| PCR检测伪狂犬病病毒的研究 |
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| 引用本文： | 王家富,丁建华,张光道,罗满林,张楚瑜.PCR检测伪狂犬病病毒的研究[J].武汉大学学报(理学版),1996(6). |
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| 作者姓名： | 王家富 丁建华 张光道 罗满林 张楚瑜 |
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| 作者单位： | 武汉大学生命科学学院，同济医科大学 |
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| 摘 要： | 用ＰＣＲ的方法，扩增了伪狂犬病病毒（ＰＲＶ）ｇｐ５０基因的一段较为保守区（２１６ｂｐ）．检测了不同ＰＲＶ毒株感染的ＢＨＫ细胞以及接种的家兔，并对ＰＣＲ检测的灵敏性作了初步研究，结果表明，ＰＣＲ法扩增ｇｐ５０基因具有较高特异性和灵敏性，是检测ＰＲＶ的有效方法
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| 关 键 词： | 伪狂犬病病毒，PCR，基因检测 |
STUDIES ON PCR FOR DETECTION OF PSEUDORABIES VIRUS |
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| Wang Jiafu,Ding Jianhua,Zhang Guangdao,Luo Manling,Zhang Chuyu.STUDIES ON PCR FOR DETECTION OF PSEUDORABIES VIRUS[J].JOurnal of Wuhan University:Natural Science Edition,1996(6). |
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| Authors: | Wang Jiafu Ding Jianhua Zhang Guangdao Luo Manling Zhang Chuyu |
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| Abstract: | In this paper,a conservative fragment(216 bp) in gp50 gene of pseudorabies virus(PRV) was amplified by PCR technique.BHK 21 cells infected by different kinds of PRV strains and inoculated rabbits were detected.The sensitivity of PCR detection was also studied.The results demonstrated that PCR technique was quite special and sensitive to amplify gp50 gene and it was an effective method for detection PRV. |
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| Keywords: | Pseudorabies virus(PRV) PCR Gene detection |
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