CdS量子点与曙红Y间的荧光共振能量转移研究

在水溶液中,量子点与有机荧光染料之间可能发生荧光共振能量转移(FRET)。本文以发射波长470nm的Cd S量子点为供体,曙红Y为受体,建立了Cd S量子点-曙红Y的FRET体系,研究了该体系的FRET参数。该体系受体供体数目比为8,猝灭效率为45.6%,增强效率为20.1%;供体-受体间的距离为4.4nm;临界能量转移距离为2.4nm。     

CdTe量子点-罗丹明6G荧光共振能量转移体系的构建及其应用研究

采用巯基化合物修饰的CdTe量子点构建了量子点(供体)-罗丹明6G(受体)荧光共振能量转移体系, 研究了CdTe量子点与牛血清白蛋白(BSA)的相互作用. 结果表明, CdTe量子点与BSA相互作用后提高了CdTe量子点-罗丹明6G 体系的荧光共振能量转移(FRET)效率, 减小了CdTe量子点和罗丹明6G分子间的距离(r), 证实BSA是通过其色氨酸(Trp)残基与CdTe量子点表面金属发生配位作用而直接结合到量子点表面的.     

CdTe量子点与罗丹明6G间的荧光共振能量转移机理研究

本文在合成水溶性巯基乙酸修饰的CdTe量子点的基础上,研究了CdTe量子点与罗丹明6G之间的荧光共振能量转移.实验结果表明:构建的CdTe量子点(供体)-罗丹明6G(受体)荧光共振能量转移体系在磷酸盐缓冲溶液中有较好的转移效果.当磷酸缓冲溶液pH值为7.4,NaCl浓度为1.0 mol/L时,构建的CdTe量子点-罗丹...     

量子点-罗丹明B比率型荧光探针的制备与性能

本文采用CdSe/Cd_xZn_(1-x)S核/合金壳量子点(QDs)作为荧光共振能量转移体系(FRET)的供体,通过巯基络合作用在其表面修饰一层L-半胱氨酸(Cys)分子,赋予QDs优异的水溶性能,再通过静电相互作用将罗丹明B(RhB)构筑于QDs-Cys表面,获得了一类新型的水溶性FRET体系.采用荧光光谱分析了pH以及供受体浓度比对FRET能量转移效率的影响.研究结果表明,通过静电结合构筑的QDs-Cys-RhB荧光共振能量转移体系对pH和供受体浓度具有敏感的荧光信号响应性能.当pH从10降到7时,FRET体系的荧光共振能量转移效率由49.39%增加到58.99%;当供受体浓度比为3:1时,FRET体系的能量转移效率高达61.09%.由此可见,通过表面络合与静电相互作用构筑的QDs-Cys-RhB荧光共振能量转移体系具有优异的光信号响应性,可以作为一类灵敏、精确的可调式比率型荧光探针,在生物检测、免疫分子等领域中具有广阔的应用前景.     

CdTe量子点与罗丹明B间的荧光共振能量转移研究

以巯基乙酸为稳定剂, 在水溶液中合成了CdTe量子点. 以发射波长522 nm的量子点为给体, 罗丹明B为受体, 研究了在十六烷基三甲基溴化铵胶束介质中, 量子点与罗丹明B之间的荧光共振能量转移. 实验结果表明, 在pH＝5.00的缓冲溶液中, 当十六烷基三甲基溴化铵的浓度为1.37×10－4 mol/ L时, 量子点与罗丹明B之间的距离为2.65 nm, 1个量子点给体最多可与6个罗丹明B受体发生有效的共振能量转移, 能量转移效率为0.69.     

CdTe/CdS-罗丹明荧光共振能量转移猝灭法测定铅(Ⅱ)及相互作用机制的研究

以巯基乙酸为修饰剂,水相合成量子产率高达75％的CdTe/CdS核壳型量子点,建立了以CdTe/CdS QDs作为能量供体,罗丹明B作为能量受体组成的荧光共振能量转移体系.在此基础上,以CdTe/CdS-罗丹明B为荧光探针,荧光共振能量转移猝灭为理论基础,设计出一种检测pb2+含量的方法.在0～9.62×10-11mo...     

