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| 水解酶在二乙基锌与芳香醛加成反应中的应用(英文) | |
| 引用本文： | 魏晓飞,郑清川,吉腾飞,赵博,李春元,王萍,曹淑桂,王智,王磊.水解酶在二乙基锌与芳香醛加成反应中的应用(英文)[J].催化学报,2009,30(5). |
| 作者姓名： | 魏晓飞 郑清川 吉腾飞 赵博 李春元 王萍 曹淑桂 王智 王磊 |
| 作者单位： | 1. 吉林大学分子酶学工程教育部重点实验室,吉林长春,130023 2. 吉林大学理论化学计算国家重点实验室,吉林长春,130023 3. 中国医学科学院药物研究所,北京,100050 |
| 基金项目： | 国家自然科学基金，Science Technology Development Program of Jilin Province for Youths |
| 摘 要： | 报道了水解酶催化二乙基锌与芳香醛的加成反应,对反应条件进行了优化.在最适条件(嗜热酯酶APE1547为酶源,反应温度40℃,氯仿为溶剂,4-Cl-苯甲醛为底物)下,加成反应生成的光学活性醇产率最高达78%,ee值最高可达56%.通过实验结果和分子动力学分析对可能的反应机理进行了推测.本研究进一步拓展了酶的非专一性. |
| 关 键 词： | 水解酶 二乙基锌 芳香醛 加成 酶 非专一性 |
Addition of Diethylzinc to Aromatic Aldehydes Catalyzed by Hydrolase |
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| WEI Xiaofei,ZHENG Qingchuan,JI Tengfei,ZHAO Bo,LI Chunyuan,WANG Ping,CAO Shugui,WANG Zhi,WANG Lei.Addition of Diethylzinc to Aromatic Aldehydes Catalyzed by Hydrolase[J].Chinese Journal of Catalysis,2009,30(5). | |
| Authors: | WEI Xiaofei ZHENG Qingchuan JI Tengfei ZHAO Bo LI Chunyuan WANG Ping CAO Shugui WANG Zhi WANG Lei |
| Institution: | WEI Xiaofei1,ZHENG Qingchuan2,JI Tengfei3,ZHAO Bo1,LI Chunyuan1,WANG Ping1,CAO Shugui1,WANG Zhi1,WANG Lei1,1Key Laboratory of Molecular Enzymology , Engineering of Ministry of Education,Jilin University,Changchun 130023,Jilin,China 2State Key Laboratory of Theoretical , Computational Chemistry,China 3Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing 100050,China |
| Abstract: | A novel application of hydrolase as catalyst for the addition of diethylzinc to aromatic aldehydes is reported.Optimum conditions were screened out.Under the optimum conditions(i.e.,reaction at 40 oC using hyperthermophilice esterase APE1547,4-Cl-benzaldehyde substrate,and trichloromethane solvent),this reaction provided optically active secondary alcohol with an enantiomeric excess up to 56% and a yield up to 78%.The possible mechanism was proposed based on experimental observations and molecular dynamics ... |
| Keywords: | hydrolase diethylzinc aromatic aldehyde addition enzyme promiscuity |
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