分光光度法同时测定人发中的钙,锌

将多元线性回归分析用于分光光度法同时测定人发中钙，锌元素的含量，讨论了该方法的原理，考察了最佳实验条件，建立了回归方程和线性方程。对６人模拟发样和２个人发样品进行了测定，结果令人满意。     

校正矩阵同时分光光度法测定人发中钙、镁、锌

将校正矩阵计算法应用于分光光度法中,同时测定了人发中的钙镁锌含量。讨论了方法原理和最佳实验条件,对４个发样和模拟样进行了测定,取得满意结果。     

极谱配合吸附波测定人发微量锰和铁

采用浓硝酸-高氯酸消化法结合锰铁-乙二胺极谱配合吸附波方法测定人发微量猛铁。对各种预处理方法进行了比较研究。测定了模拟人发、混合人发、国家一级标准物质人发样品以及某些肿瘤患者的发锰铁含量,均获得了满意结果。     

极谱配合吸附波法同时测定人发锌、铅、铜与评价

本文介绍用极谱配合吸附波方法同时快速测定人发中的锌、铅、铜,并着重研究了人发样品的预处理方法。对青岛市区各不同年龄组健康者与厌食症儿童发样测定的统计分析结果进行了评价。     

人发中锌、铜、铅含量的测定与评价

本文用极谱络合吸附波同时快速测定人发中的微量锌、铜、铅。测定了青岛市区不同年龄的人发样品336例,并对统计分析结果进行了评价。     

洗洁精-金试剂吸光光度法测定人发中微量铜

利用洗洁精为增敏、增溶剂试,以金试剂为显色剂,拟定了吸光光度法测定了人发中微量铜的方法,方法简便快速,准确度高,人发中常见离子不干扰铜的测定。     

人发中锌的光度分析近况

从光度法测定人发中Zn(Ⅱ）的重要显色剂出发,对近年来人发中Zn(Ⅱ）的光度测定进展作一综述。     

珠江三角洲肝癌高发区人发重金属元素来源及其影响因子分析

目的为解析珠江三角洲肝癌高发区人发重金属元素来源,探析其来源影响因子。方法利用电感耦合等离子体原子发射光谱仪对佛山顺德区人发的7种重金属元素Pb、Sr、Zn、Fe、Mg、Mn和Al的含量进行了测定,对人发元素进行多元统计分析。结果研究区居民人发Pb、Al和Fe的平均含量较人发正常值高,而人发Sr的平均含量均低于其它地区的正常值,Zn的平均含量在健康人群人发Zn含量平均值范围内。主成分分析提取的三个主成分可解释总方差的71%,Pb、Sr和Mg属外源性元素;Zn、Fe属内源性元素,可用于生物监测;Al、Mn既是外源性元素又是内源性元素。聚类分析验证了主成分分析的结果。不同年龄的人发元素含量,30岁以上的人发中Al平均含量经t检验显著高于30以下人群,其余元素均无显著差异;不同健康状况和性别的人发元素含量,各元素经统计检验均无显著差异。结论该地区人发中Pb、Al含量较健康人高,可能与肝癌高发有关,值得进步探讨其来源。     

发铅检测法的临床应用价值评估

从铅中毒发铅诊断标准、发铅诊断试验及发铅诊断临床应用3个方面论述了发铅检测法在铅中毒诊断、筛查和监督中的实际应用问题。根据临床经验和发铅-血铅比值确定了居民发铅正常值上限及铅中毒发铅诊断分级标准;即使以血铅测定值为"金标准",诊断试验表明,发铅测定在铅中毒诊断中仍有实际应用价值;发铅检测法自上世纪60年代以来一直沿用至今,绝大多数研究者认为,发铅测定是诊断、筛查铅中毒和监督环境铅污染最简单、有效的工具。     

塞曼原子吸收分光光度法测定人发样中痕量镍,铬,锰

提供了测定人发中微量金属元素Ｎｉ、Ｃｒ、Ｍｎ的塞慢原子吸收分析法，采用国产石墨管、空心阴极灯对分析条件进行了考察选择，并对人发标准参考物质进行测定。     

Stable (2)H isotope analysis of modern-day human hair and nails can aid forensic human identification

