超声波声孔效应中气泡动力学的研究

在超声快速制取组织细胞病理切片的过程中，发现激励信号对切片制取效果有明显的影响.为了掌握超声激励信号对组织细胞的影响规律，达到快速制取病理切片的最佳状态，从气泡空化模型入手，通过改变激励信号频率、声压、气泡初始半径和液体黏滞系数等参量，研究了声孔效应中气泡动力学激励机制.数值计算表明：空化泡振动随激励声压增强而升高，随液体黏滞系数增强而减弱；一定频率范围内空化泡振动能保持在膨胀、收缩和振荡的稳定空化状态，存在空化泡稳态振动的最佳激励频率；一定初始半径能保证空化泡产生稳定的振动，存在空化泡稳态振动幅度最大的初始半径.实际操作中，在频率、声压、初始半径和黏滞系数综合作用的若干空化阈内，声孔效应使超声快速法制取细胞组织切片获得最佳效果. 关键词： 声孔效应 超声空化 气泡振动 稳态空化域     

热声致冷效应演示实验

基于热声致冷原理,利用自制的热声堆,采用常见的材料和仪器,设计了扬声器驱动热声致冷实验演示装置.以空气作工质,在无冷却措施的情况下,系统运行200 s后,实现了13℃的降温及25℃的温跨.     

摆动式声多普勒效应实验仪

针对现有的声多普勒效应实验装置中存在的问题,设计并制作了摆动式声多普勒效应实验仪.该仪器能够分别进行声源运动和观察者运动的声多普勒效应实验,且可定量分析频偏和测量速度.本文详细介绍了该仪器的原理、构造、特点和实验过程,并对实验结果进行了较为全面的分析.     

回热器的热声直流模型及其效应研究

本文由基本的守恒方程出发,获得了能描述回热器存在声直流时的非线性动力学模型及由此而导致的非线性时均热力学效应。根据所得到的模型,考察了声直流对临界声功产生率温度梯度、回热器温度分布以及脉冲管制冷性能的影响。     

拓扑声子与声子霍尔效应

拓扑学与物理的结合是近几十年物理学蓬勃发展的一个新领域,它不仅活跃在量子场理论以及高能物理中,更广泛地存在于凝聚态物理体系中,包括量子(反常、自旋)霍尔效应和拓扑绝缘体(超导体)等.声子是凝聚态体系中热输运的主要载体;最近由于各种声子器件的发现,声子学得到了广泛的关注.本文介绍了声子的拓扑性质以及声子的霍尔效应现象,分别评述了在破坏时间反演对称、破坏空间反演对称、以及同时破坏时间和空间反演对称三种情况下所产生的声子霍尔效应、声子谷霍尔效应等相关物理研究进展.最后对拓扑学在其他声学体系中的应用做了简单介绍,并进一步讨论了其未来的发展方向.     

利用光声效应研究ITO薄膜的光吸收率

利用基于压电效应的光声技术,研究了ITO薄膜在可见光波段的吸收特性,并与分光型光学薄膜分析系统NKD8000测得的数据进行了比较,得到了一致性较好的结果。实验证实:在可见光波段,该ITO薄膜的吸收率随波长呈非线性变化,在450nm附近吸收最强,随着波长的增加逐渐减小,在514.7nm处接近于零,之后又缓慢增大。     

二维声电光效应的耦合波方程理论

按照参量互作用的观点二维电光效应的耦合波方程,并给出二维怕电光效应的衍射效率计算公式。     

多维正常声电光效应最佳工作模式的确定

通过对四方、立方晶系的纵向电光效应和多维正常声光效应的研究，选定了四方晶系的KDP晶体和立方晶系的GaP晶体作为多维正常声电光器件的最佳晶体，并通过计算两种晶体的声光互作用性能参量，确定了它们的二维、三维、四维正常声电光效应的最佳工作模式。研究结果为多维正常声电光器件的设计与制作打下了良好基础。多维声电光器件在多路光通信和多通道光信息处理中有应用前景。     

固体中光声喇曼效应的原理特性分析

从相干喇曼放大过程出发,运用准平衡模型以及热弹理论,对固体样品中光声喇曼效应进行理论分析,导出了脉冲激光泵浦下的光声喇曼信号表达式,并做了数值估算.总结分析了固体介质中光声喇曼效应的一些特性.     

