CCl2(A1B1)被O2及取代甲烷类分子猝灭的动力学研究

对CCl4／Ar混合气体放电产生CCl2自由基，再用541.52nm激光将电子基态CCl2激发到激发态A^1B1（0，4，0）振动态k=0能级上，通过检测 激发态CCl2时间分辨荧光信号，测得室温下CCl2(A^1B1)被O2，CF4，CF2Cl，CH3NO2，CH2Br2等分子猝灭的实验结果，用改进的三能级模型分析处理实验数据，获得态分辨速常数KA和Ka值，并对实验结果进行了讨论。     

CCl2(A^1B1)自由基不同振动态的猝灭动力学

对CCl4/Ar混合气体直流脉冲放电产生CCl2自由基，再分别用波长为550.56nm,541.52nm,532.25nm,524.31nm,523.82nm和523.27nm的激光将电子基态CCl2激励到激发态A^1B1的(0,3,0),(0,4,0),(1,3,0),(0,6,0),(1,4,0),(2,2,0)振动态，激发态CCl2(A^1B1)的不同振动态的时间分辨荧光信号显示该信号呈双指数衰减，测得室温下CCl2(A^1B1)不同振动态被NH3,H2O,CH2Br2,NH(CH3)2,NH(C2H5)2,N(C2H5)3,n-C6H14等分子猝灭的实验结果，用三能级模型分析处理实验数据，获得态分辨速率常数kA和ka值，并对实验结果进行了讨论。     

CCl2(A1B1和a3B1)被酮类分子猝灭速率常数的测定

对CCl4/Ar混合气体脉冲直流高压放电产生CCl2自由基,经过约110μs后,再用541.52mm激光将电子基态CC2激励到激发态A1B1(0,4,0)振动态K=0能级上,通过检测激发态CCl2(A)时间分辨荧光信号,测得室温下CC2(A1B1和a3B1)被酮类分子猝灭的实验结果,用所提出的三能级模型分析处理实验数据,获得态分辨速率常数KA和Ka值.     

CCl2(A 1B 1,a3B1)自由基被烷烃类分子猝灭动力学

用放电 LIF实验装置,对CCl4/Ar混合气体放电产生CCl2自由基,再用541.52 nm激光将电子基态CCl2激励到激发态A 1B1(0,4,0)振动能级上,通过检测激发态CCl2时间分辨荧光信号,测得室温下CCl2(A 1B1)被烷烃类分子猝灭的实验结果,用我们提出的三能级模型分析处理实验数据,获得CCl2(A 1B1)态和CCl2(a 3B1)态的碰撞猝灭速率常数kA和ka值.     

CCl2(A1B1)被甲烷及氯代甲烷猝灭动力学

用放电-LIF实验装置,对CCl4/Ar混合气体放电产生*CCl2,再用541.52nm激光将电子基态*CCl2激发到激发态A1B1(0,4,0)模pR1能级上,检测激发态*CCl2时间分辨荧光信号,测得室温下*CCl2(A1B1)被甲烷及氯代甲烷分子猝灭的实验结果,用三能级模型分析处理实验数据获得态分辨速率常数kq1,kq2和kq3值和*CCl2(A1B1→X1A1)及(a3B1→X1A1)自发辐射寿命分别为(0.83±0.03)μs和(3.12±0.25)μs.     

脉冲直流放电-LIF法CCl2(A1B1和a3B1)自由基被醇类分子猝灭动力学研究

CCl4/Ar混合气体进行脉冲高压直流放电产生CCl2自由基,再用YAG泵浦的染料激光输出的541.52 nm激光将电子基态CCl2激励到电子激发态A1B1(0,4,0)模的pR1能级上,通过检测激发态CCl2自由基的时间分辨荧光信号,测得了室温下(293 K)激发态CCl2自由基被醇类分子猝灭的动力学结果,根据我们提出的三能级模型来分析处理这些实验数据,首次获得了激发态CCl2自由基被醇类分子猝灭的态分辨的速率常数kq1,kq2和kq3的值.并对这些数据与猝灭剂的结构关系进行了分析讨论.     

