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Instrument for Hadamard transform three-dimensional fluorescence microscope image analysis |
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| Authors: | Guanquan Chen Erwen Mei Wenfang Gu Xiaobin Zeng and Yun'e Zeng |
| Institution: | a Centre of Instrumental Analysis, Wuhan University, Wuhan, 430072, China b Department of Chemistry, Wuhan University, Wuhan, 430072, China |
| Abstract: | An instrument combining fluorescence microscopy with Hadamard transform multiplexed imaging was designed by which a three-dimensional Hadamard transform fluorescence microscopic cell image was obtained. The image can provide useful information including, simultaneously, the apparent dimensions and the shape of the analytical sample, the content and the distribution of some species in it. |
| Keywords: | Image analysis Fluorimetry Microscopy Hadamard transform |
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