热声交变流动中浮力效应理论分析

热声交变流动是热声热机的工作基础,研究交变流动机理有助于人们认识热声热机的工作过程。鉴于前人的理论分析一般没有考虑浮力效应的影响,本文从流体热动力学的角度研究了浮力对流与热声交变流动的耦合规律。基于线性理论方法,给出了考虑浮力项和热对流Rayleigh数的热声线性理论模型,分析了浮力效应对平行交变流动的影响,发现热对流...     

Microstreaming velocity field and shear stress created by an oscillating encapsulated microbubble near a cell membrane

Sonoporation mediated by microbubbles is being extensively studied as a promising technology to facilitate gene/drug delivery to cells. However, the theoretical study regarding the mechanisms involved in sonoporation is still in its infancy. Microstreaming generated by pulsating microbubble near the cell membrane is regarded as one of the most important mechanisms in the sonoporation process. Here, based on an encapsulated microbubble dynamic model with considering nonlinear rheological effects of both shell elasticity and viscosity, the microstreaming velocity field and shear stress generated by an oscillating microbubble near the cell membrane are theoretically simulated. Some factors that might affect the behaviors of microstreaming are thoroughly investigated, including the distance between the bubble center and cell membrane (d), shell elasticity (χ), and shell viscosity (κ). The results show that (i) the presence of cell membrane can result in asymmetric microstreaming velocity field, while the constrained effect of the membrane wall decays with increasing the bubble-cell distance; (ii) the bubble resonance frequency increases with the increase in d and χ, and the decrease in κ, although it is more dominated by the variation of shell elasticity; and (iii) the maximal microstreaming shear stress on the cell membrane increases rapidly with reducing the d, χ, and κ. The results suggest that microbubbles with softer and less viscous shell materials might be preferred to achieve more efficient sonoporation outcomes, and it is better to have bubbles located in the immediate vicinity of the cell membrane.     

Regulation of shear stress on rolling behaviors of HL-60 cells on P-selectin

Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1(PSGL-1), and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm2. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing fractional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mechanism for most cell rolling events through P-selectin.     

微剪切应力传感器的加工工艺

提出了一种感测单元不直接接触流场的微剪切应力传感器结构,详细阐述了其感测单元MEMS制作工艺。采用热氧化硅掩膜方法解决了硅深刻蚀的选择比问题;优化后的硅深刻蚀工艺参数：刻蚀功率1600 W、低频(LF)功率100 W,SF6流量360 cm3/min,C4F8流量300 cm3/min,O2流量300 cm3/min。采用Cr/Au掩膜,30 ℃恒温低浓度HF溶液解决了玻璃浅槽腐蚀深度控制问题;喷淋腐蚀和基片旋转等措施提高了玻璃浅槽腐蚀表面质量。采用上述MEMS工艺制作了微剪切应力传感器样品,样品测试结果表明：弹性悬梁长度和宽度误差均在2 m以内、玻璃浅槽深度误差在0.03 m以内、静态电容误差在0.2 pF以内,满足了设计要求。     

Effects of residual stress and interfacial frictional shear stress on interfacial debonding at notch-tip in two-dimensional unidirectional composite

A calculation method based on the shear lag approach was presented to get an approximate estimate of influences of residual stresses and frictional shear stress at the debonded interface on the interfacial debonding behavior at the notch-tip along fiber direction in two-dimensional unidirectional double-edge-notched composites. With this method, the energy release rate for initiation and growth of debonding as a function of composite stress were calculated for some examples. The calculation results showed in outline how much the tensile and compressive residual stresses in the matrix and fiber along fiber direction, respectively, act to hasten the initiation and growth of the debonding when the final cut element in the notch is matrix, while they act to retard them when the final cut element is fiber, and how much the frictional shear stress at the debonded interface reduces the growth rate of the debonding.     

