Complex-amplitude-based phase unwrapping method for digital holographic microscopy

A complex-amplitude-based phase unwrapping approach for digital holographic microscopy is proposed in this paper. A quality map is derived directly from the reconstructed complex amplitude distribution of object wave to evaluate noise influence and phase reliability in the wrapped phase image. Quality-guided phase unwrapping algorithm is then implemented with the quality map to retrieve continuous phase profile. Unwrapping errors caused by unreliable phase data are successfully suppressed. The effectiveness of this method is demonstrated with simulation and experimental results.     

Automatic focusing in digital holography and its application to stretched holograms

The searching and recovering of the correct reconstruction distance in digital holography (DH) can be a cumbersome and subjective procedure. Here we report on an algorithm for automatically estimating the in-focus image and recovering the correct reconstruction distance for speckle holograms. We have tested the approach in determining the reconstruction distances of stretched digital holograms. Stretching a hologram with a variable elongation parameter makes it possible to change the in-focus distance of the reconstructed image. In this way, the proposed algorithm can be verified at different distances by dispensing the recording of different holograms. Experimental results are shown with the aim of demonstrating the usefulness of the proposed method, and a comparative analysis has been performed with respect to other existing algorithms developed for DH.     

Green's formulation for robust phase unwrapping in digital holography

We present, for the first time to the best of our knowledge, a phase unwrapping method based on an algorithm which makes use of Green's first identity. This method aims at recovering the correct phase information encoded in a fringe pattern after digital holography (DH) numerical processing. DH provides a quantitative measurement of the three-dimensional surface profile of objects. The information about the profile can be obtained in principle from the phase-map. However, the measured phase-map provides the actual phase values wrapped mod.2π so that an unwrapping process is required in order to reconstruct the object profile.     

基于Hilbert变换实现数字全息高精度相位重建

为了提高数字全息相位重建精度,研究了基于Hilbert变换重建相位的理论和方法,并通过像面数字全息实验对该方法的有效性进行了验证,同时与数字全息常规重建方法得到的结果进行了比较.结果表明:Hilbert变换本身具有消除直流项的作用,对利用频域滤波滤除零级谱后的全息图进行Hilbert变换,能够彻底消除零级衍射项的干扰,从而提高相位重建精度.与常规重建结果相比,基于Hilbert变换的相位重建结果的标准偏差降低了14.0%.本文结果对提高数字全息相位重建精度具有重要的指导意义.     

像面数字全息的重建相位误差分析和改善

像面数字全息是数字全息技术中常用的测量和成像方式,它通常采用离散傅里叶变换和频率滤波的方法进行物光波的重建.本文讨论了这些算法对重建相位的影响.首先分析了频谱泄露对于相位误差的影响,结果表明当采样周期为整数时,重建相位误差很小,因此具有极高的相位重建精度;而当不满足整周期采样时,相位重建误差有了明显的增加.为了改善频谱泄露所引起的相位误差,采用Hanning函数对数字全息图进行了预处理,结果表明Hanning窗的加入能够有效地提高重建相位的准确程度.     

显微数字全息中的3维信息重建

通过理论分析和模拟验证,研究了基于横向剪切的数字全息相位重建方法,分析并指明了详细的重建过程,提出了利用平坦区域相位数据进行线性拟合,从而获得线性相位畸变系数的方法,并指出对原始包裹相位图进行1维相位展开是横向剪切法重建数字全息相位信息的前提。对无噪声及含有噪声的全息图进行了数值重建,结果表明:对于弱噪声干扰的全息图,该方法很有效;而对于较强噪声干扰的全息图,采用中值滤波方法对原始相位图进行滤波后再重建,并对重建的相位图再次进行中值滤波,可以得到高质量的再现像;减小再现像平面抽样间隔,使剪切相位图中相邻的两个像元之间相位差的最大值小于2π,才可以获得正确的相位重建。     

Numerical reconstruction and twin-image suppression using an off-axis Fresnel digital hologram

In this work we proposed a novel approach for the numerical reconstruction of digital holography that uses only an off-axis Fresnel digital hologram and can easily be carrying out in a personal computer. From the presented off-axis holographic principle, the reference wave accompanies to the numerically reconstructed image, also makes the separation to the zero-order and twin images. Therefore, the concept can be applied to suppress the blurring of numerical reconstruction by adding the extra reconstruction plane and the formularized numerically reference wave in the reconstruction procedure. As expected, the blurring images can be separated out and removed from the reconstructed image. Then, by performing the back propagating to the exact reconstruction plane, one can obtain the pure object image without the blurring. In contrast to the temporal phase-shifting digital holographic-based scheme that employs multiple exposures the technique can be more effectively and easily overcome the blurring problem, also provide the practical feasibility in the digital holographic application. PACS 42.40.-I; 42.30.Wb; 42.40.Ht     

