Advanced tip enhanced Raman mapping (TERM) was applied to high resolution chemical identification on nanoscale. Thin poly(methyl methacrylate)/poly(styrene acrylonitrile) (SAN28/PMMA) blend films were measured at different stages of phase separation. New insights into the phase evolution behavior of the thin films were obtained, when the TERM images were compared. An unexpected morphology transition was observed after a few minutes annealing at 250 °C. No surface enrichment of PMMA was observed, differing from the previous reports on a similar well-studied system of SAN33/PMMA. The glass transition temperature, the surface and interfacial tension were found to be the main factors responsible for the phase evolution behavior of SAN28/PMMA films. 相似文献
The influence of dielectric substrates on the Raman scattering activities of Ag overlayers has been investigated. Materials
with low refractive indices, such as SiO2, SiOx and AlF3, were found to provide suitable supporting platforms for Ag films to give strong surface-enhanced Raman scattering for dye
molecules when illuminated at 488 nm. This finding was then extended to tip-enhanced Raman scattering (TERS). Huge enhancements
of 70–80×, corresponding to net enhancements of >104, were observed for brilliant cresyl blue test analyte when Ag-coated tips made from or precoated with low refractive index
materials were applied. The yield of fabricated tips that significantly enhance the Raman signals was found to be close to
100%. These findings provide crucial steps towards the use of TERS as a robust technique for rapid chemical imaging with nanometer
spatial resolution.
Figure Silver-coated dielectric tips for tip-enhanced Raman scattering (TERS) are capable of more than 10,000-fold enhancement 相似文献
The quality of the scanning tip is crucial for tip-enhanced Raman spectroscopy (TERS) experiments towards large signal enhancement and high spatial resolution. In this work, we report a controllable fabrication method to prepare TERS-active tips by modifying the tip apex at the atomic scale, and propose two important criteria to in-situ judge the tip's TERS activity for tip-enhanced Raman measurements. One criterion is based on the downshift of the first image potential state to monitor the coupling between the far-field incident laser and near-field plasmon; the other is based on the appearance of the low-wavenumber Raman peaks associated with an atomistic protrusion at the tip apex to judge the coupling efficiency of emissions from the near field to the far field. This work provides an effective method to quickly fabricate and judge TERS-active tips before real TERS experiments on target molecules and other materials, which is believed to be instrumental for the development of TERS and other tip-enhanced spectroscopic techniques. 相似文献
Summary: The thermosensitive phase‐separation of poly(vinyl methyl ether) in water has been investigated by micro‐Raman spectroscopy in the presence of tetraalkylammonium bromides. The equilibrium distribution of both polymer and salts to the polymer‐rich and solvent‐rich phases was determined as a function of temperature. Tetraalkylammonium ions with longer alkyl chains associate more strongly with PVME and raise the phase‐transition temperature due to an increase in hydrophilicity.
Surface-enhanced Raman scattering (SERS) has established itself as an important analytical technique. However, efforts to
transfer the technology from the laboratory to the production line, clinic or field have been frustrated by the lack of robust
affordable substrates and the complexity of interfacing between sample and spectrometer. Prompted by the success of optical
fibre systems for implementing normal Raman scattering spectroscopy in remote locations and biomedical applications, attention
has now shifted to the development of SERS-active optical fibres. Other workers have attempted to develop SERS probes with
extended interaction lengths and both far-field and near-field SERS imaging techniques for high-resolution chemical mapping
of surfaces. This review discusses the development of these technologies and presents the current state of the art. Although
recent developments show great promise, some outstanding challenges and opportunities remain to be addressed. 相似文献
This study examines the feasibility of using tip-enhanced Raman spectroscopy (TERS) for label-free chemical characterization
of nanostructures in biological systems. For this purpose, a well-defined model system consisting of calcium alginate fibers
is studied. In a companion paper, calcium alginate fibers and their network structures were shown to be a good model for the
extracellular polysaccharides of biofilms at the nanoscale. TERS analysis of biological macromolecules, such as alginates,
is complicated by heterogeneity in their sequence, molecular weight, and conformations, their small Raman cross-section, and
the large number of functional groups, which can chemically interact with the silver surface of the tip and cause significant
band shifts. Due to these effects, Raman frequencies in TERS spectra of biopolymers do not necessarily resemble band positions
in the normal Raman spectrum of the bulk material, as is the case for less complex samples (e.g., dye molecules) studied so
far. Additionally, analyte decomposition due to laser heating can have a significant influence, and carbon contamination signals
can sometimes even overwhelm the weak analyte signals. Based on the investigation of alginates, strategies for spectra correction,
choice of appropriate reference samples, and data interpretation are presented. With this approach, characteristic frequency
ranges and specific marker bands can be found for biological macromolecules that can be employed for their identification
in complex environments.
