Estimation of speed of sound in dual-layered media using medical ultrasound image deconvolution

The speed of sound in soft tissues is usually assumed to be 1540 m/s in medical pulse-echo ultrasound imaging systems. When the true speed is different, the mismatch can lead to distortions in the acquired images, and so reduce their clinical value. Previously we reported a new method of sound-speed estimation in the context of image deconvolution. Unlike most other sound-speed estimation methods, this enables the use of unmodified ultrasound machines and a normal scanning pattern. Our approach was validated for largely homogeneous media with single sound speeds. In this article, we demonstrate that sound speeds of dual-layered media can also be estimated through image deconvolution. An ultrasound simulator has been developed for layered media assuming that, for moderate speed differences, the reflection at the interface may be neglected. We have applied our dual-layer algorithm to simulations and in vitro phantoms. The speed of the top layer is estimated by our aforesaid method for homogeneous media. Then, when the layer boundary position is known, a series of deconvolutions are carried out with dual-layered point-spread functions having different lower-layer speeds. The best restoration is selected using a correlation metric. The error level (e.g., a mean error of −9 m/s with a standard deviation of 16 m/s) for in vitro phantoms is found to be not as good as that of our single-speed algorithm, but is comparable to other local speed estimation methods where the data acquisition may not be as simple as in our proposed method.     

Instant magnetic labeling of tumor cells by ultrasound in vitro

Magnetic labeling of living cells creates opportunities for numerous biomedical applications. Here we describe an instantly cell magnetic labeling method based on ultrasound. We present a detailed study on the ultrasound performance of a simple and efficient labeling protocol for H-22 cells in vitro. High frequency focus ultrasound was investigated as an alternative method to achieve instant cell labeling with the magnetic particles without the need for adjunct agents or initiating cell cultures. Mean diameter of 168 nm dextran-T40 coated superparamagnetic iron oxide (SPIO) nanoparticles were prepared by means of classical coprecipitation in solution in our laboratory. H-22 tumor cells suspended in phosphate-buffered saline (PBS, pH=7.2) were exposed to ultrasound at 1.37 MHz for up to 120 s in the presence of SPIOs. The cellular uptake of iron oxide nanoparticles was detected by prussion blue staining. The viability of cells was determined by a trypan blue exclusion test. At 2 W power and 60 s ultrasound exposure in presence of 410 μg/ml SPIOs, H-22 cell labeling efficiency reached 69.4±6.3% and the labeled cells exhibited an iron content of 10.38±2.43 pg per cell. Furthermore, 95.2±3.2% cells remained viable. The results indicated that the ultrasound protocol could be potentially applied to label cells with large-sized magnetic particles. We also calculated the shear stress at the 2 W power and 1.37 MHz used in experiments. The results showed that the shear stress threshold for ultrasonically induced H-22 cell reparable sonoporation was 697 Pa. These findings provide a quantitative guidance in designing ultrasound protocols for cell labeling.     

Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology

The present study optimized the ultrasound assisted extraction (UAE) conditions to maximize the antioxidant activity [Ferric ion Reducing Antioxidant Power (FRAP)], total phenol content (TP) and content of individual polyphenols of extracts from marjoram. Optimal conditions with regard to amplitude of sonication (24.4-61.0 μm) and extraction temperature (15-35 °C) and extraction time (5-15 min) were identified using response surface methodology (RSM). The results showed that the combined treatment conditions of 61 μm, 35 °C and 15 min were optimal for maximizing TP, FRAP, rosmarinic acid, luteolin-7-O-glucoside, apigenin-7-O-glucoside, caffeic acid, carnosic acid and carnosol values of the extracts. The predicted values from the developed quadratic polynomial equation were in close agreement with the actual experimental values with low average mean deviations (E%) ranging from 0.45% to 1.55%. The extraction yields of the optimal UAE were significantly (p < 0.05) higher than solid/liquid extracts. Predicted models were highly significant (p < 0.05) for all the parameters studied with high regression coefficients (R²) ranging from 0.58 to 0.989.     

