RP/HPLC测定吡虫啉血药浓度及其大鼠灌胃药代动力学评价

建立了大鼠血浆中吡虫啉的反相高效液相色谱(RP/HPLC)分析方法,并用于吡虫啉在大鼠体内的代谢动力学行为及特征研究。血浆样品经甲醇萃取,反相HPLC法测定血浆中的吡虫啉浓度。色谱柱为Agilent ZORBAX C18柱(4. 6 mm×250 mm,5μm),流动相为甲醇-水(30∶70),进样量20μL,检测波长245nm,流速1. 0 m L/min。在最佳条件下,吡虫啉的线性范围为0. 5～100 mg·L-1(r2=0. 999 8),检出限为10μg·L-1,平均加标回收率为74. 7%。大鼠单剂量灌胃给予68. 1 mg·kg-1吡虫啉,分别测定给药后不同时间下血浆中吡虫啉的浓度,并计算代谢动力学参数。灌胃给药后吡虫啉的Cmax为23. 557 mg/L,tmax为7. 333h,t1/2α为4. 472 h,AUC(0-t)为276. 727 mg·h/L。代谢动力学结果显示,吡虫啉在大鼠体内呈二隔室模型分布,以一级药代动力学方式消除。该方法简便可靠,能够满足血浆中吡虫啉测定及药代动力学研究的要求。     

酸化沉淀蛋白-超快速液相色谱-串联质谱法测定肝损伤大鼠血浆中的头孢呋辛

为了研究二代头孢类新药头孢呋辛赖氨酸在肝损伤大鼠体内的药代动力学过程,建立了采用超快速液相色谱-串联质谱(UFLC-MS/MS)快速测定肝损伤模型大鼠血浆中头孢呋辛含量的方法。血浆样品在酸性条件下用乙腈沉淀蛋白,采用Shim-pack XR-ODS色谱柱(75 mm×3.0 mm, 2.2 μm)为分析柱、乙腈-0.1%甲酸水溶液(40:60, v/v)为流动相、流速为400 μL/min进行色谱分离,采用电喷雾负离子(ESI~)模式电离、多反应监测(MRM)模式进行质谱检测,用于定量分析的离子对分别为m/z 423.2→206.8 (头孢呋辛)和m/z 454.1→238.4 (内标头孢噻肟)。结果表明,大鼠血浆中头孢呋辛的质量浓度在0.01～1 mg/L和1～400 mg/L范围内线性关系良好(r＞0.99),定量限为0.01 mg/L,日内和日间精密度(以相对标准偏差(RSD)计)均小于11.5%,准确度(RE)为~7.1%～2.2%,平均萃取回收率大于83.5%,样品运行时间仅为3.0 min,能够满足生物样品的测定需求。该法简便、快速,已用于肝损伤大鼠静脉注射头孢呋辛赖氨酸的药代动力学预实验研究。     

超高效液相色谱-串联质谱法测定大鼠血浆中的磷酸西他列汀

建立了大鼠血浆中磷酸西他列汀含量检测的超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法。以大鼠空白血浆为基质,通过添加标准品的方法配制含磷酸西他列汀和内标物氟西汀的样品,选用甲醇为沉淀剂,经离心除去血浆中的蛋白质,上清液用于目标物的检测。采用Thermo Hypersil Gold C18柱(50 mm×2.1 mm, 1.9 μm)为分析柱,Phenomenex Security Guard C18(4 mm×3.0 mm)为预柱,以乙腈和0.05%(v/v)甲酸水溶液为流动相进行梯度洗脱,流速为200 μL/min, 5 min内实现了快速分离。采用电喷雾正离子(ESI+)模式电离,选择反应监测(SRM)模式检测,确定了磷酸西他列汀和氟西汀的监测离子对分别为m/z 408.0→235.0和m/z 310.0→148.0,用基质匹配标准溶液法进行定量。结果表明: 大鼠血浆中磷酸西他列汀的质量浓度在1～1000 μg/L范围内时线性关系良好(r=0.9991),检出限(信噪比为3)为0.2 μg/L;其平均回收率为85%～115%;日内及日间的相对标准偏差(RSDs)均小于15%,满足生物样品检测的要求。将该方法初步用于大鼠静脉注射后的血浆样品中磷酸西他列汀的检测。该方法快速、灵敏度高、操作简便、重现性好,能够用于磷酸西他列汀药代动力学等方面的研究。     