基于Ⅰ型核壳量子点的宽光谱响应的高效能量转移体系

针对常规F?rster共振能量转移(FRET)体系中能量转移效率低的问题,合成了可见光吸收的Ⅰ型CIS@Zn S核-壳量子点作为能量供体,近红外方酸(SQ)染料作为能量受体,采用超声自组装的方式首次制备了光谱匹配、间距可调的高效FRET能量转移体系.超快/时间分辨光谱证明了CIS和SQ之间的FRET能量转移机制:CIS*+SQ→CIS+SQ*.荧光猝灭动力学数据显示,CIS@Zn S与SQ之间的能量转移对量子点的尺寸存在依赖性,由CIS@Zn S尺寸增加引起的荧光量子产率和供体-受体间距的增加使得体系的FRET能量转移效率(ηFRET)先增大后减小,并且在壳层反应时间为20min时体系的ηFRET值达到最佳值62.8%.该研究对于开发新型、高效、全谱响应的太阳能电池将具有一定的理论及实际应用价值.     

基于CdTe量子点荧光猝灭法测定水体中结晶紫

以巯基乙酸为稳定剂,用水热法合成CdTe量子点(QDs),基于结晶紫对CdTe QDs的荧光猝灭作用,建立一种新的测定结晶紫含量的新方法。在优化实验条件下,结晶紫浓度在1.0~10.0μmol·L-1范围内与CdTe QDs的荧光猝灭程度呈良好的线性关系,相关系数r=0.9986,检出限为0.026μmol·L-1。该方法用于水样中结晶紫含量的测定,加标回收率为96.2%~103.1%。同时,对结晶紫和CdTe QDs之间的反应机理进行探讨,发现CdTe QDs的发射光谱与结晶紫的吸收光谱能够有效重叠,且二者通过静电作用结合,可建立以CdTe QDs为供体,结晶紫为受体的荧光共振能量转移体系。     

基于甲基红-CdTe量子点荧光共振能量转移体系测定痕量强力霉素

本文以甲基红(MR)为能量给体,CdTe量子点(QDs)为能量受体,构建了MRCdTe QDs荧光共振能量转移体系,同时基于荧光猝灭法建立了检测痕量强力霉素(DOX)的新方法。实验表明,在pH=8.0的Tris-HCl缓冲介质中,聚乙烯醇(PVA)胶束能缩短MR与CdTe QDs分子间的距离,二者之间发生有效能量转移,使CdTe QDs荧光强度增强。而强力霉素能够猝灭该体系中CdTe QDs的荧光强度,以此建立了能量转移荧光猝灭法测定痕量强力霉素的新方法。实验表明强力霉素的线性范围为8~350×10-9 mol/L,检出限为2.1×10-9 mol/L。方法可应用于实际样品中强力霉素残留的测定。     

基于I型核壳量子点的宽光谱响应的高效能量转移体系

针对常规Förster共振能量转移(FRET)体系中能量转移效率低的问题, 合成了可见光吸收的I型CIS@ZnS核-壳量子点作为能量供体, 近红外方酸(SQ)染料作为能量受体, 采用超声自组装的方式首次制备了光谱匹配、间距可调的高效FRET能量转移体系. 超快/时间分辨光谱证明了CIS和SQ之间的FRET能量转移机制: CIS*+SQ→CIS+SQ*. 荧光猝灭动力学数据显示, CIS@ZnS与SQ之间的能量转移对量子点的尺寸存在依赖性, 由CIS@ZnS尺寸增加引起的荧光量子产率和供体-受体间距的增加使得体系的FRET能量转移效率(η_FRET)先增大后减小, 并且在壳层反应时间为20 min时体系的η_FRET值达到最佳值62.8%. 该研究对于开发新型、高效、全谱响应的太阳能电池将具有一定的理论及实际应用价值.     

