纳米-微米复合孔泡沫陶瓷固定化脂肪酶

考察了泡沫陶瓷的孔径分布和表面性质对脂肪酶固定化的影响.研究表明,泡沫陶瓷的纳米孔孔径分布非常适合脂肪酶的固定化,对固定化酶的催化效率有决定性的影响.经1h的定化,泡沫陶瓷固定化酶的活性达商业化硅藻土固定化酶的1.33倍,体积活力为其2.63倍,蛋白载量为45.36mg/g陶瓷,比活为1215.39U/g,活力回收为41.2%.泡沫陶瓷固定化脂肪酶在有机相乙酸乙酯合成中表现优良,连续使用5次,每次反应3h,乙酸转化率均在93%左右.

Immobilization of Lipase on Nano- and Microporous Ceramic Foam

The effect of pore size distribution and superficial properties of the ceramic foam was investigated. The nanopore size distribution of the ceramic foam was very suitable to immobilize lipase and had crucial effect on the catalytic efficiency of the immobilized lipase. After 1 h immobilization, the lipase activity per gram carrier on the ceramic foam was 1.33 times that on commercial diatomite, and the volume activity was 2.63 times. The protein loading was 45.36 mg/g, specific activity 1 215.39 U/g, and activity recovery 41.2%. The batch operation stability of the immobilized lipase on the ceramic foam was good in the synthesis of ethyl acetate in an organic phase. The acetic acid conversion in the 5-batch continual operation was maintained at nearly 93% after 3 h reaction in each batch.