共查询到18条相似文献,搜索用时 156 毫秒
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二蕊荷莲豆环肽B的NMR应用研究 总被引:6,自引:1,他引:5
植物环肽的1H 和13C NMR图谱, 由于各种氨基酸自旋系统质子和碳的化学位移非常接近,谱峰高度重叠,结构解析比较困难. 文中以二蕊荷莲豆环肽B为例讨论了
现代2D NMR新技术,在植物环肽结构解析中的应用. HMQC-TOCSY图谱在氢谱方向和碳谱方向分别提供每一个氨基酸自旋系统内的氢和除季碳外碳的全相关信息,从而将每个氨基酸残基的NMR信号相互区分开来;结合1H-1H COSY 和 HMQC或HSQC图谱,就可以准确归属每个氨基酸的氢和碳的化学位移. 氨基酸残基之间的连接顺序可用HMBC、NOESY或ROESY图谱获得. 相似文献
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二维核磁共振谱测定植物新环肽-太子参环肽A和B中氨基酸序列 总被引:2,自引:0,他引:2
在植物新环肽[太子参环肽A(HA)和B(HB)]的结构研究中,应用COLOC谱较成功地解决了其氨基酸序列。结果表明当J值选择在6Hz-15Hz时,COLOC谱能满意地给出氮上含有活泼氢的氨基酸序列。 相似文献
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核自旋单重态是一种特殊的自旋状态,其寿命远长于相应自旋的横向和纵向弛豫时间,能够被用于研究分子的慢扩散、慢运动、特征信号选择等过程.目前单重态的研究主要集中于孤立的两自旋体系.而本文以N-乙酰基天冬氨酸(NAA)分子中由亚甲基和次甲基的三个氢原子核构成的三自旋体系为研究对象,将亚甲基中的两个氢核制备成单重态.利用优化控制和数值计算方法,分别设计了包含和不包含次甲基氢核耦合的单重态制备脉冲,结果发现,不考虑次甲基氢耦合设计的优化脉冲,其在实际三自旋体系中的单重态制备效率会显著下降.另外,我们以单重态为起点,实现了针对次甲基和亚甲基的信号选择COSY谱和NOESY谱,结果表明基于单重态的二维谱能够有效避免谱峰重叠现象,提高谱图分辨率,并有助于提高分子结构解析的准确性. 相似文献
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本刊1988年第三、四期“原子核高自旋超形变的研究”一文对高自旋超形变谱学作了初步介绍。本文综合分析近两年来该领域的新进展。两年来,A~190Hg-Pb 超形变区的确认;A~150区奇质子核及更多超形变核的发现;A~130—140区奇质子超形变核和Sm 超形变核的研究;同一个核中多重超形变带的发现;各超形变区超形变带丰富的谱学现象,超形变组态-高j 侵入轨道的不同占据对超形变带谱学性质的成功描述,及对理论模型的检验和修正;A~190和A~150区相邻(同位素和同中素)核中超形变带一系列性质的极端相似—超形变带中内禀自旋排列和质子激发的发现;超形变、高自旋下的对关联研究;超形变带退激馈入正常形变带的时间延迟的实验测定;超形变退激机制的研究;应用于Hg-Pb区超形变带自旋指定的一种有效方法等,都是原子核超形变研究的主要成果。此外,锕系区超形变核中与同质异能裂变竞争的、由位能面第二极小向第一极小退激的“返回”γ衰变(E1跃迁)的实验测定,是原子核在零自旋和低自旋下超形变研究的一个重要进展。 相似文献
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A. Lichnerowicz 《Letters in Mathematical Physics》1989,18(4):333-345
Two interesting conformal invariants which are constant on the manifold are given for twistor-spinors on a spin manifold following the notion of a twistor-spinor associated to a twisted spin bundle. For a twisted spin bundle corresponding to a flat Hermitian vector bundle, the associated twistor-spinors admit the same conformal invariants.An analysis is made of the twistor-spinors given by
, where f is a complex-valued function. There is only one case where is not a Killing spinor. An example is given of a compact spin manifold for which the situation is realized. 相似文献
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3D HCCH(3)-TOCSY for resonance assignment of methyl-containing side chains in (13)C-labeled proteins
Uhrín D Uhrínová S Leadbeater C Nairn J Price NC Barlow PN 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2000,142(2):288-293
Two 3D experiments, (H)CCH(3)-TOCSY and H(C)CH(3)-TOCSY, are proposed for resonance assignment of methyl-containing amino acid side chains. After the initial proton-carbon INEPT step, during which either carbon or proton chemical shift labeling is achieved (t(1)), the magnetization is spread along the amino acid side chains by a carbon spin lock. The chemical shifts of methyl carbons are labeled (t(2)) during the following constant time interval. Finally the magnetization is transferred, in a reversed INEPT step, to methyl protons for detection (t(3)). The proposed experiments are characterized by high digital resolution in the methyl carbon dimension (t(2max) = 28.6 ms), optimum sensitivity due to the use of proton decoupling during the long constant time interval, and an optional removal of CH(2), or CH(2) and CH, resonances from the F(2)F(3) planes. The building blocks used in these experiments can be implemented in a range of heteronuclear experiments focusing on methyl resonances in proteins. The techniques are illustrated using a (15)N, (13)C-labeled E93D mutant of Schizosacharomyces pombe phosphoglycerate mutase (23.7 kDa). 相似文献