新型光敏剂32-(4-甲氧基苯基)-152-天冬氨酸-二氢卟吩e6的药代动力学及组织分布特征

二氢卟吩类衍生物3²-(4-甲氧基苯基)-15²-天冬氨酸-二氢卟吩e6(DYSP-C34)是从程海湖螺旋藻中提取并合成的新型光敏剂。研究DYSP-C34在生物体内的药代动力学及组织分布过程对光动力疗法(PDT)的有效性和安全性至关重要。该文运用高效液相色谱-紫外(HPLC-UV)检测技术,建立了大鼠血浆中DYSP-C34的检测方法。采用沉淀蛋白-液液萃取法处理血浆和组织样品,采用Unitary C₁₈色谱柱(250 mm×4.6 mm, 5 μm)分离,流动相为甲醇-5 mmol/L四丁基磷酸氢铵缓冲盐溶液(70∶30, v/v),流速为1.0 mL/min,进样量为20 μL,检测波长为400 nm,柱温为40 ℃。实验结果表明,大鼠血浆药物质量浓度在1~200 μg/mL范围内线性良好,判定系数(r²)为0.9941。在低、中、高(8、40、120 μg/mL)3个添加水平下的提取回收率分别为74.39%、69.71%和65.89%,日内和日间相对标准偏差(RSD)均在5%以内。运用此方法测定静脉注射DYSP-C34(16 mg/kg)后大鼠血浆中以及荷瘤小鼠组织中的药物浓度,采用DAS 2.0计算出药物半衰期t_1/2z为6.98 h,药-时曲线下面积AUC_(0-∞)为1025.01 h·mg/L,平均驻留时间MRT_(0-∞)为9.19 h。DYSP-C34在荷瘤小鼠体内的分布结果显示,DYSP-C34可以在肿瘤组织中蓄积,并具有一定的滞留作用。综上,该文建立了大鼠血浆中DYSP-C34的HPLC-UV测定方法,并进行了方法学验证,此方法简便、快速,结果准确。阐明了DYSP-C34在静脉给药方式下大鼠体内药代动力学和荷瘤小鼠组织中的分布特征,对临床合理用药和药效学研究具有重要意义。     

柱切换技术-高效液相色谱法快速检测大鼠血浆中的普萘洛尔对映体

建立了快速检测大鼠血浆中普萘洛尔对映体浓度的柱切换-高效液相色谱法。将自制限进填料柱作为预处理柱,通过直接进样方式,使普萘洛尔对映体在预处理柱上保留,同时除去血浆中的蛋白质等大分子;再通过柱切换技术,使普萘洛尔对映体在键合型纤维素-三(3,5-二甲基苯基氨基甲酸酯)(Chiralcel OD-RH)分析柱上得到手性拆分。通过条件优化,确定切换前预处理流动相为硼酸盐缓冲液(pH 8.5)-甲醇(95:5, v/v),流速为1.0 mL/min;切换后分析流动相为异丙醇-乙醇-0.2 mmol/L硼酸盐缓冲液(pH 8.5)(30:30:40, v/v/v),流速为0.8 mL/min;切换时间为3 min;柱温为25 ℃;检测波长为293 nm。普萘洛尔两对映体在25～500 mg/L的质量浓度范围内具有良好的线性关系(r=0.9995), 3个加标水平(50、100、250 mg/L)的平均回收率为97.89%～101.56%,日内和日间精密度均小于5%。该方法简便、快速、灵敏、准确,适于血浆样本中手性药物的药代动力学研究。     

高效液相色谱-质谱法测定比格犬血浆中的艾普拉唑及其药代动力学

运用高效液相色谱-电喷雾质谱(HPLC-ESI-MS)技术,建立了快速、简单、灵敏的比格犬静脉滴注艾普拉唑钠盐后血药浓度的检测方法。血浆样品采用蛋白沉淀法,以丁螺环酮作为内标,色谱柱为Teknokroma Kromasil C18(100 mm×2.1 mm, 5 μm),流动相为水-甲醇-乙腈(69:8:23, v/v/v)(含0.1%的甲酸),流速0.2 mL/min,采用电喷雾(ESI)离子源以正离子方式检测。绘制血药浓度-时间曲线,并采用DAS 2.0计算药代动力学参数。方法学实验结果表明内源性杂质不干扰艾普拉唑和内标的测定,线性范围为5～10000 μg/L (r=0.994),最低定量限为5 μg/L,精密度和准确度均符合生物样品测定的要求。低、中、高3个浓度的绝对回收率在106%左右,基质效应小于142.0%,表明该方法适合比格犬血浆中艾普拉唑浓度的测定及药代动力学研究。比格犬静脉滴注艾普拉唑钠盐3个剂量(0.2 mg/kg、0.8 mg/kg和3.2 mg/kg)后的药-时曲线下面积(AUC(0~∞))分别为(2.4×104±3×103)、(8.8×104±1.6×104)和(5.4×105±8×104) μg/L•min,呈线性药物代谢动力学过程。     