In vitro effects of ultrasound with different energies on the conduction properties of neural tissue

The effect of ultrasound at various energy levels on the conduction properties of neural tissue is explored in this in vitro study. Excised sciatic nerves from the bullfrog were used for experiments. The nerves were stimulated by 3.5 MHz continuous wave ultrasound at 1, 2, and 3 W for 5 min. The peak-to-peak amplitude of the electrically evoked compound action potential (CAP) and the conduction velocity (CV) were measured in the nerves before and during ultrasound stimulation. The CV of the nerves increased by 5-20% for ultrasound stimulations at 1-3 W. The CAP amplitude increased by 8% during stimulation with 1 W ultrasound, and progressively decreased for 2 and 3 W ultrasound. This indicates that the effect of lower energy ultrasound increases both the CV and the CAP amplitude and that the reduction in the CAP amplitude for higher energy ultrasound is associated largely with ultrasonic thermal effects.     

In vivo non-mechanical scanning grating-generated optical coherence tomography using an InGaAs digital camera

We demonstrated in vivo cross-sectional imaging of human fingers by non-mechanical scanning optical coherence tomography (OCT), using a diffracted light as the reference beam and a linear illumination beam at a center wavelength of 1.3 μm for deeper penetration into biological tissues. By applying the three-step phase-shifting method, our system can measure OCT images at 10 frames/s with a sensitivity of 90 dB for a 2.45 × 4.80 mm (axial × lateral) measurement range using an InGaAs digital camera (320 × 256 pixels).     

Novel device to measure critical point "Onset" of capillary wave and interpretation of Faraday instability wave by numerical analysis

A capillary wave was created on a surface by vibrating from the bottom of a container. When the amplitude of the container vibration approached the critical point, called the onset state, the surface broke up and bursted into very small drops on the air. The numerical analysis was used to determine the amplitude of the onset. The onset point was found to be 0.349 μm at f = 500 kHz. The critical amplitude h_cr was determined by using a multi-Fourier horn nozzle (MFHN) device. The onset point was measured to be 0.37 μm using a laser Doppler vibrometer (LDV) with the MFHN at f = 486 kHz. These drops indicate that particle size distributions of 10.8 μm and 7.0 μm were produced by the MFHN at f = 289 kHz and f = 486 kHz, respectively. These results agreed with those obtained using Kelvin’s equation, which predicted D = 0.34λ.     

Photocarrier transport in iron-doped potassium lithium tantalate niobate studied by time-of-flight measurement

The photocarrier mobility of Fe 0.03 wt%-doped potassium lithium tantalate niobate (K_0.95Li_0.05Ta_0.61Nb_0.39O₃) was investigated by time-of-flight (TOF) measurement. The longitudinal photocarrier response due to pulsed excitation leads to values of the drift mobility of μ_h = 1.45 × 10⁻² cm²/V s for holes, μ_e = 0.325 × 10⁻² cm²/V s for electrons, and a value for the range of holes (μτ)_h = 4.38 × 10⁻⁵ cm²/V at room temperature and at low field 3 KV/cm. The response time of holes and electrons (or the relaxation time) is determined to be 3.02 × 10⁻³ s and 3.74 × 10⁻³ s, respectively. The mobility of holes strongly depends on the field strength, and is observed to decrease with increasing bias field.     

Strategies for reliable automatic onset time picking of acoustic emissions and of ultrasound signals in concrete

Determining the onset of transient signals like seismograms, acoustic emissions or ultrasound signals is very time consuming if the onset is picked manually. Therefore, different approaches exist, especially in seismology. The concepts of the most popular approaches are summarized. An own approach adapted to ultrasound signals and acoustic emissions, based on the Akaike Information Criterion (AIC), is presented. The AIC-picker is compared to an automatic onset detection algorithm based on the Hinkley criterion and also adapted to acoustic emissions. Manual picks performed by an analyst are used as reference values. Both automatic onset detection algorithms are applied to ultrasound signals which are used to monitor the setting and hardening of concrete. They are also applied to acoustic emissions recorded during a pull-out test. The AIC-picker produces sufficient reliable results for ultrasound signals where the deviation from the manual picks varies between 2% and 4%. Concerning acoustic emissions, only 10% of the events result in a mislocation vector greater than 5mm. It can be shown that our AIC-picker is a reliable tool for automatic onset detection for ultrasound signals and acoustic emissions of varying signal to noise ratio.     

