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在碱性环境下,银(Ⅲ)配合物可与鲁米诺产生化学发光,醋酸泼尼松对该发光体系具有显著的增敏作用,据此提出了流动注射银(Ⅲ)配合物-鲁米诺化学发光体系测定醋酸泼尼松含量的方法。优化的试验条件如下:1鲁米诺溶液中氢氧化钠的浓度为0.6mol·L-1;2鲁米诺溶液的浓度为8.0×10-7 mol·L-1;3银(Ⅲ)配合物溶液中氢氧化钠的浓度为1.7mol·L-1;4银(Ⅲ)配合物溶液的浓度为5.0×10-5 mol·L-1。醋酸泼尼松的线性范围为6.0×10-8~8.0×10-5 mol·L-1,方法的检出限(3s/k)为2.9×10-9 mol·L-1。对1.0×10-6 mol·L-1醋酸泼尼松标准溶液连续测定11次,测定值的相对标准偏差为2.9%。加标回收率在100%~105%之间。 相似文献
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在碱性环境下,银(Ⅲ)配合物可与鲁米诺产生化学发光,醋酸泼尼松对该发光体系具有显著的增敏作用,据此提出了流动注射银(Ⅲ)配合物-鲁米诺化学发光体系测定醋酸泼尼松含量的方法。优化的试验条件如下:1鲁米诺溶液中氢氧化钠的浓度为0.6mol·L-1;2鲁米诺溶液的浓度为8.0×10-7 mol·L-1;3银(Ⅲ)配合物溶液中氢氧化钠的浓度为1.7mol·L-1;4银(Ⅲ)配合物溶液的浓度为5.0×10-5 mol·L-1。醋酸泼尼松的线性范围为6.0×10-8~8.0×10-5 mol·L-1,方法的检出限(3s/k)为2.9×10-9 mol·L-1。对1.0×10-6 mol·L-1醋酸泼尼松标准溶液连续测定11次,测定值的相对标准偏差为2.9%。加标回收率在100%~105%之间。 相似文献
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鲁米诺-过硫酸钠-卡托普利化学发光体系的研究 总被引:1,自引:0,他引:1
基于卡托普利对过硫酸钠鲁米诺化学发光体系的强烈抑制作用,建立起一种直接测定卡托普利的流动注射化学发光新方法。该方法灵敏、简单、快速。线性范围为5.0×10-5~1.0×10-3g·L-1,检出限为1.7×10-5g·L-1,相对标准偏差为3.2%。利用该方法对卡托普利片剂含量的测定,结果满意。 相似文献
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偶合反应流动注射化学发光测定锆的研究 总被引:1,自引:0,他引:1
基于Zr置换Co-NTA配合物中的Co(Ⅱ)和Co(Ⅱ)催化过氧化氢氧化鲁米诺产生化学发光的反应,建立了偶合反应流动注射化学发光测定锆的新方法。方法的线性范围为1×10^-10-1×10^-7g.ml^-1,检出限为3×10^-11g.ml^-1,方法用于地矿术中锆的测定,结果满意。 相似文献
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A new method for the determination of baicalin in Scutellariae Radix extract and its preparations by high performance liquid chromatography (HPLC) coupling with flow injection chemiluminescence detection (FIA‐CL) has been developed. The method was based on the chemiluminescence reaction of potassium permanganate with baicalin in nitric acid medium; the CL intensity can be enhanced by formaldehyde. In this study, the conditions of chemiluminescence and chromatography were examined, and the schematic diagram of the HPLC‐FIA‐CL analyzer was optimized. The analytes were separated on Hypersil RP‐C18 columns (100 × 4.6 mm, I.D., 5 μm) by equality elution with 47:53 (v/v) methanol‐0.3% phosphoric acid as a mobile phase at a flow rate of 0.8 mL·min?1 and a column temperature of 40 °C. Under the optimum condition, the CL intensity was proportional to the concentration of baicalin over the range of 4.10 × 10?7 ? 6.15 × 10?5mol·L?1. The limit of detection (S/N = 3) was 2.79 × 10?7mol·L?1 with the relative standard deviation 2.5% (Cs = 6.15 × 10?6 mol·L?1, n = 5). The method has been applied to the determination of baicalin in Scutellariae Radix extract and its preparations, and satisfactory results were obtained. 相似文献
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A novel peroxyoxalate chemiluminescence system has been designed for the determination of Triton X‐100 (TX‐100), in which a hydrophobic fluorescent conjugated polymer, poly[2,5‐bisnonyloxy‐1,4‐phenylene‐ethynylene‐9,10‐anthrylene] (PPEA) was employed as a fluorophor. A strong enhanced intensity of chemiluminescence (CL) was observed in the presence of TX‐100, due to the improved emission efficiency of PPEA in the presence of TX‐100. Under optimum conditions, the detection range of Triton X‐100 is between 1.0×10?7 and 1.0× 10?4 mol·L?1, with a detection limit at 6.0×10?8 mol·L?1. The relative standard deviation is 2.4% (n=6) for 1.0×10?6 mol·L?1 Triton X‐100. This method provides satisfying results in the detection of TX‐100 in nature water and biological samples with high sensitivity and wide linear range. 相似文献
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A flow-injection chemiluminescence (CL) method for the determination of melatonin based on the CL reaction of melatonin with hydrogen peroxide and sodium hypochlorite (NaOCl) in a basic alkaline solution was developed. The possible CL mechanism has been discussed, and a proposal for the reaction pathway was given that singlet oxygen was clarified to be produced in this reaction system and was responsible for the CL emission. Under the optimized conditions, the linear concentration range of application was 1.0×10^-7-2.5 × 10^-4 moloL-I with a de- tection limit of 5.0 ×10^-8 moloL-1 (S/N= 3). The relative standard deviation for eight repeated measurements of 1.0×10^-6 mol·L^-1 melatonin was 2.8%. The interferences of several important biological substances, some indole compound, cations and anions were studied. No interference was found for the anions, glucose, starch, most of cations and low concentration (less than 3.0 × 10^-6 mol·L^-1) of some biological substances and indole compound. The method was applied to the determination of melatonin in rat pineal gland and drug with satisfactory results. The sample throughput was 90 injections per hour. 相似文献