鳗鲡血浆中盐酸环丙沙星的反相高效液相色谱法测定

利用反相高效液相色谱(RP-HPLC)建立鳗鲡血浆中盐酸环丙沙星(CPFX)残留的测定方法.采用RP ODS色谱柱,以甲醇-四丁基溴化铵(30:70 v/v)为流动相,流速1 mL/min,进样量20μL,荧光激发波长(Ex)277 nm,荧光检测波长(Em)453 nm.鳗鲡血浆样品用二氯甲烷进行提取前处理,然后进行HPLC分离-荧光检测.盐酸环丙沙星溶液浓度在0.01～10.24 μg/mL范围与色谱峰高呈良好线性关系(r=0.9997),最低检测限为0.005μg/mL,标准添加平均回收率大于80%.方法灵敏简便,可用于鳗鲡体内盐酸环丙沙星残留的测定与药代动力学研究.     

LC-MS/MS法测定大鼠血浆中人参皂苷Rb1的含量及其药代动力学研究

采用液相色谱-串联质谱法快速、灵敏地测定大鼠血浆中人参皂苷Rb1(GRb1)的含量,并将该方法应用于大鼠口服GRb1后的代谢动力学研究。血浆样品采用96孔板进行液-液萃取后,应用Agilent SB-C18色谱柱(100 mm×2.1 mm,3.5μm)进行分离,以甲醇-0.1%甲酸溶液(体积比为75∶25)为流动相进行洗脱,在正离子模式下对GRb1和内标人参皂苷Rg1(GRg1)进行检测,用于定量的离子反应分别为1131.5→365.1(GRb1),823.3→643.4(GRg1)。人参皂苷Rb1血浆样品测定方法的定量线性范围为1~500 ng/mL,线性相关系数大于0.999,定量下限为1 ng/mL,批内和批间精密度(RSD)小于9.05%,回收率为79.7%~81.0%,基质效应为96.6%~99.3%。大鼠灌胃给予Rb15 mg/kg后,大鼠体内血药浓度到达高峰时间tmax为1.53 h,半衰期t1/2为13.54 h,药时曲线面积AUC0~72为16237.76(ng·h)/mL。该方法快速、高效、灵敏,适用于人参皂苷Rb1的代谢动力学研究。     

人参皂苷Re在正常和中波紫外线辐射损伤模型大鼠体内药代动力学比较研究

建立了超高效液相色谱-三重四级杆质谱(UPLC-QQQ-MS)检测大鼠血浆中G-Re含量的方法,用于比较研究人参皂苷Re(Ginsenoside Re,G-Re)在正常大鼠和UVB辐射损伤模型大鼠体内的药代动力学行为。选用Ascentis~? Express C_(18)色谱柱(5.0 mm×3.0 mm,2.7μm),以0.1%甲酸-乙腈为流动相,梯度洗脱。电喷雾离子源(ESI)在负离子模式下,选择多反应监测模式(MRM)扫描,内标法定量,用于G-Re定量分析的离子为m/z 991.54/945.53/475.60。方法检出限为4.0 ng/m L,定量限为13.5 ng/m L,G-Re在15~20000 ng/m L范围内线性关系良好(r=0.999);日内和日间精密度、回收率、基质效应和稳定性均能满足药代动力学分析要求。结果表明,两组大鼠分别单次口服50 mg/kg G-Re后,体内代谢过程表现皆符合二室模型特征,正常组和模型组t_(1/2α)分别为(0.21±0.04)h和(0.69±0.07)h,t_(1/2β)分别为(17.08±0.53)h和(21.40±16.77)h,AUC_((0-t))分别为(321.91±2.27)"g/(L·h)和(474.99±194.96)"g/(L·h),AUC_((0-∞))分别为(332.44±1.66)"g/(L·h)和(518.64±231.39)"g/(L·h),除t_(1/2α)外,两组之间的药代动力学参数具有显著差异(p0.05)。本方法准确、灵敏、专属性强、稳定性好,可用于人参皂苷Re的体内药代动力学比较研究。     