In vitro ultrasonic and mechanic characterization of the modulus of elasticity of children cortical bone

The assessment of elastic properties in children’s cortical bone is a major challenge for biomechanical engineering community, more widely for health care professionals. Even with classical clinical modalities such as X-ray tomography, MRI, and/or echography, inappropriate diagnosis can result from the lack of reference values for children bone. This study provides values for elastic properties of cortical bone in children using ultrasonic and mechanical measurements, and compares them with adult values. 18 fibula samples from 8 children (5–16 years old, mean age 10.6 years old ±4.4) were compared to 16 fibula samples from 3 elderly adults (more than 65 years old). First, the dynamic modulus of elasticity (E_dyn) and Poisson’s ratio (ν) are evaluated via an ultrasonic method. Second, the static modulus of elasticity (E_sta) is estimated from a 3-point microbending test. The mean values of longitudinal and transverse wave velocities measured at 10 MHz for the children’s samples are respectively 3.2 mm/μs (±0.5) and 1.8 mm/μs (±0.1); for the elderly adults’ samples, velocities are respectively 3.5 mm/μs (±0.2) and 1.9 mm/μs (±0.09). The mean E_dyn and the mean E_sta for the children’s samples are respectively 15.5 GPa (±3.4) and 9.1 GPa (±3.5); for the elderly adults’ samples, they are respectively 16.7 GPa (±1.9) and 5.8 GPa (±2.1). E_dyn, ν and E_sta are in the same range for children’s and elderly adults’ bone without any parametric statistical difference; a ranking correlation between E_dyn and E_sta is shown for the first time.     

Periodic shock-emission from acoustically driven cavitation clouds: A source of the subharmonic signal

Single clouds of cavitation bubbles, driven by 254 kHz focused ultrasound at pressure amplitudes in the range of 0.48–1.22 MPa, have been observed via high-speed shadowgraphic imaging at 1 × 10⁶ frames per second. Clouds underwent repetitive growth, oscillation and collapse (GOC) cycles, with shock-waves emitted periodically at the instant of collapse during each cycle. The frequency of cloud collapse, and coincident shock-emission, was primarily dependent on the intensity of the focused ultrasound driving the activity. The lowest peak-to-peak pressure amplitude of 0.48 MPa generated shock-waves with an average period of 7.9 ± 0.5 μs, corresponding to a frequency of f₀/2, half-harmonic to the fundamental driving. Increasing the intensity gave rise to GOC cycles and shock-emission periods of 11.8 ± 0.3, 15.8 ± 0.3, 19.8 ± 0.2 μs, at pressure amplitudes of 0.64, 0.92 and 1.22 MPa, corresponding to the higher-order subharmonics of f₀/3, f₀/4 and f₀/5, respectively. Parallel passive acoustic detection, filtered for the fundamental driving, revealed features that correlated temporally to the shock-emissions observed via high-speed imaging, p(two-tailed) < 0.01 (r = 0.996, taken over all data). Subtracting the isolated acoustic shock profiles from the raw signal collected from the detector, demonstrated the removal of subharmonic spectral peaks, in the frequency domain. The larger cavitation clouds (>200 μm diameter, at maximum inflation), that developed under insonations of peak-to-peak pressure amplitudes >1.0 MPa, emitted shock-waves with two or more fronts suggesting non-uniform collapse of the cloud. The observations indicate that periodic shock-emissions from acoustically driven cavitation clouds provide a source for the cavitation subharmonic signal, and that shock structure may be used to study intra-cloud dynamics at sub-microsecond timescales.     