Continuous-flow isotope ratio mass spectrometry (CF-IRMS) was used to compare (2)H isotopic composition at natural abundance level of human scalp hair and fingernail samples collected from subjects worldwide with interpolated delta(2)H precipitation values at corresponding locations. The results showed a strong correlation between delta(2)H values of meteoric water and hair (r(2) = 0.86), while the corresponding correlation for nails was not as strong (r(2) = 0.6). Offsets of -180 per thousand and -127 per thousand were observed when calculating solutions of the linear regression analyses for delta(2)H vs. delta(18)O correlation plots of hair and nail samples, respectively. Compared with the +10 per thousand offset of the global meteoric water line equation these findings suggested that delta(18)O data from hair and nail would be of limited diagnostic value. The results of this pilot study provide for the first time tentative correlations of (2)H isotopic composition of human hair and nails with local water. Linear regression analyses for measured delta(2)H values of human hair and nails vs. water yielded delta(2)H(hair) = 0.49 x delta(2)H(water) - 35 and delta(2)H(nails) = 0.38 x delta(2)H(water) - 49, respectively. The results suggest that (2)H isotopic analysis of hair and nail samples can be used to provide information regarding an individual's recent geographical life history and, hence, location. The benefit of this technique is to aid identification of victims of violent crime and mass disasters in circumstances where traditional methods such as DNA and fingerprinting cannot be brought to bear (or at least not immediately).     

多元线性回归同时单点PM滴定法研究

本文将多元线性回归分析应用于单点ＰＭ滴定法中，同时测定了多组分金属离子混合物各组分浓度，讨论了方法原理和实验条件。对２８个二元、三元及四元混合样进行了测定，得到良好结果。     

出生缺陷高发区人发元素含量分析研究

目的:寻找出生缺陷高发区病人体内含量异常的元素,为出生缺陷的干预和治疗提供理论依据。方法:在病区分别采集有出生缺陷儿童和健康儿童头发样品,经预处理后,采用ICP-电感耦合等离子发射光谱法,对16种元素进行了测定,所得数据采用SPSS 10.0进行非参数检验和多元回归分析。结果:与健康儿童头发样品相比,患儿发中Mo,Zn,Sr,Fe,Mg,Ca等含量显著偏低,V偏高;以病情为因变量,发中16种元素含量为自变量经逐步回归分析,有Mo,Zn,Ni等进入方程。提示该区病人体内Mo,Zn等含量低、Ni含量高与病情有关。结论:Mo,Zn等在人体内的缺乏可能是出生缺陷发生的重要因素,Sr,K,Cu,Sn,Mg,Se,Fe,Ca等含量偏低及V含量偏高可能与Mo,Zn,Ni等共同起到协同致病的作用。     

2-(5-羧基-1,3,4-三氮唑偶氮)-5-二乙氨基苯甲酸-过氧化氢催化动力学光度法测定痕量铁的研究

研究了铁(Ⅲ)催化H2O2氧化2-(5-羧基-1,3,4-三氮唑偶氮)-5-二乙氨基苯甲酸(CTZDBA)的褪色反应及其动力学条件,褪色反应程度与铁(Ⅲ)量在一定范围内呈线性关系,建立了测定痕量铁(Ⅲ)的催化动力学光度法。在pH4.0的HAc-NaAc缓冲溶液介质中,褪色体系的最大吸收波长581nm,Fe(Ⅲ)的质量浓度在0.0008～0.04μg/mL范围内符合线性关系,回归方程为ΔA=25.325ρ-8.02×10-3(ρ:μg/mL),相关系数为0.9977,检出限3.78×10-10g/mL,方法用于头发和面粉中痕量铁的测定,结果与AAS测定结果相符。     

无锡出生缺陷高发区地理环境化学元素的特征

为了解出生缺陷高发区地理环境因素的异常特征,给出生缺陷的预防性干预提供科学依据,按照"无锡市出生缺陷病例/育龄妇女人口图"确定无锡市惠山区和锡山区为出生缺陷高发区,宜兴市为对照区。采用电感耦合等离子体原子发射光谱仪对采集的土壤、水、粮食、头发等515个样品进行了砷等17种元素的测定,利用SPSS 11.0软件对数据进行了非参数检验和多元线性回归分析。结果表明,无锡与山西出生缺陷高发区患者发样中钒含量均显著偏高;两个地区得到的回归方程中存在很多重叠元素,尤其是镁和钼几乎存在于每个方程中。可见钒含量显著偏高可能是导致出生缺陷发生的重要因素;镁和钼含量异常以及它们之间或与其它元素之间存在的协同或拮抗作用可能对出生缺陷的发生起到关键作用。     

