Probing the Adhesion of Hepatocellular Carcinoma HepG2 and SK‐Hep‐1 Cells |
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| Authors: | Shuchen Hsieh Pei‐Ying Lin Chiung‐Wen Hsieh I‐Tin Li Shu‐Ling Hsieh Chih‐Chung Wu Yun‐Shan Huang Huay‐Min Wang Li‐Wei Tu Kuang‐Hung Cheng Hay‐Yan Jack Wang Deng‐Chyang Wu |
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| Institution: | 1. Department of Chemistry, National Sun Yat‐sen University, Kaohsiung, 80424, Taiwan;2. Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 81157, Taiwan;3. Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan 71101, Taiwan;4. Department of Food Science and Technology, Tajen University, Pingtung, 90741, Taiwan;5. Kaohsiung Veterans General Hospital, 386 Ta‐Chung 1st Rd, Kaohsiung 81362, Taiwan;6. Department of Physics, National Sun Yat‐sen University, Kaohsiung 80424, Taiwan;7. Institute of Biomedical Sciences, National Sun Yat‐sen University, Kaohsiung 80424, Taiwan;8. Department of Biological Sciences, National Sun Yat‐sen University, Kaohsiung 80424, Taiwan;9. Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan |
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| Abstract: | Carcinoma cell differentiation stage is an important indicator of cell behavior. For example, cell mobility is much higher for poorly‐differentiated hepatocellular carcinoma SK‐Hep‐1 cells than for well‐differentiated HepG2 cells. In this study, we have cultured HepG2 and SK‐Hep‐1 cells on chemically patterned polydimethylsiloxane (PDMS) substrates to observe differences in the adhesion properties and cell structure that occur due to the patterns. Both cell lines showed a preference for the hydrophobic regions on the patterned PDMS surface with SK‐Hep‐1 cells achieving a higher density than HepG2 for the same cell‐count solutions. Further, SK‐Hep‐1 cells adopted the square or hexagonal shape of the surface patterns while HepG2 cells maintained their more rounded shape. AFM force measurement arrays were also performed on the cell surfaces to measure and map adhesion values between the tip and cell surface membrane. These results demonstrate that, in addition to cell shape and size, adhesion expression in hepatocellular carcinoma cells is differentiation stage dependent. Further, the ability of the SK‐Hep‐1 cells to adopt the shape of the substrate pattern indicates they are more structurally labile, which may contribute to their higher mobility. |
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| Keywords: | Hepatocellular carcinoma cells AFM PDMS |
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