Laser scribing of indium tin oxide (ITO) thin films deposited on various substrates for touch panels

In this study, a Nd:YAG laser with wavelength of 1064 nm is used to scribe the indium tin oxide (ITO) thin films coated on three types of substrate materials, i.e. soda-lime glass, polycarbonate (PC), and cyclic-olefin-copolymer (COC) materials with thickness of 20 nm, 30 nm, and 20 nm, respectively. The effect of exposure time adjusted from 10 μs to 100 μs on the ablated mark width, depth, and electrical properties of the scribed film was investigated. The maximum laser power of 2.2 W was used to scribe these thin films. In addition, the surface morphology, surface reaction, surface roughness, optical properties, and electrical conductivity properties were measured by a scanning electron microscope, a three-dimensional confocal laser scanning microscope, an atomic force microscope, and a four-point probe. The measured results of surface morphology show that the residual ITO layer was produced on the scribed path with the laser exposure time at 10 μs and 20 μs. The better edge qualities of the scribed lines can be obtained when the exposure time extends from 30 μs to 60 μs. When the laser exposure time is longer than 60 μs, the partially burned areas of the scribed thin films on PC and COC substrates are observed. Moreover, the isolated line width and resistivity values increase when the laser exposure time increases.     

Controlled process for polymer micromachining using designed pulse trains of a UV solid state laser

A flexible workstation equipped with a solid state laser operating at 266 nm wavelength was used to machine holes in polyethylene terephthalate, polyimide and polycarbonate. An optical pulse picker was employed to reduce the high repetition rates of the laser, while a breakthrough sensor was used to avoid over-drilling of through holes. For each material, different repetition rates and designed pulse trains were tested to improve feature quality and process efficiency. Although the three polymers had very different reactions at this wavelength they all showed an improvement in feature quality with decreasing repetition rate due to a reduction in thermal effects. Up to 10 kHz the average depth per pulse remained unchanged and afterwards a slight increase was observed but this was accompanied by large uncertainties. Bursts of pulses at 40 kHz inserted inside the low repetition rate pulse train reduced the drilling time and the amount of debris redeposited without affecting the feature quality. It was found that a number of cleaning pulses after perforation eliminates the heat affected zone around exits. Holes with entrance diameters below 20 μm and exit diameters as small as 2 μm were obtained with high repeatability.     

Femtosecond and nanosecond laser damage thresholds of doped and undoped triazenepolymer thin films

The influence of pulse duration on the laser-induced damage in undoped or infrared-absorbing-dye doped thin triazenepolymer films on glass substrates has been investigated for single, near-infrared (800 nm) Ti:sapphire laser pulses with durations ranging from 130 fs up to 540 fs and complementarily for infrared (1064 nm) Nd:YAG ns-laser single-pulse irradiation. The triazenepolymer material has been developed for high resolution ablation with irradiation at 308 nm. Post-irradiation optical microscopy observations have been used to determine quantitatively the threshold fluence for permanent laser damage. In contrast to our previous studies on a triazenepolymer with different composition [J. Bonse, S.M. Wiggins, J. Solis, T. Lippert, Appl. Surf. Sci. 247 (2005) 440], a significant dependence of the damage threshold on the pulse duration is found in the sub-picosecond regime with values ranging from ∼500 mJ/cm² (130 fs) up to ∼1500 mJ/cm² (540 fs). Other parameters such as the film thickness (50 nm and 1.1 μm samples) or the doping level show no significant influence on the material behavior upon irradiation. The results for fs- and ns-laser pulse irradiation are compared and analyzed in terms of existent ablation models.     

Dual-high-frequency ultrasound excitation on microbubble destruction volume

Objective and motivation

The goal of this work was to test experimentally that exposing air bubbles or ultrasound contrast agents in water to amplitude modulated wave allows control of inertial cavitation affected volume and hence could limit the undesirable bioeffects.

