Diterpenoids from Rabdosia nervosa

Six ent-kaurene type diterpenoids have been isolated from the leaves of Rabdosia nervosa. Among them, ganervosin A and B were new compounds, the structures of which were elucidated as 1 and 2 respectively by spectral and chemical methods. The other four were identified as odonicin (3), novelrabdosin (4), nodosin (5) and shikokianal acetate (6), Compounds 5 and 6 were for the first time isolated from this herb.     

Diterpenoids from Rabdosia Nervosa

Six ent-kaurene type diterpenoids were isolated from the leaves of Rabdosia nerwsa. Among them.ganer-vosin A and B are new compounds, the structures of which were elucidated as 1 and 2 respectively by spectral and chemical methods. The other four were identified as odonicin (3), novelrabdosin (4), nodosin (5) and shikokianal acetate (8). Compounds (1), (2), (5) and (8) have not been found in this herb previously.     

Abietane Quinones from Rabdosia serra

Rabdosiu Serra (M4-YId Hara is a chinese traditional medicine for the treatment ofhepatitis. enteritis. acute cholecystitis and dysentery'. The present communicationdescribes the first isolation of three abietane quinone diterpenoids from the herb (Figure1 ). The new abietane quinone, 16-acetoxy-7Q-ethoxyroyleanone I was elucidated. Inaddition. two known abietane quinones, horminone 2, 16-acetoxy-7-0-acetylhorminone 3and 6-sitosterol. stigmasterol. ursolic acid. palmitic acid were identifie…     

大萼香茶菜中的新二萜化合物

报道从大萼香茶菜叶的乙醇提取物中分离得到一种新的二萜类化合物, 并命名为大萼香茶菜癸素(1). 其药理活性实验结果证明, 该化合物对体外培养的Hela细胞有较强的抑制作用.     

冬凌草的化学成分研究

采用硅胶柱层析方法从冬凌草的全草乙醇提取物中分离了7个化合物,通过理化性质和波谱分析方法分别鉴定为豆甾醇(Ⅰ),β-谷甾醇(Ⅱ),冬凌草乙素(Ⅲ),lasiodon in(Ⅳ),冬凌草甲素(Ⅴ),5,3,′4′-三羟基-6,7-二甲氧基黄酮(Ⅵ)和胡麻素(Ⅶ).化合物Ⅰ为首次从该属植物中分得,化合物Ⅵ为首次从该植物中分得.     

A new diterpene glycoside from Rabdosia rubescens

A new ent-kaurene beta-D-glucoside was isolated from Rabdosia rubescens, together with the known compounds oridonin, ponicidin, and pedalitin. The structure of new compound was determined, on the basis of spectral data and X-ray crystallographic analysis, to be ent-7 beta,20-epoxy-kaur-16-ene-1 beta,6 alpha,7 alpha,14 alpha,15 alpha-pentanol 1-O-beta-D-glucopyranoside.     

皱叶香茶菜化学成分的研究

从采自西藏的皱叶香茶菜中分离鉴定了3对B-闭联对映贝壳杉烯型二萜化合物:二萜化合物皱叶香茶菜素(Ⅰ)和二氢皱叶香茶菜素(Ⅱ);isodocarpin(Ⅲ)和dihydroisodocarpin(Ⅳ)及Carpalasionin(Ⅴ)和dihydrocarpalasionin(Ⅵ)。(Ⅰ)、(Ⅲ)和(Ⅴ)分别与对应的二氢化合物(Ⅱ)、(Ⅳ)和(Ⅵ)以混合物的形式被分离和鉴定。化合物(Ⅱ)为新化合物,(Ⅰ)、(Ⅳ)和(Ⅵ)首次在自然界发现,(Ⅲ)和(Ⅴ)为已知化合物。     

Studies on the diterpenoid constituents from Rabdosia angustifolia

Two new ent-kaurene diterpenoids, angustifolin B and natural hydrogen-bonded 1:1 complex, angustifolin C, as well as four known diterpenoids, angustifolin, isodonal, sodoponin, tri-chorabdal B, were isolated from the leaves of Rabdosia angustifolia.     

Three New Diterpenoids from Rabdosia lophanthoides var. gerardiana

Three new diterpenoids, together with three known ones, were isolated from the air‐dried whole herbs of Rabdosia lophanthoides var. gerardiana. The structures of the new diterpenoids were established as 3,4‐dihydro‐11‐hydroxy‐10‐(1‐hydroxy‐1‐methylethyl)‐2,2,6‐trimethylnaphtho[1,8‐bc]oxocin‐5(2H)‐one ( 1 ), 11,12,15‐trihydroxyabieta‐5,8,11,13‐tetraen‐7‐one ( 2 ), (2R,3S,4S,4aR,8S,9aS,13aS,16aS)‐3,4,4a,8,9,9a,10,11,12,13,14,16a‐dodecahydro‐2‐(hydroxymethyl)‐6,6,10,10‐tetramethyl‐2H‐benzo[4,5]cyclohepta[1,2‐h]pyrano[2,3‐b][1,4]benzodioxepine‐3,4,8,13a,15(6H)‐pentol ( 3 ) by spectroscopic methods, including extensive 1D‐ and 2D‐NMR analyses. The structures of the known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature.     