双Lloyd镜数字全息显微测量术

提出了一种双Lloyd镜数字全息显微测量技术.其基本思想是将Lloyd镜的共光束自干涉特性与双波长光学相位解包裹方法相结合,使用两个Lloyd镜调节参考光与物光夹角以形成共光束自干涉,从而获得一张双波长复合全息图,再通过角谱法再现得到每个波长对应的包裹相位,利用两波长的相位差求得解包裹后的相位图和三维高度分布图.采用532和632 nm两波长记录全息图,通过数值再现重构被测物体的振幅和相位信息.与标定值相比,实验值的误差小于5%,证明了该方法的有效性.     

数字全息波前重建中的像平面滤波技术研究

基于数字全息图的频谱平面滤波及像平面滤波技术,最近出现两种可变放大率的物光波前重建方法.为适应数字全息精细检测的要求,对两种滤波方法进行了研究,导出让局部重建图像布满重建平面的表达式.研究结果表明,像平面滤波技术优于频谱平面滤波技术,基于像平面滤波技术的重建方法能够按照需要的放大率高质量地重建局部物光场.给出彩色数字全...     

Autofocusing of digital holographic microscopy based on off-axis illuminations

An auto-focusing method for digital holographic microscopy has been proposed by employing two off-axis illumination beams. When specimens are illuminated by two plane waves in different directions, it is found that the farther the reconstruction plane is from the image plane, the wider the two reconstructed images are separated from each other. Thus, the image plane can be determinated by finding the minimum of the variation between the two reconstructed object waves on both the amplitude and phase distributions. The feasibility of the proposed method is demonstrated by the corresponding simulation and experiment.     

Methods of Fourier optics in digital holographic microscopy

In this paper, processing methods of Fourier optics implemented in a digital holographic microscopy system are presented. The proposed methodology is based on the possibility of the digital holography in carrying out the whole reconstruction of the recorded wave front and consequently, the determination of the phase and intensity distribution in any arbitrary plane located between the object and the recording plane. In this way, in digital holographic microscopy the field produced by the objective lens can be reconstructed along its propagation, allowing the reconstruction of the back focal plane of the lens, so that the complex amplitudes of the Fraunhofer diffraction, or equivalently the Fourier transform, of the light distribution across the object can be known. The manipulation of Fourier transform plane makes possible the design of digital methods of optical processing and image analysis. The proposed method has a great practical utility and represents a powerful tool in image analysis and data processing. The theoretical aspects of the method are presented, and its validity has been demonstrated using computer generated holograms and images simulations of microscopic objects.     

Controlling image size as a function of distance and wavelength in Fresnel-transform reconstruction of digital holograms

A method for controlling the size of amplitude and phase images reconstructed from digital holograms by the Fresnel-transform method is proposed and demonstrated. The method can provide a constant reconstruction pixel width in the reconstructed image plane, independent of the recording and reconstruction distance. The proposed method makes it possible to maintain the size of an object for a sequence of digital holograms recorded at different distances and, therefore, to subtract phase maps for an object recorded at different distances. Furthermore, the method solves the problem of superimposition in multiwavelength digital holography for color display and holographic interferometry applications.     

Twin-image problem in digital holography—a survey(Invited Paper)

Zero-order and twin images are a serious obstacle in achieving a high-quality output in in-line digital holography （DH）. They decrease the useful bandwidth of the off-axis DH. Over the years the twin image removal problem was approached both by instrumental and numerical means. The paper provides an extended survey of the proposed solutions with their pros and cons as a guide for further advance in this field. Processing of a single spatial carrier fringe pattern involves spatial filtering in the frequency domain, spatial phase-shifting （PS） or wavelet transform. A point source digital holographic microscopy （DHM）, introduction of calibration measurements or various modifications of PS technique are instrumental solutions to the twin image problem for in-line DH. Numerical solutions to the same problem include iterative and non-iterative approaches, diffraction-based and inverse problem solutions, reconstruction of purely real or phase objects and of complex objects, reconstruction of plane and volume objects. Elimination only of the zero-order image relies on non-linear filtering or additional calibration measurements.     

基于滤波成像的大视角数字全息技术

为提高数字全息再现像视角,提出一种基于滤波成像的数字全息技术来实现大视角的三维物体面型测量.利用离轴像面数字全息技术,通过在4F相干图像处理系统的空间频谱面处放置可移动的低通滤波器,使满足CCD分辨率的物光波与参考光波干涉形成全息图,并控制低通滤波及成像区域分别记录不同谱段的子全息图.再现时,首先对子全息图进行数字傅里叶变换,重构对应频谱段,并对频谱段进行拼接形成完整的物光频谱|而后通过数字再现获得大视角的数字全息再现像.利用该方法测量了圆柱形表面（光滑的缝纫针）的三维形貌,并取得了较好的实验结果.     