Micro thermal shear stress sensor based on vacuum anodic bonding and bulk-micromachining

This paper describes a micro thermal shear stress sensor with a cavity underneath, based on vacuum anodic bonding and bulk micromachined technology. A Ti/Pt alloy strip, 2μm×100μm, is deposited on the top of a thin silicon nitride diaphragm and functioned as the thermal sensor element. By using vacuum anodic bonding and bulk-si anisotropic wet etching process instead of the sacrificial-layer technique, a cavity, functioned as the adiabatic vacuum chamber, 200μm×200μm×400μm, is placed between the silicon nitride diaphragm and glass （Corning 7740）. This method totally avoid adhesion problem which is a major issue of the sacrificial-layer technique.     

基于剪应力检测的石英微结构及其陀螺效应研究

为了简化石英陀螺结构侧壁上的电极图形,设计了一种基于剪应力检测Coriolis力的新型石英微结构.通过对剪应力检测方案的分析,微结构侧壁电极的极性单一,采用锥度为15°的锥形梁结构作为敏感梁的设计可以增大敏感梁的检测灵敏度.通过各项异性湿法腐蚀z切石英片的方法制造了微结构样件.测试表明样件的驱动频率约为5.6kHz,大气下的品质因子在5000以上.输出信号频谱的谱线存在Coriolis力信号,验证了设计思想的正确性。     

Finite-element analysis on performance and shear stress of cemented tungsten carbide anvils used in the China-type cubic-anvil high-pressure apparatus

Stress distribution in cemented tungsten carbide (WC) anvils is of primary interest in high pressure research, but is very difficult to determine experimentally. We have performed finite-element simulations to study the performance and the shear stress distribution of WC anvils used in the China-type cubic-anvil high pressure apparatus (SPD-6×2000). Our results show that in order to avoid failure cracks in high-stress areas, the maximum shear stress should be lower than 3.17 GPa for the WC anvils (8% cobalt). The simulations have been verified by high-pressure experiments. Our method of analyzing stress distribution helps in evaluating the ability of WC anvils, and would aid in designing a new anvil to enhance performance.     

Co-registration of the distribution of wall shear stress and 140 complex plaques of the aorta

Previous studies provide evidence that atherosclerosis develops in vascular regions exposed to low wall shear stress (WSS) and high oscillatory shear index (OSI). 4D flow MRI was analyzed in 70 stroke patients with complex plaques (≥ 4 mm thickness, ulcerated or superimposed thrombi) and in 12 young healthy volunteers. The segmental distribution of peak systolic WSS_systole and OSI was quantified in analysis planes positioned directly at the location of 140 complex plaques found in the 70 patients. In addition, WSS_systole and OSI were evaluated in 8 standard analysis planes distributed along the aorta. Complex plaques were predominantly found at the inner curvature of the aortic arch and of the descending aorta. High OSI was co-located with the segments mostly affected by complex plaque while WSS_systole demonstrated a homogenous distribution. In standard analysis planes, patients demonstrated significantly (p < 0.01) altered distribution of wall parameters compared to volunteers (reduced WSS_systole in 91% of aortic wall segments, increased OSI in 48% of segments). OSI distribution was asymmetric with higher values at the inner curvature of the aorta. While WSS and OSI showed expected changes in patients compared to healthy controls, their distribution pattern at complex plaques indicated a more complex and heterogeneous relationship than previously anticipated.     

Monitoring the effect of sonoporation on the cells using electrochemical approach

Sonoporation is applied to enhance the permeability of the cell to bioactive materials by employing the acoustic cavitation of microbubbles. This phenomena would be helpful in molecular biology, delivery of large molecules into the cells and gene therapy. Many methods have been applied to monitor the biological effects and trace of sonoporation on the cells such as scanning/transmission electron microscopy, confocal imaging and flow cytometry. Here, we monitored the effect of sonoporation on the cells using electrochemical method with an integrated three electrode system. Electrochemical responses of stimulated cells, compared to flow cytometry and electron microscopy results, presented different patterns of sonoporation in the cells detectable by cyclic voltammetry. In addition, confocal microscopy from actin stress fibers and young’s modulus measured by AFM revealed the correlation of cell mechanics and amount of induced sonopores in the cells. This method could be applied as a new trend in cellular mechanochemical studies.