Oxygen‐binding Protein Fiber and Microgel: Supramolecular Myoglobin–Poly(acrylate) Conjugates |
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| Authors: | Dr. Toshikazu Ono Yasushi Hisaoka Dr. Akira Onoda Dr. Koji Oohora Prof. Dr. Takashi Hayashi |
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| Affiliation: | 1. Department of Chemistry and Biochemistry, Center for Molecular Systems (CMS), Kyushu University, Fukuoka, Japan;2. Japan Science and Technology Agency (JST)-PRESTO, Saitama, Japan;3. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Japan;4. Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan;5. +81)?6‐6879‐7930 |
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| Abstract: | A supramolecular conjugate of myoglobin (Mb) and water‐soluble poly(acrylate), (PA5k and PA25k, where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme‐heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme‐propionates is coupled to the side‐chain carboxyl groups of poly(acrylate) activated by N‐hydroxysuccinimide. The ratios of the heme‐modified monomer unit and the unmodified monomer unit (m:n) in the polymer chains of Heme‐PA5k and Heme‐PA25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb‐connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme‐modified polymer and dimeric apomyoglobin as a cross‐linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity. |
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| Keywords: | biomaterials conjugation gels hemoprotein microgels protein– polymer hybrids |
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