动态超声萃取分光光度法在线测定红花中的总红花黄色素

用动态超声萃取分光光度法在线测定了红花中的红花黄色素, 对萃取条件进行了优化. 萃取与测定同时进行, 大大缩短了样品的分析时间, 简化了分析过程. 通过与静态超声萃取、振荡萃取和索式萃取进行比较, 不仅证明该方法耗时最短, 而且样品用量小, 萃取产率较高. 实际样品分析得到了满意的结果.

Determination of Total Safflower Yellow Pigments in Carthamus tinctorius L. by Dynamic Ultrasonic Extraction Coupled with On-line Spectrophotometric Detection

A dynamic ultrasonic extraction(DUE)coupled with on-line detection by spectrophotometry was proposed for the determination of total safflower yellow pigments(calculated against safflor yellow A)in Carthamus tinctorius L.The extraction was performed in a common self-made extraction vessel placed inside an ultrasonic bath and a peristaltic pump was used to deliver the solvent.Several parameters of DUE,including flow rate of extraction solvent,ultrasonic power and sample amount,were optimized.Compared to the off-line method,the on-line approach has the advantages of minimum sample amount(5 mg),on-line monitoring the extraction process and time-saving(10 min)and the method is more convenient for rapid optimization of the extraction process.