基于血红蛋白/纳米金修饰离子液体电极的电化学生物传感器

氧化还原蛋白质在工作电极上的直接电化学对于研究生命体系的电子转移机理,了解生命过程中的氧化还原机理,开发新型电化学生物传感器有着重要的意义~([1]).目前较多的工作是利用各种媒介体、促进剂和纳米材料修饰电极来实现蛋白质的直接电子转移.离子液体修饰电极(CILE)是以离子液体为修饰剂和粘合剂的一种新型化学修饰电极,在生物电分析化学已经应用.本文在CILE表面修饰纳米金用于血红蛋白的固定及其直接电化学行为的研究,取得了较好的结果.     

蛋白质直接电化学研究及其应用

蛋白质直接电化学研究在生物电化学中具有重要地位,对于蛋白质结构-功能研究、蛋白质电子传递过程的热力学和动力学研究都有着重要意义,而且是研制第三代电化学生物传感器的基础。本文对在裸电极、分子自组装修饰电极和模拟生物膜修饰电极上进行蛋白质直接电化学的研究及相关应用进行简要综述。     

蛋白质直接电化学研究及其应用

蛋白质直接电化学研究在生物电化学中具有重要地位,对于蛋白质结构-功能研究、蛋白质电子传递过程的热力学和动力学研究都有着重要意义,而且是研制第三代电化学生物传感器的基础.本文对在裸电极、分子自组装修饰电极和模拟生物膜修饰电极上进行蛋白质直接电化学的研究及相关应用进行简要综述.     

蛋白质直接电化学研究及其应用

蛋白质直接电化学研究在生物电化学中具有重要地位，对于蛋白质结构．功能研究、蛋白质电子传递过程的热力学和动力学研究都有着重要意义，而且是研制第三代电化学生物传感器的基础。本文对在裸电极、分子自组装修饰电极和模拟生物膜修饰电极上进行蛋白质直接电化学的研究及相关应用进行简要综述。     

电化学检测磷酸化多肽的研究

建立了一种非探针标记型磷酸化多肽的电化学检测方法,该方法以N,N-二(羧甲基)-L-赖氨酸(Lys-NTA)和Fe3+修饰的金电极作为磷酸化多肽的捕获和传感界面,利用磷酸化多肽能够与之形成金属离子螯合物而发生特异性结合并引起电极表面修饰层通透性改变等特点,采用铁氰化钾作为电化学指示剂,用循环伏安法和交流阻抗法对磷酸化多...     

蛋白质组学中磷酸化肽的常用富集方法

磷酸化作用是最重要的蛋白质翻译后修饰方式之一,它是蛋白质组学的一个重要分支,在细胞识别、细胞信息传递、基因表达和新陈代谢等方面发挥着重要作用。采用适当方法对磷酸化肽进行分析有助于我们更好地了解生理病理机制。但是直接进行质谱分析时磷酸化肽的信号强度会受到无机盐以及大量非磷酸化肽的抑制,选择性差。为解决这一难题,在质谱分析前要对磷酸化肽进行选择性富集。本文回顾了几种常用的磷酸化肽富集方法,介绍了每种方法的发展状况和常用材料,其中包括固定金属离子亲和色谱法、金属氧化物富集法、强阴阳离子交换色谱法和MALDI靶板富集法。最后总结了各种富集方法的优缺点,对有效的磷酸化肽富集策略进行了前景展望。     

磷元素参与的生命活动

磷元素在人体内的首次发现暗藏了神秘色彩,也闪耀了生命之光。简要介绍了与生命活动息息相关的磷元素,遗传物质是由磷酸骨架组成的,细胞膜是由磷脂双分子层构成的,蛋白质的磷酸化修饰能够调节生命活动并与许多疾病的发生有关。     

蛋白质的电分析化学研究

生命现象的许多过程皆伴随着电子传递反应，应用电化学方法研究生物体系的电子传递及其相关过程是揭示生命本质的较好途径。本文从酶生物传感器、蛋白质修饰电极和免疫传感器3个方面评述了近年来蛋白质的电分析化学研究的现状和进展，并提出了今后可能的研究方向。     