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《Analytical letters》2012,45(1-3):137-145
A sensitive flow injection chemiluminescence (CL) method is proposed for the determination of bovine serum albumin (BSA) using Copper(II)-Alizarin Red S (ARS) complex as an efficient chemiluminescent probe. The detection is based on the binding of the copper(II)-ARS complex to proteins and the catalytic activity of copper(II)-ARS in the luminol-H2O2 CL system. Under the selected conditions, the CL intensity is linear with the concentration of BSA in the range of 5.0 × 10?11 to 1.0 × 10?9 mol · L?1. The detection limit was 2.0 × 10?11 mol · L?1. The method is successfully applied to the determination of protein in urine. 相似文献
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A novel flow injection procedure has been developed for the determination of gallic acid based on the enhancement function for luminol‐AgNO3‐Ag NPs chemiluminescence (CL) system by gallic acid. The enhancement mechanism was proposed for the reinforcing effect of the gallic acid on the CL system. The UV‐vis absorption spectrum and CL emission spectrum were applied to confirm the mechanism. The method is simple, rapid and sensitive with a detection limit of 5×10?10 g·mL?1 and a linear range of 8.0×10?10–1.0×10?7 g·mL?1. The relative standard deviation (RSD) is 1.3% for eleven measurements of 5×10?8 g·mL?1 gallic acid. The method has been successfully applied to the determination of gallic acid in Chinese proprietary medicine–Jianmin Yanhou tablets and synthesized samples. 相似文献
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Luminol and hemin dual-functionalized silica nanoparticles were synthesized using a typical reverse water-in-oil microemulsion protocol. The obtained nanoparticles were further characterized by transmission electron microscopy, scanning electron microscopy, atomic absorption spectrometry, chemiluminescence, and electrochemiluminescence. The results indicated that the luminol and hemin dual-functionalized silica nanoparticles exhibited significantly higher chemiluminescence and electrochemiluminescence intensities than those of luminol functionalized silica nanoparticles due to the catalytic effect of hemin on the chemiluminescence and electrochemiluminescence of luminol. Furthermore, a simple and sensitive label-free electrochemiluminescence DNA biosensor was developed based on the chitosan modified luminol and hemin dual-functionalized silica nanoparticles and a single-stranded DNA probe. The chitosan modified luminol and hemin dual-functionalized silica nanoparticles were immobilized on the surface of an indium-doped tin oxide electrode and the single-stranded DNA probe was immobilized on the surface of the nanoparticles through electrostatic interactions between single-stranded DNA and chitosan, which allowed hybridization with the target DNA sequences. The hybridization events were evaluated by electrochemiluminescence, and only the complementary sequence formed double-stranded DNA with the DNA probe to give strong electrochemiluminescence signals. Finally, the electrochemiluminescence intensity was found to be linearly related to the concentration of the complementary sequence at concentrations from 1.0?×?10?12 to 1.0?×?10?6?mol·L?1 with a detection limit of 5.0?×?10?13?mol·L?1. 相似文献