Figure TERS spectrum of a calcium alginate fiber bundle 相似文献
The dye nuclear fast red has been detected and determined semi-quantitatively by means of surface enhanced resonance Raman scattering (SERRS) and surface enhanced Raman scattering (SERS), using laser exciting wavelengths of 514.5 and 632.8 nm, respectively, by employing a citrate-reduced silver colloid. A good linear correlation is observed for the dependence of the intensities of the SERRS bands at 989 cm−1 (R=0.9897) and 1278 cm−1 (R=0.9872) on dye concentration over the range 10−9 to 10−7 M, when using an exciting wavelength of 514.5 nm. At dye concentrations above 10−7 M, the concentration dependence of the SERRS signals is non-linear. This is almost certainly due to the coverage of the colloidal silver particles being in excess of a full monolayer of the dye. A linear correlation is also observed for the dependence of the intensities of the SERS bands at 989 cm−1 (R=0.9739) and 1278 cm−1 (R=0.9838) on the dye concentration over the range 10−8 to 10−6 M when using an exciting wavelength of 632.8 nm. Strong fluorescence prevented collection of resonance Raman scattering (RRS) spectra from powdered samples or aqueous solutions of the dye using an exciting wavelength of 514.5 nm, but weak bands were observed in the spectra obtained from both powdered and aqueous samples of the dye using an exciting wavelength of 632.8 nm. A study of the pH dependence of SERRS/SERS and UV–VIS absorption spectra revealed the presence of different ionisation states of the dye. The limits of detection for nuclear fast red by SERRS (514.5 nm), SERS (632.8 nm) and visible spectroscopy (535 nm) are 9, 89 and 1000 ng ml−1, respectively. 相似文献
Methods for chemical analysis at the nanometer scale are crucial for understanding and characterizing nanostructures of modern materials and biological systems. Tip‐enhanced Raman spectroscopy (TERS) combines the chemical information provided by Raman spectroscopy with the signal enhancement known from surface‐enhanced Raman scattering (SERS) and the high spatial resolution of atomic force microscopy (AFM) or scanning tunneling microscopy (STM). A metallic or metallized tip is illuminated by a focused laser beam and the resulting strongly enhanced electromagnetic field at the tip apex acts as a highly confined light source for Raman spectroscopic measurements. This Review focuses on the prerequisites for the efficient coupling of light to the tip as well as the shortcomings and pitfalls that have to be considered for TERS imaging, a fascinating but still challenging way to look at the nanoworld. Finally, examples from recent publications have been selected to demonstrate the potential of this technique for chemical imaging with a spatial resolution of approximately 10 nm and sensitivity down to the single‐molecule level for applications ranging from materials sciences to life sciences. 相似文献
A complicated thermochromic phase transition (PT) of order—disorder type in poly(di-n-decylsilane) has been studied in detail by variable-temperature UV and Raman spectroscopy. Not less than five polymer modifications were shown to participate in this PT. Above the equilibrium PT temperature Tc (60 °C), the polymer exists as a hexagonal columnar mesophase (HCM) with a disordered silicon backbone and disordered side chains. PT proceeds not abruptly but over a temperature range 60—5 °C and does not reach completion, being stopped by glassification. At room temperature, a coexistence in the polymer of HCM and a few crystalline phases with ordered backbone is observed. The latter are separated in space, what was evidenced by Raman micromapping of a polymer film. The temperature intervals of existence of different ordered phases also do not coincide, this enables one to identify in the UV and Raman spectra the components, corresponding to the modifications possessing trans (anti) and, supposedly, AD+AD– main chain conformations. As the relaxation processes in this comb-like polymer occur slowly, the quantitative ratio of different phases is not a single-valued function of temperature but depends on the thermal prehistory of the sample. Ordering of long side chains on cooling was demonstrated by the Raman method. 相似文献
Capillary elcctrophoresis (CE) was used for the qualitative and quantitative analysis of eleven compounds of the carbamate, thio-carhamate, and dithiocarbamate classes of pesticides. Micellar elec-trokinetic chromatography (MEKC) was employed for the separation of these substances. The analysis was performed using an uncoated fused silica column, in borate buffer containing SDS and 10% methanol at basic pH, and UV detection at 230 nm. The addition of methanol to the buffer increased the separation and affected intrinsic migration of analytes. The working standard solutions were prepared in acetonitrile/water 50:50. Although these compounds are structurally different, with a large spectrum of chemical properties, such as polarity and solubility, good separation and sensitivity were obtained. Moreover, good recovery of car-bamates was obtained from tap-water using a styrene-divinylben-zene polymer column with the solid phase extraction (SPE) technique. 相似文献