磷钼钒类杂多酸—聚吡咯薄膜修饰电极及其电催化性能研究

合成了磷钼类杂多酸，采用电化学方法首次在导电基体玻碳电极上研制了磷钼钒类杂多酸－聚吡咯薄膜修饰电极，该电极性能稳定，经久耐用，对膜修饰电极的电化学行为进行了表征，研究了膜修饰电极酸性水溶液中的氯酸根、溴酸根，、磺酸根，亚硝酸根，三阶铁离子，     

基于多肽的电化学传感器在蛋白质检测中应用的研究进展

生物体内的细胞通常会分泌各种各样的蛋白质,这些蛋白质在生物体中发挥着重要作用,尤其是可被用于诊断各种疾病的发生和发展。多肽具有良好选择性、空间适应能力和识别灵活的特点,可与不同类型的蛋白分子形成非共价键,用于蛋白质的生物检测。将多肽与电化学生物传感器结合用于蛋白质的广谱检测具有良好的发展前景。本文介绍了多肽修饰的电化学传感器在不同蛋白质检测方面的研究进展,分析了待测蛋白质的不同对多肽修饰的电化学传感器分类的影响及其优缺点,提出了基于多肽的电化学传感器在不同蛋白质检测中存在的问题,并展望了其未来发展。     

二氧化碳和丙烯直接合成甲基丙烯酸的NiPMo/TiO2催化剂的研究

用溶胶-凝胶法以磷钼酸(MPA)的镍盐溶液水解钛酸四丁酯制备了NiPMo/TiO2催化剂.使用ICP、 XRD、 TG-DTA、 IR、 TPD-MS和微反应技术研究了催化剂的化学组成、热稳定性、化学吸附性质和催化反应性能.杂多钼酸盐与TiO2通过O2-在TiO2表面发生了键合.在623 K下,杂多阴离子仍保持原有的Keggin结构.CO2在Lewis酸位Ni(Ⅱ)和Lewis碱位Ni-O-Mo的桥氧协同作用下生成CO2卧式吸附态Ni(Ⅱ)←O-(CO)←(O--Ni).丙烯有多种吸附态在催化剂上吸附.在563 K、 1 MPa和空速1500 h-1的反应条件下,丙烯的摩尔转化率为3.2%,产物MAA选择性为95%.     

Toward new camptothecins. Part 6: Synthesis of crucial ketones and their use in Friedländer reaction

In the context of the preparation of camptothecin and luotonin A analogs, the synthesis of some key keto-precursors and their use in Friedländer condensation are described. This paper also focuses on the stability of these keto intermediates and emphasizes the major differences between indolizinones and pyrroloquinazolinones series. Noteworthy is also the report of some original structures isolated as by-products of some experiments.     

α-Amino acid derived enaminones and their application in the synthesis of N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates and other heterocycles

A new and simple synthesis of novel N-protected methyl 5-substituted-4-hydroxypyrrole-3-carboxylates, which exist in equilibrium with their 4-oxo tautomers, has been developed in two steps starting from N-protected α-amino acids. The key intermediates are enaminones, which can also be isolated, characterized, and used for the construction of other functionalized heterocycles, before they spontaneously decompose to pyrrole products. 4-Hydroxypyrroles are prone to partial aerial oxidation but can be efficiently alkylated or reduced to stable polysubstituted pyrrolidine derivatives.     

Specific intramolecular aromatic CH insertion of diazosulfonamides

The chemoselectivity in the intramolecular CH insertion of various diazosulfonamides has been experimentally studied. The results reveal that the aliphatic 1,4-, 1,5-, or 1,6-C(sp³)?H insertions of diazosulfonamides are not accessible, while the aromatic 1,5-C(sp²)?H insertion can be realized specifically by adjusting the diazo-adjacent group. In addition, the general chemoselectivities in the intramolecular CH insertions of diazosulfonyl compounds are summarized. Generally, diazosulfones undergo both aromatic 1,5-C(sp²)?H and aliphatic 1,5- and 1,6-C(sp³)?H insertions, while diazosulfonates undergo aliphatic 1,5- and 1,6-C(sp³)?H insertions. However, diazosulfonamides only undergo aromatic 1,5-C(sp²)?H insertion.     

