A Method to Assess Radiochemical Purity of Compounds Measured by Radioimmunoassay

Abstract

A method is described for assessing radiochemical purity of compounds measured by radioimmunoassay. This method is applicable in cases when chromatography is used prior to the radioimmunoassay proper. In individual fractions of the chromatographic zone containing the compound assayed, both radioactivity (e. g. in DPM) and mass (e. g. in pg) are measured. The measurement of mass is performed using radioimmunoassay. Radiochemical purity is assumed, if the radioactivity/mass ratio (specific activity) in individual fractions of the zone is constant. The constancy of specific activity is tested statistically using a regression analysis. A radioimmunoassay of progesterone in a blood plasma pool collected during the follicular phase of the menstrual cycle was used to demonstrate the practical features of the assessment of radiochemical purity. Radiochemical pure progesterone could be obtained only after repeated chromatography. Purification to radiochemical purity resulted in a significantly decreased estimate of progesterone contents in the plasma pool investigated. A value of 344 pg/ml was obtained, in contrast to the value of 422 pg/ml found prior to the repeated chromatography. The former value can be considered an accurate and valid estimate.     

Monitoring the Dynamics of Phase Separation in a Polymer Blend by Confocal Imaging and Fluorescence Correlation Spectroscopy

The phase separation of the polymer blend polystyrene/poly(methyl phenyl siloxane) (PS/PMPS) is studied in situ by laser scanning confocal microscopy (LSCM) and by fluorescence correlation spectroscopy (FCS) at macroscopic and microscopic length scales, respectively. It is shown for the first time that FCS when combined with LSCM can provide independent information on the local concentration within the phase‐separated domains as well as the interfacial width.     

Application Of Fluorescamine Xiv1. Determination Of Optical Purity Of A Small Amount Of Amino Acids By The Fluorescamine Method

Abstract

A new simple and fast micro method has been established to determine the optical purity of a small amount of amino acids (0.1–1 μg/ml). The amino acids react readily with fluorescamine to form pyrrolinone-type chromophores, which show strong CD bands at 400–300 nm and much larger optical rotation than the free amino acids in the visible spectral range.     

Study on Quantum Entanglement Between Mesoscopic Circuit and Environment at Coherent State

Taking into account the interaction between electrons and phonons, in the case without-rotating-wave approximation, we study the entangling property between the mesoscopic circuit and environment at coherent state or equilibrium state. The result indicates that, in long time limit t→∞, the averages of charge and current in the circuit only depend on the average of the system at the initial state when the environment is initially at thermal equilibrium. However, when the environment is initially at coherent state, the average of charge and current in the circuit is determined by the specific coherent state ensemble. Generally speaking, the entanglement between the circuit and environment will lead to the quantum state purity declining of the circuit, then the circuit emerges decoherent phenomenon, and so a mixed state appears. Purity changes are related to the initial quantum state of environment and circuit. With the further evolution of time, coherence will be gradually restored, but cannot return to 1.     

高效液相色谱法测定非天然氨基酸的光学纯度

 以（1- 氟-2,4-二硝基苯基）-5-L-缬氨 酰胺为手性试剂、反相高效液相色谱法测定非天然氨基酸的光学 纯度。梯度洗脱,流动相A：含体积分数为0.1％的三氟乙酸的乙腈,流动相B：体积分 数为0.1％的三氟乙酸水溶液。L-和D-对映体得到良好分离。准确测定了25种非天然 氨基酸L-和D-对映体的光学纯度。     

A study on entanglement dynamics for a four-qubit model

In this paper, we consider the entanglement dynamics of a four-qubit model [2006 Phys. Rev. A 74 042328] where two entangled qubits a and b locally interact with separate qubits A and B via the spin-exchange-like Hamiltonian. We study the effect of purity of initial entangled state of qubits a, b on the entanglement evolution and its relation with energy transfer. Also, we find that the total bipartite entanglement of qubits a, b plus A, B is not a constant any longer when the initial entangled state of a, b is not pure, which is a complement to the result in the paper [2007 J. Phys. B 40 S45] for the pure case.     

高纯锗中浅受主的光热电离光谱

在液氦温度附近, 运用傅里叶变换光谱以及与之相连的磁光光谱系统, 对室温电阻率约为50Ω·cm的p型高纯锗样品进行了高灵敏度的光热电离光谱的研究.从实验上确定了高纯锗样品中浅杂质光热电离的最佳温度范围, 在该温度范围内测量了样品的光热电离光谱, 指出该样品中主要杂质为浅受主硼与铝. 对杂质谱线发生分裂的两种原因, 补偿性杂质导致的快速复合以及随机应力等, 进行了分析讨论.     

高纯硅中补偿性杂质的光热电离光谱

首先测量了高纯n型硅样品在接近液氦温度区域内随温度变化的光热电离光谱，确定了硅样品的最佳光热电离温度范围. 在该温度范围内，在有本征带隙光照射条件下，测量了样品的高分辨率光热电离光谱，同时观察到了来自主要浅杂质施主磷以及补偿性杂质硼的正信号. 随后，应用外加磁场，对硼的光热电离光谱进行了研究，发现来自硼的光热电离信号，在外加磁场作用下，发生了由正向负信号的转变. 通过对该现象进行分析讨论，排除了该现象是温度效应的可能，指出普遍用来解释补偿性杂质光热电离响应的Darken模型存在不足，而少数载流子快速复合模     

化学计量学用于决明子色谱指纹图谱峰纯度的鉴别

应用化学计量学方法鉴别色谱指纹图谱的峰纯度。对背景进行扣除后,用对照组分光谱构建正交投影矩阵对目标色谱峰的光谱进行投影,以目标色谱峰投影后的残余光谱与投影前的原始光谱的夹角余弦为判据鉴别目标色谱峰的峰纯度：用该方法对决明子药材色谱指纹图谱的峰纯度进行识别,鉴别出大黄素、大黄酚和大黄素甲醚三个纯色谱峰。此方法用于色谱指纹图谱峰纯度的鉴别,结果可靠。     

差示扫描量热法对芝麻酚纯度标准物质的定值

建立了采用差示扫描量热法对芝麻酚纯度标准物质的定值及不确定度评价的数学模型、有效检测技术和分析方法.采用差示扫描量热法测量芝麻酚样品纯度的实验条件为升温速率3.0 K/min,称样量3.4～4.7 mg,炉内气体为静态空气.对通过均匀性检验和长期稳定性考察的芝麻酚纯度标准物质进行定值和不确定度评价,同时采用高效液相色谱...