非水溶液毛细管电泳

评述了非水溶液毛细管电泳的应用及发展，对非水溶液毛细管电泳的原理及应用情况作了简要叙述，有机试剂在毛细管电泳中的加入，扩大了分析物质的范围，以纯有机试剂作电泳介质，具有许多水溶液毛细管电不能比拟的优点。     

毛细管电泳的原理及应用（第二讲）毛细管电泳的原理及应用

毛细管电泳的原理及应用（第二讲）毛细管电泳的原理及应用罗国安，王义明（清华大学生命科学与工程研究院，清华大学化学系北京１０００８４）１概述毛细管电泳（ＣＥ）除了比其它色谱分离分析方法具有效率更高、速度更快、样品和试剂耗量更少、应用面同样广泛等优点外，...     

#电渗泵流动注射系统测定水中氟

对电渗泵的原理及其在流动注射系统中的应用特点作了详细讨论，并用氟试剂分光光度法测定了自来水中氟化物含量。     

高密度脂蛋白胆固醇的定量测定方法、试剂及试剂盒

本发明提供了一种定量测定血清样品中高密度脂蛋白胆固醇含量的试剂，该试剂由分别放置的试剂Ⅰ和试剂Ⅱ组成，其中，所述试剂Ⅰ含有多聚阴离子化合物、二价阳离子盐及分散型表面活性剂；所述试剂Ⅱ含有苯乙烯基苯酚聚氧乙烯（16）醚。此外，本发明还提供了一种装有本发明试剂Ⅰ和试剂Ⅱ的试剂盒，以及一种测定高密度脂蛋白胆固醇含量的方法。     

基准试剂纯度定值研究

本文介绍了基准试剂纯度定值装置的工作原理,研究制作了用于反映库仑滴定终点的终点指示器,并对基准试剂的纯度分析方法进行了研究,使定值精度达到了国内外先进水平。     

毛细管电泳的原理及应用（第二讲）毛细管电泳的原理及应用

 毛细管电泳的原理及应用（第二讲）毛细管电泳的原理及应用罗国安，王义明（清华大学生命科学与工程研究院，清华大学化学系北京１０００８４）１概述毛细管电泳（ＣＥ）除了比其它色谱分离分析方法具有效率更高、速度更快、样品和试剂耗量更少、应用面同样广泛等优点外，其仪器结构也比高效液相色谱（ＨＰＬＣ）简单。ＣＥ只需高压直流电源、进样装置、毛细管和检测器。前三个部件均易实现，困难之处在于检测器。     

水杨基萤光酮-溴化十六烷基三甲铵荧光猝灭法测定微量铝的研究

近几年,用各种新型试剂荧光光度法测定铝的文献报道颇多,其原理多采用铝与某一荧光试剂生成产生荧光的配合物进行测定。但利用铝(Al)-水杨基萤光酮(SAF)-溴化十六烷基三甲铵(CTMAB)三元配合物的形成,使试剂(SAF-CTMAB)荧光猝灭而测定铝的方法尚未见报道。笔者发现,在汞弧光激发下,SAF产生波长为515nm的较强荧光,引入     

科学出版社化学类图书精品推荐

分子探针与检测试剂 (“十五”国家重点图书出版规划项目 )张华山等编著　　 2 0 0 2年 8月出版　　定价 :4 5元本书内容丰富 ,取材新颖 ,充分反映了有机试剂基础理论研究的新进展和新成果。本书共分 12章 ,较全面系统地阐述分析化学及生物分析中的有机试剂、分子及离子荧光探针。内容包括 :光度分析中的有机显色剂、荧光试剂、发光试剂、超分子显色剂的作用原理及应用 ;离子荧光探针、分子荧光探针及其生物活性物质的测定和生命科学研究中的应用 ;高效液相色谱和气相色谱中的衍生试剂及应用。本书可供化学化工、生物技术、冶金、地质、农业…     

高效液相色谱在生物医药研究中的应用第六讲高效液相色谱分离光学异物体（下）

2.手性流动相法 手性流动相法亦称乒性添加剂法。优点是拆分化合物不需以手性试剂衍生,也不需要价格昂贵的CSP,而是将手性试剂加到流动相中,使用常规的正相或反相柱分离,由于方法简便,引起广泛的注意。作用原理有两种可能:(1)溶质在流动相中与手性试剂形成非对映络合物,因而在固定相     

折射＼率对流动注射峰形影响的研究

本文利用折射定律、菲涅耳公式和流体力学原理研究不同浓度乙醇溶液为试剂或载流的单流路体系中，正向流动注射与反向流动注射的信号峰形，对每个正、负峰高度和位置进行解释。给出不同相界面曲面方程，计算不同条件下反射率，反射光和折射光被流通池壁吸收时的径向半径及发生全反射时的径向半径，从理论上计算各种条件下的吸光度。     

Simulation of the Long Term Behaviour of Plastics Components

Summary: This paper presents a method to model the mechanical behavior of polymers over a wide time- and load-range by means of finite element analyses. The method includes a material model as well as the determination of material parameters to calibrate the material model. As a special feature of this method the model is calibrated only by using creep data that are commonly available in material data bases. So the procedure improves the simulation of the long time behavior of plastic-components without an additional experimental effort. In combination with time-temperature-superposition principle, the temperature dependency of the long term behavior is represented, too. The simulation results are validated by creep experiments on an example part.     