Methods

Focused transducer operating at the center frequency of 10 MHz and having about 65% fractional bandwidth was excited by 3 μs 8.5 and 11.5 MHz tone-bursts to produce 3 MHz envelope signal. The 3 MHz frequency was selected because it corresponds to the resonance frequency of the microbubbles used in the experiment. Another 5 MHz transducer was used as a receiver to produce B-mode image. Peak negative acoustic pressure was adjusted in the range from 0.5 to 3.5 MPa. The spectrum amplitudes obtained from the imaging of SonoVue^TM contrast agent when using the envelope and a separate 3 MHz transducer were compared to determine their cross-section at the - 6 dB level.

Results

The conventional 3 MHz tone-burst excitation resulted in the region of interest (ROI) cross-section of 2.47 mm while amplitude modulated, dual-frequency excitation with difference frequency of 3 MHz produced cross-section equal to 1.2 mm.

Conclusion

These results corroborate our hypothesis that, in addition to the considerably higher penetration depth of dual-frequency excitation due to the lower attenuation at 3 MHz than that at 8.5 and 11.5 MHz, the sample volume of dual-frequency excitation is also smaller than that of linear 3-MHz method for more spatially confined destruction of microbubbles.     

Comparison of continuous-wave (CW) and tone-burst (TB) excitation modes in vibro-acoustography: Application for the non-destructive imaging of flaws

This paper describes the application of continuous-wave (CW) and tone-burst (TB) vibro-acoustography (VA) experiments for imaging a flawed composite plate. For both modes, the ultrasound frequency is set at f₁ = 3 MHz and f₂ = 3 MHz + ∣Δf∣. The plate was placed at the focus of the transducer and scanned point-by-point over an area of 60 mm by 50 mm on its frontal face with an increment step equal to 0.25 mm/pixel. The resulting acoustic emission amplitude at ∣Δ f∣ is recorded. For the CW mode the difference frequency was set at ∣Δf∣ = 12.9 kHz. For the TB mode, the burst-emitted signal was 100 μs long at a pulse repetition frequency (PRF) of 100 Hz corresponding to bursts of 300 cycles at 3 MHz, and the difference frequency was set at ∣Δf∣ = 44 kHz. The resulting VA images readily show the shape of the flaws. The images also reveal considerable detail of internal substructures such as the fibers used to reinforce the plate. However, the CW VA image shows an artifact caused by the effect of ultrasound standing waves established between the plate and the concave surface of the transducer, resulting in masking some of the flaws. On the other hand, the TB-VA image is free from such artifact. Despite some advantages of using TB-VA, there are some limitations related to this mode. Advantages and limitations of using the two modes are discussed.     

A combined multiple-SLED broadband light source at 1300 nm for high resolution optical coherence tomography

We demonstrate a compact, inexpensive, and reliable fiber-coupled light source with broad bandwidth and sufficient power at 1300 nm for high resolution optical coherence tomography (OCT) imaging in real-time applications. By combining four superluminescent diodes (SLEDs) with different central wavelengths, the light source has a bandwidth of 145 nm centered at 1325 nm with over 10 mW of power. OCT images of an excised stage 30 embryonic chick heart acquired with our combined SLED light source (<5 μm axial resolution in tissue) are compared with images obtained with a single SLED source (∼10 μm axial resolution in tissue). The high resolution OCT system with the combined SLED light source provides better image quality (smaller speckle noise) and a greater ability to observe fine structures in the embryonic heart.     

Graphene-based passively Q-switched 2 μm thulium-doped fiber laser

We demonstrated stable pulses generation at 2 μm in a passively Q-switched thulium-doped fiber laser using a few layer graphene thin film. The maximum output power was 4.5 mW and the single pulse energy was 85 nJ at 53 kHz repetition rate, and the pulse width was about 1.4 μs. The pulse width and the repetition rate of the Q-switched fiber laser can be changed along with the pump power. To the best of our knowledge, this is the first report of graphene saturable absorber for passively Q-switched 2 μm fiber lasers.     