阻抑-褪色光度法测定痕量硅

基于NH3 NH4 Cl缓冲溶液介质中 ,Si 对H2 O2 氧化十六烷基三甲基溴化铵 姜黄素离子缔合物的褪色反应的抑制作用 ,提出了测定痕量硅新的高灵敏的褪色光度法。该方法的表观摩尔吸光系数ε50 0=5 .70× 10 5L·mol-1·cm-1;线性范围为 0 .4～ 3.6 μg/L ;线性回归方程ΔA =2 .2 3× 10 -4 + 0 .0 2 0 17CSi (μg/L) ;相关系数r =0 .9999;测定下限为 2 0× 10 -8g/L。本法用于人发样和水样中痕量硅的测定 ,结果满意。     

Determination of Trace Titanium by Solid Substrate－Room Temperature Phosphorimetry with 4,5－Dibromophenylfluorone as Luminescent Ligand and Triton X－100 as Sensitizer

A new highly sensitive solid substrate room temperature phosphorimetry for the determination of trace titanium is proposed based on the sensitization of Triton X-100 to the SS-RTP intensity of 4, 5-dibro-mophenylfluorone-titanium complex adsorbed on the filter paper substrate modified by gelatin. When Triton X-100 was added into the luminescence system, the RTP intensity was raised 3 times stronger than that of the system without Triton X-100. The linear dynamic range of the new method is 0. 64 ～ 3.2 fg/spot (0. 4 μL) with a detection limit of 12.8 ag/spot, and the regression equation of the working curve is △Ip = 482. 0 119.5mTi（Ⅳ) (fg/spot), the correlation coefficient r= 0. 9992, n = 6. The phosphorescence lifetime (r= 0. 85 ms) was also determined. The recoveriesCand RSD) for the determinations of titanium in human hair and tea samples were 101.0% (3. 0%) and 99. 97% (4. 2%), respectively.     

Determination of trace aluminum by fluorescence quenching method based on catalysis of potassium chlorate oxidizing alizarin red

A new method for the determination of trace aluminum has been proposed. It is based on the fact that alizarin red can emit strong and stable fluorescence at 80 degrees C for 30 min and Al(3+) can effectively catalyze potassium chlorate oxidizing alizarin red to form non-fluorescence complex which cause the fluorescence quenching. The linear dynamic range of this method is 0.040-4.00 ngl(-1) with a detection limit of 5.3 pgl(-1). The regression equation can be expressed as DeltaI(f)=8.731+21.73c(Al(3+)) (ngl(-1)), with the correlation coefficient r=0.9992 (n=6). This sensitive, rapid and accurate method has been applied to the determination of trace aluminum(III) in human hair and tea samples successfully. What is more, the mechanism of catalyzing potassium chlorate oxidizing alizarin red by the fluorescence quenching method is also discussed.     

多元线性回归同时PH配位滴定法研究

本文将多元线性回归分析法应用于ＰＨ配位滴定，同时测定了与ＥＤＴＡ综合物稳定常数极为相摈金属离子浓度。讨论了方法原理、回归方程及最佳实验条件。对十个合成样进行了测定，得满意结果。     

Simultaneous quantification of tramadol and O‐desmethyltramadol in hair samples by gas chromatography–electron impact/mass spectrometry

Over recent years, hair has become the ideal matrix for retrospective investigation of chronic abuse, including for tramadol. However, in order to exclude the possibility of external contamination, it is also important to quantify simultaneously its main metabolite, O‐desmethyltramadol (M1), which presence in hair reflects systemic exposure. In the present study a methodology aimed at the simultaneous quantification of tramadol and M1 in human hair was developed and validated for the first time. After decontamination of hair samples (60 mg), tramadol and M1 were extracted with methanol in an ultrasonic bath (~5 h). Purification was performed by solid‐phase extraction using mixed‐mode extraction cartridges. Subsequently to derivatization, analysis was performed by gas chromatography–electron impact/mass spectrometry (GC‐EI/MS). The method proved to be selective. The regression analysis for both analytes was shown to be linear in the range of 0.1–20.0 ng/mg with correlation coefficients of 0.9995 and 0.9997 for tramadol and M1, respectively. The coefficients of variation oscillated between 3.85 and 13.24%. The limits of detection were 0.03 and 0.02 ng/mg, and the lower limits of quantification were 0.08 and 0.06 ng/mg for tramadol and M1, respectively. The proof of applicability was performed in hair samples from six patients undergoing tramadol therapy. All samples were positive for tramadol and M1. Copyright © 2013 John Wiley & Sons, Ltd.