保护-L-氨基酸或酯为羧基组合组分,分别用化学法或酶促法测定所得结果一致,研究结果表明,利用酶的立体专一性,在形成肽键的过程中进行立体选择可得到光学活性的产物而不需预先拆分;只要溶剂体系中 相似文献
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盐溶液对蛋白质的结构和性质(例如蛋白质的溶解度、变性、解离为次级结构以及酶的活性’‘’等)有很大的影响.由于蛋白质是一个结构复杂的大分子,直接研究它与电解质的相互作用比较困难.因而,对蛋白质的模型化合物氨基酸和肽的研究具有重要的意义.关于氨基酸在氯化钙、碱金属卤化物(LICI,NaCI,KCI,CsCI,KBr,KI)、硫氨酸钾和氯化控水溶液中的体积性质已有文献报导’‘-”.丁酸钠是一种有机盐,丁酸根离子具有疏水的丙基和亲水的核基,因而它对氨基酸的作用与简单阴离子的作用可能会有所不同.另外,丁酸钠在水溶液中… 相似文献
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A new method for separation of several amino acids using mobile phases (MPs) containing mixed micelles as well as 1-butanol and aprotic organic solvents as modifying additives has been developed. The effect of experimental factors (the ratio of MP components, nature of stationary phase) on separation of amino acids is investigated and all conditions for separation are optimized. It was found that separation of three amino acids l-lysine, l-histidine and l-tryptophan on silica gel stationary phase in the interval from micrograms to milligrams is available. The proposed method is simple and allows determining the amino acids in the linear interval of 0.1-1.5mg. 相似文献
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应用精密数字密度计测定了298.15 K时甘氨酸、L-丙氨酸或L-缬氨酸与不同组成的木糖醇-水混合溶剂构成的三元系溶液的密度, 计算了氨基酸的表观摩尔体积、极限偏摩尔体积、迁移偏摩尔体积和水化数. 根据结构水合作用模型讨论了迁移偏摩尔体积和水化数的变化规律. 结果表明, 氨基酸两性离子部分与木糖醇羟基间的相互作用占主导地位, 且随木糖醇浓度的增大而增大. 氨基酸在木糖醇水溶液中的迁移偏摩尔体积为正值, 甘氨酸的迁移偏摩尔体积大于L-丙氨酸和L-缬氨酸的. 氨基酸在木糖醇水溶液中的水化数随溶液中木糖醇浓度的增加而减小. 相似文献
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《Physics and Chemistry of Liquids》2012,50(4):369-382
Apparent molar volumes, viscosity B-coefficients, and apparent molar isentropic compressibilities of glycine, L-alanine, L-valine and L-leucine in 0.062, 0.125 and 0.256 mol kg?1 aqueous tetra-butyl ammonium bromide (TBAB) solution have been determined at 298.15 K from their experimental density, flow time and sound speed measurements, respectively. The standard partial molar volumes and compressibilities are used to calculate the corresponding volume of transfer at infinite dilution, from water to aqueous TBAB solutions. The linear correlation of partial molar volumes for a homologous series of amino acids has been utilised to calculate the contribution of charged end groups and other alkyl chains of the amino acids to partial molar volumes. The hydration numbers of amino acids have also been determined. Viscosity B-coefficients have been calculated using the Jones–Dole equation. The values of the charged end groups contribution to the viscosity B-coefficients of the amino acids are calculated. 相似文献
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Elena V. Parfenyuk Olga I. Davydova Nataliya Sh. Lebedeva 《Journal of solution chemistry》2004,33(1):1-10
Calorimetric titrations have been performed at 298.15 K in aqueous solutions to derive the stability constants and thermodynamic parameters of the interactions of D-maltose and sucrose with some amino acids (glycine, DL-alanine, DL-leucine, and L-serine). The apparent molal volumes of the disaccharides in dilute aqueous solutions of the amino acids have been determined from density measurements at 298.15 K. In contrast to D-maltose, sucrose was found to associate with the amino acids and these associated species are preferentially entropy stabilized. These results are interpreted in terms of the influence of the nature of the solutes, their specific conformations, and hydration, on the ability of the disaccharides to form associated complexes with the amino acids. 相似文献
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建立了水溶液中脂肪族α- 氨基酸疏水自缔合相互作用的化学模型, 根据模型方程对由精密流动微量热法测得的α- 氨基酸水溶液的稀释焓数据进行回归分析, 得到等步自缔合作用的平衡常数(K)、焓变(ΔHm)和熵变(ΔSm)等热力学参数, 发现焓、熵之间存在很好的补偿关系. 同时计算了溶液中水的偏摩尔过量熵(SE1), 并根据脂肪族α- 氨基酸的水化模型对结果进行了讨论. 建立的化学模型参数能在一定程度上解释McMillan- Mayer模型中的同系焓作用系数的物理意义. 相似文献
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《Physics and Chemistry of Liquids》2012,50(1):77-84
Abstract The solubilities of amino acids at 288.15, 298.15 and 308.15K in water and aqueous sodium sulfate have been determined as a function of electrolyte concentration (0.5, 1.0, 1.5 mol/kg of water). It has been observed that sodium sulfate renders amino acids less scluble. The free energies of transfer of amino acids from water to aqueous sodium sulfate have been calculated from the solubility data. The enthalpies and entropies of transfer have also been estimated. The solubility and the thermodynamics of transfer data manifest that sodium sulfate generates favourable interaction between nonpolar groups of amino acids. If applied to proteins, it seems to stabilize the protein native globular structure by enhancing the intramolecular nonpolar-nonpolar group interactions. 相似文献