超高效液相色谱-串联三重四极杆质谱分析法对大鼠血浆中三氯生的代谢和动力学研究

三氯生是一种被广泛应用在家庭卫生用品中的抗菌消毒剂。虽然其本身不具有很强的毒性,但其在生物体内的代谢变化是否对生物和人体有害我们还不得而知。因此,研究三氯生在动物体内的代谢与动力学情况是具有重要意义的。本文采用超高效液相色谱串联三级四极杆质谱法来测定口服给药(5 mg/kg)后大鼠血浆中的三氯生的含量及其代谢产物。相对于多反应监测(MRM)技术,尽管其有较好的最低检测限,但选择离子监测(SIR, 又称为SIM)有更好的方法验证参数。在本试验中,选择离子监测方法检测限为10.8 ng/mL,方法的回收率、准确度、精密度和重现性都较高。用该方法测定的三氯生在大鼠体内的消除半衰期为(48.5±10.5) h。同时,还鉴定出其三氯生血浆中有分别被羟基化加磺酸化、葡萄糖醛酸化以及磺酸化的4个代谢产物。     

Simultaneous Determination of Caffeine and Theophylline in Human Plasma with a Weak Cation Monolithic SPE-column

A simple reversed‐phase high performance liquid chromatography (RP‐HPLC) method was developed to determine the level of caffeine and theophylline in human plasma samples. The sample clean‐up step involved the on‐line solid‐phase extraction (SPE) of the analytes from plasma samples into a weak cation monolithic column using a column switching system. Separation was performed on a C₁₈ column (5 µm, 150 mm×4.6 mm) with ultraviolet detection at 274 nm. The mobile phase consisted of methanol‐water (32/68, V/V) under isocratic conditions at a flow rate of 0.6 mL·min⁻¹. The measured concentration of caffeine and theophylline showed a good linear relationship over the concentrations range, 0.1–80.0 µg·mL⁻¹. The absolute recoveries ranged from 77.10% to 85.39%, and the inter‐day and intra‐day relative standard deviations (RSD) were all less than 5%. This method avoids a tedious pretreatment and provides an economic, repeatable and effective method for assaying trace drugs in biological samples.     

High-performance Liquid Chromatographic Analysis of Pioglitazone,Gliquidone,Rosuvastatin and Simvastatin in Formulations and Human Serum

Co‐administration of HMG‐CoA reductase with antidiabetic drugs is most common since antidiabetic drugs are mostly prescribed for long term therapy. In the present paper, we describe the simultaneous determination of antidiabetic (pioglitazone hydrochloride and gliquidone) in presence of statins (rosvastatin and simvastatin) in formulations and in human serum using RP‐HPLC technique. The serum samples were subjected to protein precipitation with acetonitrile prior to an HPLC analysis. At a flow rate of 1 mL·min⁻¹ isocratic elution was employed, using mobile phase consisting of methanol/water (90:10, V:V), pH 3.50 with phosphoric acid and absorbance was recorded at 235 nm. The assay was reproducible, linear (concentration range of 5–50 μg·mL⁻¹) and accurate. The LOD and LOQ values were 1.32, 0.28, 0.05 and 0.57 μg·mL⁻¹ and 4.39, 0.93, 0.16 and 1.90 μg·mL⁻¹ for pioglitazone hydrochloride, gliquidone, rosvastatin and simvastatin, respectively. There were no interfering peaks due to the excipients present in the pharmaceutical tablet and serum. Thus, the proposed method is simple and suitable for the analysis of active ingredient in tablet form and human serum.     

Solid‐phase Extraction of β‐Sitosterol from Oldenlandia diffusa Using Molecular Imprinting Polymer

The β‐sitosterol imprinted polymer was prepared for selective extraction and analysis of β‐sitosterol from Oldenlandia diffusa (O. diffusa) followed by HPLC with UV detection. The imprinted polymers show high affinity and selectivity to β‐sitosterol. Using this molecularly imprinted polymer (MIP) cartridge as solid‐phase extraction (SPE) material, the interferences could be quickly washed out and β‐sitosterol was selectively retained and enriched. HPLC analysis method was used to evaluate the characteristics of this MIP material. At the condition of mobile phase composed of MeOH/H₂O/H₃PO₄ (99/1/0.1, V/V/V, pH=6.0) and the flow rate of 1.0 mL·min^?1, a good linear relationship was demonstrated when the concentrations of β‐sitosterol were in the range of 0.50–100.0 µg·mL^?1. The recoveries ranged from 75.3% to 86.5% and the inter‐day and intra‐day relative standard deviations were less than 5%. This developed HPLC method was proved to be acceptable for extraction of β‐sitosterol from O. diffusa.     