数字无透镜傅里叶变换全息术中非傍轴及离焦像差的校正

对数字无透镜傅里叶变换全息图直接采用逆傅里叶变换进行物场的数值重建时.需要满足两个条件:第一,全息图的记录过程必须满足傍轴近似条件,否则再现过程中会产生非傍轴像差;第二,记录全息图时物平面与参考点光源到全息图记录平面的距离必须相等,否则再现过程中会产生离焦像差.理论分析了非傍轴及离焦记录条件下数字无透镜傅里叶变换全息图的灰度分布特点,并提出了相应的非傍轴及离焦像差的数值校正方法.根据实际的非傍轴或离焦记录情况.分别给所记录的数字全息图灰度分布矩阵乘以适当的非傍轴或离焦校正因子,以消除灰度矩阵中非傍轴或离焦因素的影响.然后再对校正后的伞息图灰度矩阵做逆傅里叶变换处理.即可得到准确的数字再现像.实验结果表明.该数值重建方法能够有效地消除无透镜傅里叶变换全息术中数字再现像的非傍轴像差及离焦像差,提高再现像的质量.     

High resolution digital holographic microscopy for the study of aggregated natural cellulose nanowhisker fibers

In this paper, digital holographic (DH) microscopy demonstrates its ability to perform a full characterization of nanofibers. The high resolution and magnification of the presented method to study the nanofibers are tested using standard MIL-STD-150A 1951 USAF resolution test target. In this investigation, aggregated natural cellulose nanowhisker fibers are positioned in the front of the microscopic objective using a 3D translation stage in the object arm of DH setup. The recorded off-axis holograms are refocused using the angular spectrum method. The reconstructed complex field is used to calculate optical phase and intensity distributions of the object at different reconstruction depths. A simple algorithm is used to define the focused image with suitable accuracy. The dimensions and orientation of the fibers can be evaluated from the optical field at different depths. Then, the shape and textures along the aggregated natural cellulose nanowhisker fiber can be presented in a 3D space.     

Recovering correct phase information in multiwavelength digital holographic microscopy by compensation for chromatic aberrations

We demonstrate experimentally that correct phase imaging without 2pi ambiguity is obtainable in digital holography by using a multiwavelength approach in the microscope configuration. We describe a general approach for removing chromatic aberrations and for controlling the pixel size of the reconstructed phase image in multiwavelength digital holography when the Fourier transform method is adopted for the numerical reconstruction of digital holograms. The retrieved phase is affected by the unavoidable, unwanted chromatic aberration. The correct phase can be obtained by evaluating the phase from the reference holograms reconstructed at different wavelengths to compensate for the chromatic aberration.     

全息模拟再现像的三维重构

提出一种全息模拟再现像的三维重构方法，可以模拟再现得到三维再现像.计算机模拟再现许多幅在不同深度位置的二维光强分布；利用灰度级变化的聚焦度评价方法，通过寻找最大聚焦度值，确定再现三维像各像点的深度信息.实验证明,该方法能实现模拟再现像的三维重构，使数字全息术有希望成为一种全新的三维面形检测技术.再现像三维重构的实现可以更客观地对全息图进行像质评价，并验证计算机制全息术算法的正确性.     

像面滤波技术在无透镜傅里叶变换数字全息中的应用

无透镜傅里叶变换数字全息波前重建主要采用全息图的一次快速傅里叶变换方法,重建图像不能充分占有重建平面.本文基于像平面滤波技术,提出对物体局部区域光波场进行放大重建并让重建图像布满重建平面的方法,给出具有精细结构物体的数字全息波前重建实例.此外,将数字全息光波场重建视为具有方形出射光瞳的光学系统的相干光成像过程,导出了物体放大图像的分辨率与光学系统相关参量的关系,并通过实验给予证明.     

像面滤波技术在无透镜傅里叶变换数字全息中的应用

无透镜傅里叶变换数字全息波前重建主要采用全息图的一次快速傅里叶变换方法,重建图像不能充分占有重建平面.本文基于像平面滤波技术,提出对物体局部区域光波场进行放大重建并让重建图像布满重建平面的方法,给出具有精细结构物体的数字全息波前重建实例.此外,将数字全息光波场重建视为具有方形出射光瞳的光学系统的相干光成像过程,导出了物体放大图像的分辨率与光学系统相关参量的关系,并通过实验给予证明.