Determination of limonin in dog plasma by liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
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Authors: | Shi‐jia Liu Ling Zhou Jun Zhang Chang‐yin Li Zi‐xiu Liu Wen‐zheng Ju |
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Institution: | 1. Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, , Nanjing, 210029 China;2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, , Nan Jing, 210009 Jiangsu, China;3. Nanjing University of Traditional Chinese Medicine, , Nanjing, 210029 Jiangsu, China |
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Abstract: | A highly sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for the determination of limonin in beagle dog plasma using nimodipine as internal standard. The analyte and internal standard (IS) were extracted with ether followed by a rapid isocratic elution with 10 mm ammonium acetate buffer–methanol (26:74, v/v) on a C18 column (150 × 2.1 mm i.d.) and subsequent analysis by mass spectrometry in the multiple reaction monitoring mode. The precursor to product ion transitions of m/z 469.4 → 229.3 and m/z 417.2 → 122.0 were used to measure the analyte and the IS. The assay was linear over the concentration range of 0.625–100 ng/mL for limonin in dog plasma. The lower limit of quantification was 0.312 ng/mL and the extraction recovery was >90.4% for limonin. The inter‐ and intra‐day precision of the method at three concentrations was less than 9.9%. The method was successfully applied to pharmacokinetic study of limonin in dogs. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | limonin LC– MS/MS electrospray ionization dog plasma pharmacokinetics |
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