Preparation,antitumor activity in mice,pharmacokinetics and tissue distribution in rats of di‐n‐butyl‐di‐(4‐chlorobenzohydroxamato)tin(IV) liposome

Di‐n‐butyl‐di‐(4‐chlorobenzohydroxamato)tin(IV) (DBDCT) is an antitumor compound with high activity and relatively low bioavailability. In order to improve the pharmacokinetic characteristics and raise its therapeutic index, a liposome of DBDCT (DBDCT‐L) was prepared for the first time. A study of the pharmacokinetics and tissue distribution after intravenous administration of DBDCT‐L compared with free DBDCT to rats was investigated. DBDCT‐L showed a slower clearance, increased half‐time and a larger AUC value than those of free DBDCT, which demonstrated that DBDCT‐L could significantly alter the tissue distribution pattern of DBDCT in rats. The highest concentration distribution for DBDCT‐L was detected in liver, which may be associated with the enhanced antitumor activity in vivo against hepatocellular carcinoma H₂₂ and possible target release of the compound. Acute toxicity assay showed that the LD₅₀ value of DBDCT‐L was higher than that of free DBDCT. Further in vivo antitumor test showed that DBDCT‐L displayed higher antitumor activity against the hepatocellular carcinoma H₂₂ than free DBDCT, indicating that the liposome could prolong the action time of DBDCT in the system circulation, change its distribution in rats, reduce acute toxicity and finally increase antitumor activity. Copyright © 2014 John Wiley & Sons, Ltd.     

№Determination of Trace Arsenic by Solid Substrate-Room Temperature Phosphorescence Quenching Method Based on the Catalyzed Reaction of H2O2 Oxidizing 9-Hyd roxy-2,3,4,9-tet rahyd ro-1,10-anth raquinone

A new solid substrate-room temperature phosphorescence （SS-RTP） quenching method for the determination of trace As（V） has been developed, based on the facts that 9-hydroxy-2,3,4,9-tetrahydro-1,10-anthraquinone （R） can emit intense and stable SS-RTP on solid substrate, and α,α＇-dipyridyl can activate As（V） catalysis of the reaction of H2O2 oxidizing R to non-phosphorescence compound R＇, which can cause the sharp quenching of SS-RTP. Under the optimum condition, the relationship between the ΔIp of the emitting intensity and 1.60-160 fg·spot^-1 As（V） （corresponding concentration： 0.0040-0.40 ng·mL^-1, sample volume： 0.4 μL·spot^-1） conformed to Beer＇ law. The regression equation of working curve can be expressed as ΔIp= 20.46＋0.5492CAs（v） fig·spot^-1） （r= 0.9995, n = 6）. The limit detection （LD） is 0.27 fg·spot^-1 [As（V） corresponding concentration： 6.8 × 10^-13 g·mL^-1, n=11]. The samples containing 0.0040 and 0.40 ng·mL^-1 As（V） were repeatedly determined for 11 times. RSD are 3.0% and 2.7% respectively. The SS-RTP mechanism was also discussed. R was synthesized in this paper. Meanwhile, the structure was determined by NMR, IR, mass spectra and elemental analysis.     

Design,Synthesis and Biological Evaluation of Novel N‐(4‐([2,2′:5′,2′′‐Terthiophen]‐5‐yl)‐2‐methylbut‐3‐yn‐2‐yl) Benzamide Derivatives

On the basis of the principle of combination of active groups, a series of novel N‐(4‐([2,2′:5′,2′′‐terthiophen]‐5‐yl)‐2‐methylbut‐3‐yn‐2‐yl) benzamide derivatives were designed, synthesized and systematically evaluated for their antiviral activity against tobacco mosaic virus (TMV). The bioassay results showed that most of these compounds displayed good anti‐TMV activity, and some of them exhibited higher antiviral activity than commercial Ningnanmycin. Especially, compound 8e with excellent anti‐TMV activity (inactivation activity, 92.3%/500 µg·mL^?1; curative activity, 85.7%/500 µg·mL^?1 and protection activity, 64.7%/500 µg·mL^?1) emerged as a potential inhibitor of plant virus TMV. Quantitative structure‐activity relationship studies proved that the van der Waals volume (V) and electronic parameter (∑(∑σ_o+σ_p) and ∑σ_m) for the substituent R¹ were very important for antiviral activities in this class of compounds.     