CoFe2O4自形成磁性液体场致结构化对磁化的影响

The Langevin paramagnetic theory can’t describe the relation between magnetization of ferrofluids and applied magnetic field. The structuralization of ferrofluids, which is considered the main influence factor of the magnetization, is regarded. The part of magnetization works is deposited when the structure is forming. This action influences the magnetization of ferrofluids directly or indirectly. On the base of the “compressing” model, the Langevin function that usually describes the magnetization of ferrofluid is modified, and a well-fitted curve is obtained. An equation of the relation between the equivalent volume fraction after being “compressed” and the intensity of magnetic field is discovered, which approximately describes the process of magnetization. The relation between the approximate initial susceptibility and the volume fraction can be obtained from modified formula.     

KMnO4-mediated oxidative CN bond cleavage of tertiary amines: Synthesis of amides and sulfonamides

KMnO₄-mediated oxidative CN bond cleavage of tertiary amines producing secondary amine was introduced, which was trapped by electrophiles (acyl chloride and sulfonyl chloride) to form amides and sulfonamides. The reaction could take place at mild condition, tolerating a wide range of function groups and affording products in moderate to excellent yields.     

Highly regioselective Buchwald–Hartwig amination at C-2 of 2,4-dichloropyridine enabling a novel approach to 2,4-bisanilinopyridine (BAPyd) libraries

The highly regioselective Buchwald–Hartwig amination at C-2 of the cheap and readily accessible reagent, 2,4-dichloropyridine with a range of anilines and heterocyclic amines is described. This new methodology is robust and provides a facile access to 4-chloro-N-phenylpyridin-2-amines on 0.25 mol scale. These intermediates undergo a further Buchwald–Hartwig amination at higher temperature to enable rapid exploration of the chemical space at C-4 and to provide a library of 2,4-bisaminopyridines.     

Chemistry of heterocyclic compounds at the A. E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch of the Russian Academy of Sciences, over 50 years (Review)

The review contains a concise historical account and information on the most significant researches undertaken by the staff at the A. E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch of the Russian Academy of Sciences on the Chemistry of Heterocyclic Compounds. Dedicated to Academician of the Russian Academy of Sciences B. A. Trofimov on his 70th jubilee. Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 10, pp. 1443–1502, October, 2008.     

N-Heterocyclic carbene-palladacyclic complexes: synthesis,characterization and their applications in the C-N coupling and α-arylation of ketones using aryl chlorides

N-Heterocyclic carbene-palladacyclic complexes 3 were successfully achieved in a one-pot procedure under mild conditions. The structure of 3a was unambiguously confirmed by X-ray single crystal diffraction and it was an active catalyst in the Buchwald-Hartwig amination and α-arylation of ketones even at very low catalyst loadings (0.01?mol%).     

Iodine-mediated oxidative Pictet-Spengler reaction using terminal alkyne as the 2-oxoaldehyde surrogate for the synthesis of 1-aroyl-β-carbolines and fused-nitrogen heterocycles

An efficient iodine-mediated oxidative Pictet-Spengler reaction in dimethyl sulphoxide (DMSO) using terminal alkynes as the 2-oxoaldehyde surrogate for the synthesis of aryl (9H-pyrido[3,4-b]indol-1-yl)methanones is described. The scope of the protocol includes the total synthesis of Fascaplysin, Eudistomins Y₁ and Y₂. The methodology is extended for preparing pyrrolo[1,2-a]-quinoxaline and indolo[1,5-a]quinoxaline derivatives. The utility of 1-aroyl-β-carbolines was demonstrated by performing palladium-catalyzed β-carboline directed ortho-C(sp2)-H functionalization of the phenyl ring with thiomethyl (SMe) group using DMSO as source and for accessing 4-aryl-canthin-6-ones.