Real-time monitoring of structural vibration using spectral-domain optical coherence tomography

We report in this paper the development of a spectral-domain optical coherence vibration tomography (OCVT) using a broadband CCD-based spectrometer and a short-coherence white light source. We demonstrate that both the vibration amplitude and frequency can be quantified, in the frequency range 0-250 Hz, with an axial resolution of 1 μm. Furthermore, the inner structure (layer thickness) of a vibrating sample can also be quantified simultaneously. The developed OCVT is non-contact and noninvasive in nature, thus is ideal for real time and in situ monitoring of low-frequency micro-vibrations that have critical impacts on many high-precision manufacturing and engineering processes.     

Influence of nesting shell size on brightness longevity and resistance to ultrasound-induced dissolution during enhanced B-mode contrast imaging

This study aims to bridge the gap between transport mechanisms of an improved ultrasound contrast agent (UCA) and its resulting behavior in a clinical imaging study. Phospholipid-shelled microbubbles nested within the aqueous core of a polymer microcapsule are examined for their use and feasibility as an improved UCA. The nested formulation provides contrast comparable to traditional formulations, specifically an SF₆ microbubble coated by a DSPC PEG-3000 monolayer, with the advantage that contrast persists at least nine times longer in a mock clinical, in vitro setting. The effectiveness of the sample was measured using a contrast ratio in units of decibels (dB) which compares the brightness of the nested microbubbles to a reference value of a phantom tissue mimic. During a 40 min imaging study, six nesting formulations with average outer capsule diameters of 1.95, 2.53, 5.55, 9.95, 14.95, and 20.51 μm reached final contrast ratio values of 0.25, 2.35, 3.68, 4.51, 5.93, and 8.00 dB, respectively. The starting contrast ratio in each case was approximately 8 dB and accounts for the brightness attributed to the nesting shell. As compared with empty microcapsules (no microbubbles nested within), enhancement of the initial contrast ratio increased systematically with decreasing microcapsule size. The time required to reach a steady state in the temporal contrast ratio profile also varied with microcapsule diameter and was found to be 420 s for each of the four smallest shell diameters and 210 s and 150 s, respectively, for the largest two shell diameters. All nested formulations were longer-lived and gave higher final contrast ratios than a control sample comprising un-nested, but otherwise equivalent, microbubbles. Specifically, the contrast ratio of the un-nested microbubbles decreased to a negative value after 4 min of continuous ultrasound exposure with complete disappearance of the microbubbles after 15 min whereas all nested formulations maintained positive contrast ratio values for the duration of the 40 min trial. The results are consistent with two distinct stages of gas transport: in the first stage, passive diffusion occurs under ambient conditions across the microbubble monolayer within the first few minutes after formulation until the aqueous interior of the microcapsule is saturated with gas; in the second stage ultrasound drives additional gas dissolution even further due to pressure modulation. It is important to understand the chemistry and transport mechanisms of this contrast agent under the influence of ultrasound to attain better perspicacity for enhanced applications in imaging. Results from this study will facilitate future preclinical studies and clinical applications of nested microbubbles for therapeutic and diagnostic imaging.     

Vibration characteristics of aluminum surface subjected to ultrasonic waves and their effect on wetting behavior of solder droplets

The vibration characteristics of an aluminum surface subjected to ultrasonic waves were investigated with a combination of numerical simulation and experimental testing. The wetting behavior of solder droplets on the vibrating aluminum surface was also examined. The results show that the vibration pattern of the aluminum surface is inhomogeneous. The amplitude of the aluminum surface exceeds the excitation amplitude in some zones, while the amplitude decreases nearly to zero in other zones. The distribution of the zero-amplitude zones is much less dependent on the strength of the vibration than on the location of the vibration source. The surface of the liquid solder vibrates at an ultrasonic frequency that is higher than the vibration source, and the amplitude of the liquid solder is almost twice that of the aluminum surface. The vibration of the surface of the base metal (liquid solder) correlates with the oxide film removal effect. Significant removal of the oxide film can be achieved within 2 s when the amplitude of the aluminum surface is higher than 5.4 μm or when the amplitude of the liquid solder surface is higher than 10.2 μm.