Voltammetric Determination of Rosiglitazone in Pharmaceutical Preparations and Human Plasma

Abstract

The voltammetric behavior of rosiglitazone was studied using direct current (DC_t), differential pulse (DPP), and alternating current (AC_t) polarography. The drug manifests cathodic waves over a pH range of 2–11.2. In Britton‐Robinson buffer (BRb; pH 4), the diffusion current–concentration relationship was found to be rectilinear over a range of 4–24 µg · mL^?1 and 0.1–16 µg · mL^?1 using DC_t and DPP modes, respectively, with minimum limits of detection (LOD) of 0.15 µg · mL^?1 and 0.07 µg · mL^?1 using the DC_t and DDP modes, respectively. The diffusion‐current constant (I _d) was 6.63±0.03 (n=5). The proposed method was successfully applied to the determination of the studied compound both in pure form and in formulations. The mean percentage recoveries in tablets were 100.09±1.18 and 100.85±0.88 (n=5) using DC_t and DPP modes, respectively. Furthermore, the proposed method, adopting the DPP mode, was applied to the determination of rosiglitazone in spiked human plasma and the obtained mean percentage recoveries were 99.14±3.29 (n=4).     

Development and validation of a generic RP‐HPLC PDA method for the simultaneous separation and quantification of active ingredients in cold and cough medicines

A novel generic reverse phase high performance liquid chromatography (RP‐HPLC) method is developed and validated for simultaneous determination of seven pharmaceutically active ingredients, namely, acetaminophen, dextromethorphan, doxylamine, phenylephrine, guaifenesin, caffeine and aspirin. All seven ingredients were quantified in soft gel, syrup and tablet formulations of the over‐the‐counter US‐marketed products, as per the guidelines of the International Conference on Harmonization. The separation was achieved in a 16 min run time on an Agilent Zorbax Phenyl column using a gradient method with two mobile phases. Mobile phase A was 0.15% trifluoro acetic acid in purified water and while mobile phase B was a mixture of acetonitrile and methanol (750:250 v/v) with 0.02% trifluoro acetic acid. The flow rate was 1.0 mL min^?1 and injection volume was 10 μL. Detection was performed at 280 nm using a photodiode array detector. As part of the method validation, specificity, linearity, precision and recovery parameters were verified. The concentration and area relationships were linear (R² > 0.999), over the concentration ranges 20–120 μg mL^?1 for acetaminophen, 75–450 μg mL^?1 for dextromethorphan, 31.25–187.5 μg mL^?1 for doxylamine, 25–150 μg mL^?1 for phenylephrine, 25–150 μg mL^?1 for aspirin, 6.5–39 μg mL^?1 for caffeine and 12–72 μg mL^?1 for guaifenesin. The relative standard deviations for precision and intermediate precision were <1.5%. The proposed RP‐HPLC generic method is applicable for routine analysis of cold and cough over‐the‐counter products.     

Study on the Interaction between a Novel Ionic Liquid Probe and Anionic Surfactants Using Resonance Light Scattering Spectra and Its Analytical Application

The interaction between anionic surfactants (AS) and 1‐hexadecyl‐3‐methylimidazolium bromide [C₁₆mim]Br was studied by using resonance light scattering (RLS) technique, UV‐Vis spectrophotometry and fluorometric methods. In Britton Robinson (BR) buffer (pH 6.0), [C₁₆mim]Br reacted with AS to form supermolecular complex which resulted in enhancement in RLS intensity. Their maximum RLS wavelengths were all at 390 nm. Some important interacting experimental variables, such as the solution acidity, [C₁₆mim]Br concentration, salt effect and addition order of the reagents, were investigated and optimized. Under the optimum conditions, quantitative determination ranges were 0.001–7 μg·mL^?1 for dodecyl sodium sulfate (SDS), 0.001–6 μg·mL^?1 for sodium dodecylbenzene sulfonate (SDBS) and 0.005–7 μg·mL^?1 for sodium lauryl sulfonate (SLS), respectively, while the detection limits were 1.3 ng·mL^?1 for SDS, 1.0 ng·mL^?1 for SDBS and 5.1 ng·mL^?1 for SLS, respectively. Based on the ion‐association reaction, a highly sensitive, simple and rapid method has been established for the determination of AS.