Substoichiometric method in the simple radiometric analysis

The substoichiometric method is applied to simple radiometric analysis. Two methods-the standard reagent method and the standard sample method-are proposed. The validity of the principle of the methods is verified experimentally in the determination of silver by the precipitation method, or of zinc by the ion-exchange or solvent-extraction method. The proposed methods are simple and rapid compared with the conventional superstoichiometric method. The University has moved and changed its name and address as lollows: Institute of Chemistry, The University of Tsukuba 300-31 Sakura-mura, Ibaraki Japan.     

Some possibilities for the plutonium content determination in an irradiated nuclear fuel

This paper describes the results of the methods used for the plutonium content determination in an irradiated nuclear fuel from the first Czechoslovak atomic power station A1. The main attention was paid to the following methods: mass-spectrometric isotope dilution method, radiometric method and correlation dependence method based on the analysis of the burnt fuel. The principle and the accuracy of the individual methods are discussed.     

Determination of the kinetic parameters from non-isothermal measurements with a general temperature program

This paper deals with a new method for the evaluation of the kinetic parameters from thermogravimetric measurements with a general temperature program. The procedure assumes the use of a computer or calculator. In principle, it is an integral method with two variants. The kinetic parameters can be determined from a single and/or from two general temperature programs. This method is free of the shortcomings that the existing method has, i.e. the self-heating and/or self-cooling, resulting in errors in measurements and the limitation of the weight of sample. The two variants of the submitted method have been tested by evaluation of the experimental data of the thermal decomposition of CaCO₂.     

Mathematical model for the analytical signal of an herbicide sensor based on the reaction centre of Rhodobacter sphaeroides

This paper introduces a mathematical model which makes it possible both to determine the concentration of photosynthetic herbicides and to obtain a quantitative parameter in order to compare their activity using a previously described sensing system. The working principle involves the changes in absorption properties at 860 nm of the reaction centre (RC) isolated from the bacteria Rhodobacter sphaeroides when photosynthetic herbicides are present. The method has been used for the determination and activity comparison of five photosynthetic herbicides: diuron, atrazine, terbutryn, terbuthylazine and simazine. Detection limits obtained were 2.2, 0.75, 0.046, 0.25, and 1.4 μM, respectively. The resulting order for the different herbicides according to their action on RC was: terbutryn > terbuthylazine > atrazine > simazine > diuron.     

Diels-Alder反应轨道作用情况研究

根据三轨道作用法重点讨论了Diels-Alder反应中反应物轨道演变成产物轨道的过程, 演变结果与轨道对称守恒原理的结果基本相同. 量子化学计算证实了演变过程的合理性, 这表明在解释D-A反应方面轨道对称守恒原理比前线轨道理论更加合理可靠.     

薄层流动固定电位时安法研究

薄层流动固定电位时安法,系在薄的小体积电解池中,用金盘作工作阴极,银-氯化银作阳极,控制于极谱极限电流电位,测定试样溶液由流动状态转换至静止状态时的时安电量。叙述了定量分析原理,电量-浓度方程式,实验方法,克服了电容电流及电极性能不稳定等干扰。灵敏度提高至10^-7M,噪音低,分辨力高,稳定,再现性好。与阳极解析伏安法相结合,优点显著。     

A minimum principle for molecular systems allowing the use of discontinuous wave functions

In this paper the minimum principle proposed for atomic systems by Hall, Hyslop and Rees [1] is generalized to molecules. It is shown that this generalization retains the advantage of admitting the use of a larger class of trial wave functions, for example those with discontinuities, than is possible in the usual minimum energy principle. The further advantage that the upper bounds obtained by this treatment are always at least as good as those of the Rayleigh-Ritz method is also preserved. The theory is applied to the H ion, potential energy curves are obtained for various “cut-off” wave functions, and the equilibrium internuclear distance is calculated. The optimization of the “cut-off” region so that the upper bound is minimized is also discussed.     

测定可见光区分光光度系组成的新方法

本文报道一种测定二元分光光度系组成的新方法,原理新颖,为作者首创,采用双波长分光光度技术消除显色产物的影响,直接测定与显色剂平衡浓度相对应的吸光度,通过简单的计算即可求得产物组成。     

一种新型磁天平在配合物磁化率测定中的应用

经典物理化学实验"磁化率测定实验"中,传统方法是使用Gouy磁天平,仪器笨重且操作复杂。本文提供一种新型台式磁天平,用于配合物磁化率的测定。文中通过测量三种亚铁化合物的磁化率,介绍该磁天平的基本原理、使用方法、操作步骤和数据分析。通过比较测量值和标准值,得出该仪器的测量误差小、精确度高,适用于实验教学和科学研究。