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亚波长尺寸纵向偏振光束在粒子加速,单个分子偶极矩测量,二次谐波成像和纵向偏振共焦显微镜等方面有非常广泛的应用.本文提出径向调制的Bessel-Gaussian光束模型,经高数值孔径显微物镜聚焦可以获得高纯度的纵向偏振光束.利用矢量衍射理论数值模拟了焦点附近光强分布、磁场分布和能流密度空间分布.结果表明光斑半高全宽度可以达到0.4λ.该方法无需环形孔径或环形相位滤波器,光能利用率高,分辨率好,达到改进各种应用的效果.
关键词:
Bessel-Gaussian光束
纵向偏振光束
聚焦
亚波长 相似文献
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采用附加探测光声子耗尽法来实现超衍射极限相干反斯托克斯拉曼散射显微成像. 此方法引入一束环形分布的附加探测光来消耗点扩展函数周边的相干声子, 实现点扩展函数的改造, 从而达到超越衍射极限的空间分辨率. 为了获得更高的空间分辨率和更佳的相位匹配条件, 通常需采用高数值孔径物镜对抽运光、斯托克斯光和探测光进行聚焦, 此时标量衍射理论不再成立. 基于矢量衍射理论, 分析了线偏振光、圆偏振光先后经过螺旋相位片和高数值孔径物镜后的光强分布, 结果表明: 圆偏振光在高数值孔径物镜后焦平面的光强分布呈中心对称状, 较线偏振环形光更适合作为附加探测光. 此外, 采用全量子理论分析了附加探测光声子耗尽法. 结果表明: 当附加探测光与探测光强度比为80时, 成像系统的横向空间分辨率可以达到45 nm; 继续提高附加探测光强度, 空间分辨将进一步提高. 相似文献
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为解决普通生物显微物镜视场小、场曲和色差严重的问题,本文利用光学软件Code V设计了一款20×平场复消色差显微物镜,通过合理的结构优化、光焦度分配及材料选择,使该物镜具有大视场、高数值孔径、平场复消色差的特点。设计分析结果表明,该物镜平场数为0.11 μm,最大焦移量为0.5 μm,满足显微物镜国际标准的规定;光学传递函数(MTF)曲线与理论衍射极限接近,达到指标要求,该物镜在可见光范围内实现了平场复消色差设计。公差分析结果表明RMS波前差劣化至0.24λ,能够满足加工和实际应用要求。 相似文献
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《光学学报》2017,(3)
受激发射损耗显微技术(STED)作为一种远场超分辨显微成像技术,具有几十纳米甚至几纳米的空间分辨率,是细胞生物学等研究领域的重要成像工具。圆环形空心损耗光在物镜焦点附近的光场强度分布对STED空间分辨率起决定性作用。在高数值孔径物镜聚焦下,光场的偏振态会对聚焦光场的强度分布产生显著的影响,此外,显微系统的轴外像差会严重破坏空心损耗光焦斑的中心对称性。基于矢量衍射理论,理论模拟了在高数值孔径物镜聚焦条件下,入射涡旋光的偏振态和光学系统中的彗差和像散对空心损耗光焦场强度分布的影响。实验上使用纯相位型空间光调制器来校准光学系统相差,优化变形的损耗光,利用纳米探针扫描焦点区域,测量了其焦场强度分布。测量结果与由矢量稍微理论观测的结果一致。 相似文献
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《光学学报》2021,41(4):97-107
傅里叶叠层显微成像(FPM)利用LED阵列角度变化的光照来克服低数值孔径物镜的分辨率限制。在传统的FPM系统中,LED阵列的位置误差将会给图像重建过程带来严重影响。因此准确校正LED阵列的位置对于提高重建图像质量至关重要。为了解决这一问题,提出一种基于遗传退火优化算法的位置校正方法。首先分析LED阵列、样品及物镜数值孔径的相对位置给入射波矢量带来的影响;接着采用遗传退火优化算法对LED阵列的误差位置估计全局误差参数;最后在重建过程中利用全局误差参数快速、准确地对LED阵列位置进行校正。仿真结果和实验结果表明,所提方法能显著提高重建图像的质量。 相似文献
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The annular binary pupil filters consist of two transparent annuli obstructed by an annular mask. The optical transfer functions for them are analytically derived under paraxial approximation, and three- and two- as well as one-dimensional numerical plots are presented. It is shown that by changing the parameters of transparent regions it is possible to improve the axial resolution whilst the transverse resolution remains unchanged, compared with an annular lens. Results indicate, as is well known, that this kind of filters can be applied to three-dimensional confocal microscopy. 相似文献
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In this paper, a new method for designing three-zone optical pupil filter is presented. The phase-only optical pupil filter and the amplitude-only optical pupil filters were designed. The first kind of pupil for optical data storage can increase the transverse resolution. The second kind of pupil filter can increase the axial and transverse resolution at the same time, which is applicable in three-dimension imaging in confocal microscopy. 相似文献
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提出一种新的具有高空间分辨力的整形环形光式差动共焦测量方法。该方法通过整形环形光式共焦测量法和锐化爱里斑主瓣,改善系统横向分辨力;通过差动共焦测量法改善系统的轴向分辨力,最终达到提高系统空间分辨能力的目的。理论分析和实验表明:整形环形光内孔归一化半径ε越大,横向分辨力改善越明显,量程扩展范围越宽;当入射光波长λ=632.8nm,物镜数值孔径取NA=0.85,ε=0.5时,该系统的横向分辨力优于0.2μm,轴向分辨力优于2nm。该方法为光触针测量系统空间分辨力的提高提供了1种新的方法,可广泛应用于超精密三维微细结构工件的超精密测量。 相似文献
