| Refolding of Denatured／Reduced Lysozyme Using Weak－Cation Exchange Chromatography |
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| 作者姓名： | YanWANG BoLinGONG XinDuGENG |
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| 作者单位： | InstituteofModemSeparationScience,KeyLaboratoryofModemSeparationScienceinShaanxiProvince,NorthwestUniversity,Xi‘an710069 |
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| 摘 要： | Oxidative refolding of the denatured/reduced lysozyme was investigated by using weak-cation exchange chromatography (WCX). The stationary phase of WCX binds to the reduced lysozyme and prevented it from forming intermolecular aggregates. At the same time urea and ammonium sulfate were added to the mobile phase to increase the elution strength for lysozyme. Ammonium sulfate can more stabilize the native protein than a common eluting agent,sodium chloride. Refolding of lysozyme by using this WCX is successfully. It was simply carried out to obtain a completely and correctly refolding of the denatured lysozyme at high concentration of 20.0 mg/mL.
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| 关 键 词： | 变性 溶解酵素 弱阳离子交换色谱分析 蛋白质 生物化学 |
Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography |
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| YanWANG BoLinGONG XinDuGENG.Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography[J].Chinese Chemical Letters,2003,14(8):828-831. |
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| Authors: | Yan WANG Bo Lin GONG Xin Du GENG |
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| Abstract: | Oxidative refolding of the denatured/reduced lysozyme was investigated by using weak-cation exchange chromatography (WCX). The stationary phase of WCX binds to the reduced lysozyme and prevented it from forming intermolecular aggregates. At the same time urea and ammonium sulfate were added to the mobile phase to increase the elution strength for lysozyme. Ammonium sulfate can more stabilize the native protein than a common eluting agent, sodium chloride. Refolding of lysozyme by using this WCX is successfully. It was simply carried out to obtain a completely and correctly refolding of the denatured lysozyme at high concentration of 20.0 mg/mL. |
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| Keywords: | Weak-cation exchange chromatography lysozyme protein refolding |
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