显微荧光—阿达玛变换三维图象分析

把阿达玛变换多通道成象技术与荧光显微镜结合起来，首次成功地获得了细胞的阿达玛变换三维荧光图象。该图象能同时提供微小物体中某一物质的含量及分布等信息。     

双编码阿达玛变换光谱法：原理,仪器及光谱分析研究

本文根据阿达玛变换的原理研制成一种双编码阿达玛变换光谱仪，与单编码及常规光谱仪相比，该仪器具有高信噪比、高输入和单检测器多通道同时检测的优点。试样分析表明：该仪器能用于荧光谱分析和原子线光谱分析。     

阿达玛变换显微图象分析（Ⅱ）—荧光衰减对图象和定量分…

采用计算机模拟的方法，系统地考察了试样的荧光衰减对阿达玛变换图象和定量分析数据的影响，并把阿达玛变换显微图象分析仪用于细胞内ＤＮＡ的定量分析，得到好满意的结果。     

阿达玛变换光谱和成像技术的应用和研究进展

阿达玛变换(HT)作为一种多通道光谱调制技术，具有多通道同时检测能力、多通道成像能力以及适用于数据处理等优点。综述了近十年来HT光谱和成像技术在分析科学中的应用和研究进展。主要从HT模板编码技术和HT激发序列应用技术等方面讨论了其最新发展和存在的问题，并展望了其发展前景。     

显微荧光－阿达玛变换三维图象分析

把阿达玛变换多通道成象技术与荧光显微镜结合起来,首次成功地获得了细胞的阿达玛变换三维荧光图象,该图象能同时提供微小物体中某一物质的含量及分布等信息。     

使用一维机械模板的高分辨阿达玛变换显微图像探测技术研究

阿达玛变换（HT）是一种类似于傅里叶变换（FT）的光谱调制技术,具有多通道同时检测和多通道成像能力等优点,但两者的数学模型、对光信号的调制方法和调制手段都不一样。由于HT仅涉及四则运算,而FT涉及较为复杂的三角函数和复数运算,所以HT的解码速度快于FT。在成像技术方面,HT具有直接成像的能力,而FT只能对通过其它方式获取的图像进行加工处理。     

阿达玛变换显微图象分析(Ⅱ)──荧光衰减对图象和定量分析数据的影响

采用计算机模拟的方法,系统地考察了试样的荧光衰减对阿达玛变换图象和定量分析数据的影响,并把阿达玛变换显微图象分析仪用于细胞内DNA的定量分析,得到了满意的结果。     

阿达玛变换显微图像分析系统在乳腺肿瘤细胞DNA倍性分析…

以正常人外周静脉血淋巴细胞为标准二倍体细胞，以吖橙为细胞ＤＮＡ荧光探针，用阿达玛变换显微图象分析仪测定了冰例乳腺肿瘤的细胞ＤＮＡ倍性与面积呈显著正相关。     

高分辨阿达玛变换显微荧光图像分析(Ⅱ)单细胞DNA荧光图像生成、处理及自动定量分析研究

本研究把荧光显微镜，阿达玛变换多通道成像技术和计算机图象处理技术结合起来，建立了一种单细胞DNA荧光图像自动定量分析系统，对系统的成像原理、信号编码和解码方法及单细胞定量分析方法进行了讨论，分析结果表明，本系统提供的单细胞定量分析数据稳定可靠。     

高分辨阿达玛变换显微光谱成像系统研究

阿达玛变换(HT)是一种多通道的光谱调制技术,基于这一原理,研制了一种显微光谱成像系统.通过光学设计,除可以获得微小试样(如单个细胞)的光谱以外,还可以对试样进行分光谱成像,得到分辨率为256×256像素的256级灰度图像.通过对两种不同荧光发射波长的CdSe/ZnS量子点进行的分光谱成像测试表明,本系统具有良好的分光谱成像能力,可以对具有复杂光谱的物质针对其某一特定成分进行成像.     

Studies on Hadamard Transform Fluorimetry

Hadamard transform spectroscopy has been investigated since the later 1960's and early 1970's. It was demonstrated that the application of Hadamard transform technique to spectroscopy can facilitate multichannel detection of weak signal. It can be appplied not only to component analysis, but to image analysis. Now, it has been widely used in infrared spectrometry, Raman spectrometry and photothermal deflection analysis. But its application to molecular fluorimetry has not been reported up to now.     

Instrument for Hadamard transform three-dimensional fluorescence microscope image analysis

An instrument combining fluorescence microscopy with Hadamard transform multiplexed imaging was designed by which a three-dimensional Hadamard transform fluorescence microscopic cell image was obtained. The image can provide useful information including, simultaneously, the apparent dimensions and the shape of the analytical sample, the content and the distribution of some species in it.     

阿达玛变换显微图象分析系统在乳腺肿瘤细胞DNA倍性分析中的应用研究

以正常人外周静脉血淋巴细胞为标准二倍体细胞，以吖啶橙为细胞DNA荧光探针，用阿达玛变换显微图象分析仪测定了六例乳腺肿瘤的细胞DNA含量（倍性），分析结果与病例学诊断结论吻合，表明该仪器可望用于乳腺癌的诊断和预后研究．研究结果还表明：乳腺肿瘤细胞DNA倍性与核面积（象素）呈显著正相关．     

Design and application of Hadamard-injectors coupled with gas and supercritical fluid sample collection systems in Hadamard transform-gas chromatography/mass spectrometry

A novel Hadamard transform-gas chromatography/mass spectrometry (HT-GC/MS) system equipped with on-line sample collection systems is described. A Hadamard-injector was successfully designed and then coupled with an on-line adsorption/desorption system for detecting volatile organic compounds (VOCs) and a supercritical fluid extraction (SFE) system, respectively, by HT-GC/MS. Six VOCs and three pesticides were used as model compounds. In the former case, an activated-charcoal trap was used to trap VOCs from the indoor air. After 10 L of indoor air had passed through the trap, the condensed components were heated and simultaneously injected into the GC column through the Hadamard-injector, based on Hadamard codes. In a second experiment, a sample of rice was spiked with three types of pesticides and the sample then extracted using a commercially available supercritical fluid extractor. After extraction, the extracted components were transferred to a holding tank and simultaneously injected into the GC column also using the Hadamard-injector. The findings show that, in both cases, the combination of on-line sample collection methods and the use of the Hadamard transform resulted in improved sensitivity and detection. Compared to the single injection used in most GC/MS systems, the signal-to-noise (S/N) ratios were substantially improved after inverse Hadamard transformation of the encoded chromatogram.     

阿达玛变换显微荧光成像在单细胞定量分析中的应用研究

本文探讨了用阿达玛变换（HT）显微荧光成像系统获得基于256灰度级图像的单细胞荧光强度和解码定标值之间的关系,将归一化的HT图像用于定量分析,建立了适合不同样品的定量分析方法.在此条件下,定量分析数据有很好的准确度和精密度;将系统用于单个乳腺肿瘤细胞的DNA定量分析,对乳腺肿瘤的良恶性及癌变程度进行判断,分析结果与病理学诊断结论一致.