白藜芦醇的电化学行为及其与DNA的相互作用

采用电化学方法研究了白藜芦醇在pH=2~13的缓冲溶液中的电化学行为、抗氧化能力及其与DNA的相互作用. 研究结果表明， 在2.011的溶液中, 白藜芦醇产生的P4和P5波分别是其二价和三价阴离子的还原波. 在最佳实验条件下, 微分脉冲极谱图上IP3在8.0×10-8~2.0×10-6 mol/L范围内与白藜芦醇的浓度呈线性关系, 检出限为4×10-8 mol/L. 将该法用于中药虎杖中白藜芦醇含量的测定, 结果与高效液相色谱法一致.

Electrochemical Behavior of Resveratrol and Its Interaction with DNA

Electrochemical techniques were employed to study the electrochemical behavior in a wider pH range, the antioxidative ability and the interaction with DNA of resveratrol. The experimental results show that wave P1 detected in 2.011. Under the optimum conditions, a linear relationship could be established between the peak current of wave P3 and the concentration of resveratrol in the range from 8.0×10-8 to 2.0×10-6 mol/L, and the detection limit was 4×10-8 mol/L. The determination result of content of resveratrol in Chinese traditional herbal medicine Polygonum cuspidatum by the proposed differential pulse polarography method was consistent with the result by the comparison HPLC method. The antioxidative ability of resveratrol and the interaction mode of resveratrol with DNA were proved by electrochemical method.