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We design three-zone annular filters to be applied to optical storage system. The designed filters extend the depth of focus and realize transverse superresolution simultaneously, which will improve the performance of optical storage system greatly. And we propose two feasible schemes to improve imaging resolution of three-dimensional imaging system. One scheme depends on a complex filter formed by cascading of a three-zone phase filter and a three-zone amplitude filter. The complex filter converge the optimized transverse superresolution and the optimized axial superresolution of two different filters onto a single filter. It can improve the three-dimensional imaging performances greatly. Another scheme depends on a single three-zone complex filter. We propose a three-zone complex filter with phase shift 0.8π, which presents bigger design margin, better imaging quality and stronger three-dimensional superresolution capability. 相似文献
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A shaped annular beam tri-heterodyne confocal microscope with good anti-environmental interference capability 下载免费PDF全文
A shaped annular beam tri-heterodyne confocal microscope has been proposed
to improve the anti-environmental interference capability and the resolution
of a confocal microscope. It simultaneously detects far-, on-, and
near-focus signals with given phase differences by dividing the measured
light path of the confocal microscope into three sub-paths (signals).
Pair-wise real-time heterodyne subtraction of the three signals is used to
improve the anti-environmental interference capability, axial resolution,
and linearity; and a shaped annular beam super-resolution technique is used
to improve lateral resolution. Theoretical analyses and preliminary
experiments indicate that an axial resolution of about 1 nm can be achieved
with a shaped annular beam tri-heterodyne confocal microscope and its
lateral resolution can be better than 0.2 $\mu $m for $\lambda =632.8$~nm,
the numerical aperture of the lens of the microscope is NA $=0.85$, and the
normalized radius $\varepsilon =0.5$. 相似文献
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用矢量衍射方法分析了线偏振光入射到带有三环位相型光瞳滤波器的高数值孔径物镜时,焦点的轴向和横向光强分布.数值模拟结果表明,高数值孔径物镜聚焦时需同时考虑光强的轴向和横向分布.通过加入三环位相光瞳滤波器,在实现横向超分辨的同时实现了光学系统轴向焦深扩展和轴向光强分布平坦化,并且位相调制深度变化时,会出现轴向焦移现象.对三环位相光瞳滤波器结构进行了优化,得出了优化结果.
关键词:
光学超分辨
扩展焦深
位相型光瞳滤波器
矢量衍射方法 相似文献
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利用matlab优化工具箱,采用非线性规划,对三区振幅型(透过率为1-0-1)和三区位相型(相位角为π-0-π)光瞳滤波器进行三维超分辨优化设计,建立了优化模型,经计算机多次迭代运算,改变斯特尔比s值,给出了优化结果.结果显示:所设计的滤波器较好地实现了横向和轴向三维超分辨,且轴向超分辨能力优于横向;相同斯特尔比下,位相型三维超分辨能力优于振幅型,并且这两种滤波器结构简单,容易实现. 相似文献
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The performance of pupil filters consisting of two zones each of constant complex amplitude transmittance is investigated. For filters where the transmittance is real, different classes of potentially useful filter are identified and optimized. These include leaky filters with an inner zone of low amplitude transmittance, pure phase filters with phase change of π, and equal area filters. The first of these minimizes the relative power in the outer rings for a given axial resolution, the second maximizes the Strehl ratio for a given transverse resolution, and the third minimizes the relative power in the outer rings for a given transverse resolution. Complex filters can give an axially shifted maximum in intensity: the performance parameters calculated relative to the true focus are investigated for some different classes of filter, but filters with phase change not equal to π are found to give inferior performance to the real value filters. 相似文献