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A highly sensitive automated sequential‐injection chemiluminescence (SIA‐CL) method for determination of glucosamine sulphate (GLS) was developed. The goal of the present work is the evaluation of the enhancement effect of the investigated drug glucosamine sulphate on the chemiluminescence reaction between luminol and H2O2 in alkaline medium of 1.0 × 10?2 mol L?1 sodium hydroxide at pH 11. The experimental conditions affecting the CL reaction such as the sequence of the reagents, concentrations, flow rate and aspirated volumes of reactants were systematically investigated and optimized. Under optimum conditions 50 μL of 1.0 × 10?3 mol L?1 luminol, 30 μL of a GLS test solution and 50 μL of 1.0 × 10?2 mol L?1 H2O2 were used and the luminescing zone was pushed into the detector at a flow rate 100 μL s?1. The proposed method recorded high sensitivity, accuracy and simplicity that could be clarified as linear concentration range 1.0‐2000 ng mL?1 with rectilinear part (r = 0.9992, n = 9) and limit of detection 0.3 ng mL?1, along with relative standard deviation 1.3%. It was found that the developed method can be used directly to determine the investigated drug GLS in its pharmaceutical dosage forms and in spiked serum and urine by diluting the samples for a 1000 fold. The obtained results were statistically analyzed and compared with those obtained by the reported method. 相似文献
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《Analytical letters》2012,45(16):3148-3157
Abstract A simple, rapid, and automated assay for hydrogen peroxide in pharmaceutical samples was developed by combining the multicommutation system with a chemiluminescence (CL) detector. The detection was performed using a spiral flow‐cell reactor made from polyethylene tubing that was positioned in front of a photodiode. It allows the rapid mixing of CL reagent and analyte and simultaneous detection of the emitted light. The chemiluminescence was based on the reaction of luminol with hydrogen peroxide catalyzed by hexacyanoferrate(III). The feasibility of the flow system was ascertained by analyzing a set of pharmaceutical samples. A linear response within the range of 2.2–210 µmol l?1 H2O2 with a LD of 1.8 µmol l?1 H2O2 and coefficient of variations smaller than 0.8% for 1.0×10?5 mol l?1 and 6.8×10?5 mol l?1 hydrogen peroxide solutions (n=10) were obtained. Reagents consumption of 90 µg of luminol and 0.7 mg of hexacyanoferrate(III) per determination and sampling rate of 200 samples per hour were also achieved. 相似文献
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A novel biosensor by electrochemically codeposited Pt nanoclusters and DNA film was constructed and applied to detection of dopamine (DA) and uric acid (UA) in the presence of high concentration ascorbic acid (AA). Scanning electron microscopy and X‐ray photoelectron spectroscopy were used for characterization. This electrode was successfully used to resolve the overlapping voltammetric response of DA, UA and AA into three well‐defined peaks with a large anodic peak difference (ΔEpa) of about 184 mV for DA and 324 mV for UA. The catalytic peak current obtained from differential pulse voltammetry was linearly dependent on the DA concentration from 1.1× 10?7 to 3.8×10?5 mol·L?1 with a detection limit of 3.6×10?8 mol·L?1 (S/N=3) and on the UA concentration from 3.0×10?7 to 5.7×10?5 mol·L?1 with a detection limit of 1.0×10?7 mol·L?1 with coexistence of 1.0×10?3 mol·L?1 AA. The modified electrode shows good sensitivity and selectivity. 相似文献
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A simple, rapid and accurate high performance liquid chromatographic (HPLC) technique coupled with chemiluminescence (CL) detection was developed for the simultaneous determination of epinephrine (E), noradrenaline (NA) and dopamine (DA). It was based on the analyte enhancement effect on the CL reaction between luminol and potassium ferricyanide. The effects of various parameters, such as potassium ferricyanide concentration, luminol concentration, pH value and component of the mobile phase on chromatographic behaviors of the analytes (E, NA and DA) were investigated. The separation was carded out on C18 column using the mobile phase of 0.01 mol/L potassium hydrogen phthalate solution and methanol (92 : 8, V/V). Under the optimum condi- tions, E, NA and DA showed good linear relationships in the range of 1 × 10^-8 -5 × 10^-6, 5.0× 10^-9 -1.0× 10^-6 and 5.0×10^-9-1.0× 10^-6 g]mL respectively. The detection limits for E, NA and DA were 4.0×10^-9, 1.0× 10^-9 and 8.0 × 10^-10 g/mL. The proposed method has been applied successfully to the analysis of E, NA and DA in human serum samples. 相似文献