CCl2自由基被无机小分子猝灭的速率常数

对CCl4/Ar混合气体脉冲直流高压放电产生CCl2自由基,用541.52 nm激光将电子基态CCl2激励到A 1B1(0,4,0)振动能级上. 通过检测激发态CCl2时间分辨荧光信号,测得室温下CCl2(A 1B1和a 3B1)被O2、N2、NO、 CO2、 CS2、H2O、SO2,和SF6分子猝灭的实验结果.用我们提出的三能级模型分析处理实验数据,获得了CCl2(A 1B1)态和CCl2(a 3B1)态的碰撞猝灭速率常数kA和ka值.     

CCl_2(A~1B_1,a~3B_1)自由基被烷烃类分子猝灭动力学

用放电- LIF实验装置 ,对 CCl4/Ar混合气体放电产生 CCl_2自由基 ,再用 541.52 nm激光将电子基态 CCl_2激励到激发态 A~B_1(0,4,0)振动能级上 ,通过检测激发态 CCl_2时间分辨荧光信号 ,测得室温下 CCl_2(A~B_1)被烷烃类分子猝灭的实验结果 ,用我们提出的三能级模型分析处理实验数据 ,获得 CCl_2(A~1B_1)态和 CCl_2(a~3B_1)态的碰撞猝灭速率常数 k_A和 k_a值 .     

脉冲直流放电-LIF法CCl~2(A'B~1和a^3B~1)自由基被醇类分子 猝灭动力学研究

CCl~4/Ar混合气体进行脉冲高压直流放电产生CCl~2自由基,再用YAG泵浦的染料激光输出的541.52nm激光将电子基态CCl~2激励到电子激发态A^1B~1(0,4,0)模的P^R~1能级上,通过检测激发态CCl~2自由基的时间分辨荧光信号,测得了室温下(293K)激发态CCl~2自由基被醇类分子猝灭的动力学结果,根据我们提出的三能级模型来分析处理这些实验数据,首次获得了激发态CCl~2自由基被醇类分子猝灭的态分辨的速率常数k~q~1,k~q~2和k~q~3的值,并对这些数据与猝灭剂的结构关系进行了分析讨论。     

脉冲直流放电-LIF法CCl~2(A'B~1和a^3B~1)自由基被醇类分子 猝灭动力学研究

CCl~4/Ar混合气体进行脉冲高压直流放电产生CCl~2自由基,再用YAG泵浦的染料激光输出的541.52nm激光将电子基态CCl~2激励到电子激发态A^1B~1(0,4,0)模的P^R~1能级上,通过检测激发态CCl~2自由基的时间分辨荧光信号,测得了室温下(293K)激发态CCl~2自由基被醇类分子猝灭的动力学结果,根据我们提出的三能级模型来分析处理这些实验数据,首次获得了激发态CCl~2自由基被醇类分子猝灭的态分辨的速率常数k~q~1,k~q~2和k~q~3的值,并对这些数据与猝灭剂的结构关系进行了分析讨论。     

Development of a multiplex UPLC-MRM MS method for quantification of human membrane transport proteins OATP1B1, OATP1B3 and OATP2B1 in in vitro systems and tissues