Furyl- and thienyl-silatranes and germatranes

We review our research on the synthesis and study of the physical and biological properties of furyl- and thienylgermatranes and -silatranes.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 6, pp. 725–732, June, 1992.     

Review and patents and literature

The use of the insect cell/baculovirus expression system for producing recombinant proteins of bacterial, plant, insect, and mammalian origin has become widespread. The popularity of this eukaryotic expression system is due to many factors, including (1) potentially high protein expression levels, (2) ease and speed of genetic engineering, (3) ability to accommodate large DNA inserts, (4) protein processing similar to higher eukaryotic cells (e.g., mammalian cells), and (5) ease of insect cell growth (e.g., suspension growth). The following review of the literature discusses two engineering aspects of recombinant protein synthesis by insect cell cultures: bioreactor scale-up and insect cell line selection. Following this review patent abstracts and additional literature pertaining to expression of recombinant proteins in insect cell culture are listed.     

Ground- and excited-state aromaticity and antiaromaticity in benzene and cyclobutadiene

The aromaticity and antiaromaticity of the ground state (S 0), lowest triplet state (T 1), and first singlet excited state (S 1) of benzene, and the ground states (S 0), lowest triplet states (T 1), and the first and second singlet excited states (S 1 and S 2) of square and rectangular cyclobutadiene are assessed using various magnetic criteria including nucleus-independent chemical shifts (NICS), proton shieldings, and magnetic susceptibilities calculated using complete-active-space self-consistent field (CASSCF) wave functions constructed from gauge-including atomic orbitals (GIAOs). These magnetic criteria strongly suggest that, in contrast to the well-known aromaticity of the S 0 state of benzene, the T 1 and S 1 states of this molecule are antiaromatic. In square cyclobutadiene, which is shown to be considerably more antiaromatic than rectangular cyclobutadiene, the magnetic properties of the T 1 and S 1 states allow these to be classified as aromatic. According to the computed magnetic criteria, the T 1 state of rectangular cyclobutadiene is still aromatic, but the S 1 state is antiaromatic, just as the S 2 state of square cyclobutadiene; the S 2 state of rectangular cyclobutadiene is nonaromatic. The results demonstrate that the well-known "triplet aromaticity" of cyclic conjugated hydrocarbons represents a particular case of a broader concept of excited-state aromaticity and antiaromaticity. It is shown that while electronic excitation may lead to increased nuclear shieldings in certain low-lying electronic states, in general its main effect can be expected to be nuclear deshielding, which can be substantial for heavier nuclei.     

多环芳二酐型聚酯亚胺膜的透气性能

多环芳二酐型聚酯亚胺膜的透气性能李悦生，丁孟贤，徐纪平（浙江大学高分子科学与工程研究所，杭州，３１００２７）（中国科学院长春应用化学研究所）关键词聚醚酰亚胺，聚酯酰亚胺，膜，透气性通常的聚酰亚胺加工性能较差，在芳环二酐的苯环间引入醚键等柔性基团后，其...     

Determination and study on residue and dissipation of benazolin-ethyl and quizalofop-p-ethyl in rape and soil

A QuEChERS (quick, easy, cheap, effective, rugged, and safe) method for the determination of benazolin-ethyl and quizalofop-p-ethyl in rape and soil by high-performance liquid chromatography-tandem mass spectrometry has been developed in this study. The residue and dissipation of benazolin-ethyl and quizalofop-p-ethyl in rape and soil were determined with the developed method. The half-lives of benazolin-ethyl in rape straw and soil were 3.7–5.1 days and 14.3–26.3 days, respectively. The half-lives of quizalofop-p-ethyl in rape straw and soil were 5.0-6.1 days and 0.3–9.7 days, respectively. The residue of benazolin-ethyl and quizalofop-p-ethyl in rapeseed and soil were below the detection limit (i.e., 0.5?mg?kg^?1, the maximum residue level of European Union for quizalofop-p-ethyl).