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A recombinant human tumour necrosis factor (rhTNF) cDNA was constructed. The TNF gene was isolated from a human genomic gene library. There are four exons in the TNF gene. The fourth exou codes for 140 amino acids of the TNF matured protein which is composed of 157 amino acids. A major portion of the fourth exon was isolated and then ligated to a synthesized DNA fragment coding for the remaining amino acids. The partial synthetic hTNF (rhTNF) cDNA thus generated was subcloned into a vector and successfully expressed in E. coli. 5-1 fer1entator was used to produce rhTNF. About 20g (wet weight) of bacterial pellet per liter medium and 106—10~7 units of cytotoxicity to L929 cells per milliliter medium were obtained. rhTNF was purified by HPLC and dried with a freeze dryer, rhTNF with a purity of about 95% in the form of white powder was obtained. The sequence of ten amino acids at the amino terminus of the rhTNF was determined. The result showed that it was identical with that of the natural human TNF. 相似文献
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分别对番茄果肉及果芯的固形物进行低温分离提取,并分别采用这二种提取物对大鼠血栓形成的影响进行了研究.实验结果发现,这种提取物对大鼠的血栓形成有明显的抑制作用,其中,果芯固形物对大鼠血栓抑制效果优于果肉固形物. 相似文献
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食用天然色素密蒙黄稳定性分析研究 总被引:2,自引:0,他引:2
研究了食品中常见的几种金属离子Na^ 、Cu^2 、Al^3 、Fe^3 、Sn^2 和维生素C、苯甲酸等食品添加剂对食用天色色素密蒙黄稳定性的影响,并对色素的耐碱性和耐热性进行了探讨,结果表明其性能稳定。本研究为密蒙黄色素的开发应用提供了依据。 相似文献
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