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在前文工作的基础上,结合MNDO/EHMO分子轨道方法和自然杂化轨道方法,具体计算了CC键和CP键的核自旋偶合常数.计算结果表明,1JCC和1JCP主要由成键原子的轨道杂化作用和键极性这两种结构因素所决定.为从简单价键理论角度解释和计算1JCC和1JCP值提供了简便直观的方法. 相似文献
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利用CPMG自旋回波技术,通过一连串180°脉冲抑制辐射阻尼效应,让磁化矢量经过横向弛豫T2过程大幅度衰减后再检测回波信号的线宽,该方法可直接获得有效横向弛豫时间T*2,并可在CPMG测试T2值的同时进行. 另外,当饱和恢复法用于估计T*2值时,信号检测必须使用小角度脉冲.所有结果已进行了实验验证. 相似文献
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Bhaskar Dutta Teruki Kamon P. Ko Jinmian Li 《The European Physical Journal C - Particles and Fields》2018,78(7):595
We study the discovery and discriminating prospects of the Higgs portal dark matter (DM) models for scalar, fermion and vector DM and their extensions in proton–proton (pp) collisions. The \(t\bar{t}+\)DM associated production in dileptonic final states is considered, in which the stransverse mass of two leptons is found to be effective in suppressing the Standard Model backgrounds along with the missing transverse energy and the angle between two leptons. The distributions of missing transverse energy and polar angle between two leptons are used for a discrimination of the spin nature of DM. For the proposed benchmark points, the discovery/exclusion can be made with an integrated luminosity less than 1 ab\(^{-1}\) given a 1% systematic uncertainty, while the spin discrimination require integrated luminosity of a few O(10) ab\(^{-1}\) given a 0.5% systematic uncertainty. The DM phenomenology is also discussed. A consistent DM candidate can be obtained either by extending our model where the Higgs portal couples to excited dark states that decay into DM, or modifying the coupling form into pseudoscalar. 相似文献
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M. Altenbuchinger Ph. Hägler W. Weise E. M. Henley 《The European Physical Journal A - Hadrons and Nuclei》2011,47(11):140
The question how the spin of the nucleon is distributed among its quark and gluon constituents is still a subject of intense
investigations. Lattice QCD has progressed to provide information about spin fractions and orbital angular momentum contributions
for up and down quarks in the proton, at a typical scale m2 \mu^{2}_{} ∼ 4 GeV2 . On the other hand, chiral quark models have traditionally been used for orientation at low momentum scales. In the comparison
of such model calculations with experiment or lattice QCD, fixing the model scale and the treatment of scale evolution are
essential. In this paper, we present a refined model calculation and a QCD evolution of lattice results up to next-to-next-to-leading
order. We compare this approach with the Myhrer-Thomas scenario for resolving the proton spin puzzle. 相似文献
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The technique of multiple-quantum J-resolved NMR spectroscopy (MQ-JRES) is introduced and applied to the spin system SI(3)-M (such as in the example given here, the (13)CH(3)-(12)CH in alanine). The SI(3) spin system was excited to its highest quantum state (8S(y)I(x)I(y)I(y)), which consists of four coherences: quadruple quantum of (3I + S), double quantum of (3I - S), double quantum of (I + S), and zero quantum of (I - S). In the MQ spectrum generated from the projection onto the F(1) dimension, the resonances of the different multiple-quantum coherences are resolved by their coupling constants to the remote spin (M). The absorptive lineshapes in both F(1) and F(2) dimensions enable accurate measurements of transverse relaxation rates, and both amplitude and relative signs of the long-range coupling constants are to be derived from either frequency or time domain data. The selective detection of MQ-JRES spectra of the individual MQ coherences using either phase cycling or pulsed field gradients is presented. 相似文献