OATP1B1, OATP1B3 and OATP2B1 are important members of the organic anion transporting polypeptides (OATP) family and are implicated in the hepatic disposition of endobiotics and xenobiotics. Quantitating the expression levels of human OATP1B1, OATP1B3 and OATP2B1 in in vitro systems and tissue samples could significantly improve attempts to scale up in vitro data and result in more effective in vitro–in vivo correlation of transporter-mediated effects on drug disposition, such as hepatic clearance. In the present study, a quantification method was developed, characterized, and implemented for simultaneous determination of human OATP1B1, OATP1B3 and OATP2B1 in HEK cells transfected with OATP-expressing plasmid vectors (SLCO1B1, SLCO1B3, and SLCO2B1, respectively), human hepatocytes, human brain capillary endothelial cells, and humanized mouse liver tissue using UPLC-MRM MS. Purified membrane protein standards prepared and characterized as previously reported (Protein Expr. Purif. 2008, 57, 163-71) were first used as standards for absolute quantification of the expression levels of the three human OATP membrane proteins. The specificity of the optimized MRM transitions were characterized by analyzing the tryptic digests of the membrane protein fraction of wild type HEK cells and control mouse liver tissue using the herein reported UPLC-MRM MS method. The linearity of the calibration curve spanned from 0.2 μg mL⁻¹ (0.040 μg mg⁻¹) to 20 μg mL⁻¹ (4.0 μg mg⁻¹), with accuracy (% RE) within 15% at all concentrations examined for all three OATPs of interest in this study. The intra- and inter-day assay accuracy (% RE) and coefficient of variations (% CV) of triplicates are all within 15% for all levels of quality control samples prepared by mixing the membrane fraction of control mouse liver tissue with the required amount of purified human OATP1B1, OATP1B3 and OATP2B1.     

CCl_2自由基被无机小分子猝灭的速率常数

对ＣＣｌ４／Ａｒ混合气体脉冲直流高压放电产生 ＣＣｌ２自由基,用 ５４１．５２ ｎｍ激光将电子基态 ＣＣｌ２激励到Ａ１Ｂ１（０,４,０）振动能级上．通过检测激发态 ＣＣｌ２时间分辨荧光信号,测得室温下 ＣＣｌ２（Ａ１Ｂ１和 ａ３Ｂ１）被 Ｏ２、Ｎ２、ＮＯ、ＣＯ２、ＣＳ２、Ｈ２Ｏ、ＳＯ２,和ＳＦ６分子猝灭的实验结果．用我们提出的三能级模型分析处理实验数据,获得了ＣＣｌ２（Ａ１Ｂ１）态和 ＣＣｌ２（ａ３Ｂ１）态的碰撞猝灭速率常数 ｋＡ和 ｋａ值．     

Syntheses and Bioactivity of 4"-Sulfonate-5-triphenylsilyl Avermectin B1a and Ivermectin B1a Derivatives

Fourteen new derivatives of avermectin B1a and ivermectin B1a were synthesized from C5-O-triphenylsilyl avermectin B1a and ivermectin B1a(yield from 40% to 83%). Their chemical structures were characterized by means of IR, 1H NMR, 13C NMR and FAB-MS spectrometries. Some of them show excellent insecticidal activity.     

Syntheses and Bioactivity of 4＂-Sulfonate-5-triphenyl silyl Avermectin B1a and Ivermectin B1a Derivatives

Fourteen new derivatives of avermectin B1a, and ivermectin B1a were synthesized from C5-O-triphenyl silylavermectin B1a and ivermectin B1a (yield from 40% to 83%). Their chemical structures were characterized by means of IR. ^1H NMR, ^13C NMR and FAB-MS spectrometries. Some of them show excellent insecticidal activity.     

长效维生素B1-优硫胺的合成研究

作者报道了合成长效维生素Ｂ１—优硫胺的新方法,收率达到７９．８％。     

FI-化学发光法间接测定维生素B1

提出利用Na2SO3的歧化反应与Luminol构建化学发光体系,并对其反应机理作了探讨.试验发现,Na2SO3在氢氧化钠碱性介质中发生歧化反应,其过渡态产物能与Luminol发生化学发光反应.维生素B1能使体系的发光强度减弱,化学发光强度的下降值(ΔI)与维生素B1的浓度在1.0×10-7～1.0×10-5mol·L-1范围内呈良好的线性关系,从而建立了测定维生素B1的流动注射化学发光新方法.方法的检出限为5.0×10-8mol·L-1.方法用于维生素B1片剂的测定.