Two new diterpenoids from Rabdosia japonica var.glaucocalyx

Two new diterpenoids, named glaucocalyxin F and glaucocalyxin X, respectively, were isolated from the whole plant of Rabdosia japonica （Burm. f.） Hara var. glaucocalyx （Maxim.） Hara. Their structures was established on the basis of spectral analysis.     

Ultrasound-assisted Extraction of Kamebakaurin from Rabdosia excisa by Response Surface Methodology

For the efficient extraction of kamebakaurin（KA）, the ultrasound-assisted extraction（UAE） of KA from Rabdosia excisa（R, excisa） via response surface methodology（RSM） was investigated with high-performance liquid chromatography（HPLC）. Effects of the experimental parameters such as extraction solvent, ratio of liquid to plant material, extraction time and extraction temperature on the extracting efficiency of KA from R. excisa were evaluated, and the purity of KA in residual was calculated. The optimized conditions were 65.5%（volume fraction） acetone, 35 ℃, time of 24.6 min with ultrasound of 80 W/L, 40 kHz, ratio of liquid to plant material at 30：1（mL/g）. The maximum yield of KA is 0.708 mg/kg, with mean purity of 6.09%, indicating that ultrasound-assisted extraction is a feasible and useful method for extracting KA from R. excisa.     

A new ent-kaurane diterpenoid from Rabdosia pseudo-irrorata C. Y. Wu

A new ent-kaurane diterpenoid, Pseurata H (1), was isolated from the aerial parts of Rabdosia pseudo-irrorata C. Y. Wu, along with three known compounds, Pseurata B (2), Pseurata C (3) and Pseurata F (4). Their structures were established by extensive spectroscopic techniques (MS, IR, 1D and 2D NMR experiments).     

Isolation and purification of oridonin from Rabdosia rubescens using upright counter-current chromatography

A preparative counter-current chromatography (CCC) method for isolation and purification of oridonin, a new cancer chemoprevention agent, from the Chinese medicinal plant Rabdosia rubescens was successfully established. The crude oridonin was obtained by elution with a light petroleum/acetone solvent mixture from ethanol extracts of R. rubescens using column chromatography on silica gel. With a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (1:2:1:2, v/v), 120 mg of oridonin at the purity of 97.8% was obtained from 200 mg of the crude sample in a single-step CCC separation. The structure of oridonin was identified by ESI-MS, 1H NMR, and 13C NMR.     

二甲戊乐灵在溪黄草及土壤中的残留分析方法研究

本文对溪黄草样品采用丙酮-正己烷提取,活性炭-中性氧化铝柱净化,石油醚-丙酮淋洗前处理;土壤样品经乙腈提取等步骤前处理,建立了溪黄草及土壤中二甲戊乐灵残留的气相色谱-电子捕获检测器(GC-ECD)的分析方法。结果表明:二甲戊乐灵的最小检出量为0.1ng,其在溪黄草和土壤中的最低检测浓度均为0.01mg/kg,在溪黄草中的平均回收率为88.53%~90.45%,相对标准偏差(RSD)为1.33%~2.76%;在土壤中的平均回收率为93.94%~96.23%,RSD为4.67%~4.80%,二甲戊乐灵在0.01~2mg/L范围内具有良好的线性关系。     

大萼香茶菜挥发油化学成分的气相色谱/质谱法分析

采用常规水蒸气蒸馏法提取出大萼香香茶菜挥发油，经气相色谱－质谱联机分析，分离出116个峰，确认了其中60种成分，所鉴定的组分占总峰面积的75．77％。     

Design and synthesis of antitumor compounds based on the cytotoxic diterpenoids from the genus Rabdosia

Two active sites responsible for antitumor activity, an oxirane ring and an alpha-methylene-cyclopentanone moiety, have been extracted from studies on the structure-activity relationship of the cytotoxic diterpenoids isolated from Rabdosia shikokiana. Series of the simplified cyclopentanone derivatives containing both of the two active sites in the molecule have been synthesized and evaluated for cytotoxicity against P 388 cells. The compounds possessing both of two active sites displayed cytotoxicity at a concentration of 1 microgram/ml, while those possessing a single active site showed no activity.     

溪黄草及其不同提取物的红外光谱研究

采用一维红外光谱、二阶导数谱和二维相关红外光谱对溪黄草原药材及其不同溶剂提取物进行了红外光谱研究。结果表明:溪黄草原药材中含有酯类、芳香类和糖苷类等物质,溪黄草水提物中主要为糖类物质,乙醇提取物中主要为黄酮类物质,石油醚提取物中主要为酯类物质。红外光谱能够快速、简便地提供中药及其提取物中主要化学成分的宏观信息,从而为后续的化学成分分析和中药提取分离方法的改进和优化提供有效参考。