| 功能性CdS纳米荧光探针荧光增敏法测定人血清白蛋白 |
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| 引用本文： | 汪乐余,周运友,朱昌青,张明翠,陶海升,王伦.功能性CdS纳米荧光探针荧光增敏法测定人血清白蛋白[J].高等学校化学学报,2003,24(4):612-614. |
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| 作者姓名： | 汪乐余 周运友 朱昌青 张明翠 陶海升 王伦 |
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| 作者单位： | 安徽师范大学化学与材料科学学院, 芜湖 241000 |
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| 基金项目： | 国家教育部高等学校骨干教师资助项目 (批准号 :99JL 0 79)资助 |
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| 摘 要： | 目前 ,以荧光分析法对蛋白质进行研究主要采用有机荧光探针1～ 3 ] .与传统的有机染料 (如罗丹明 )探针相比 ,半导体纳米晶体探针的光强度要高 2 0倍 ,光稳定性要高 1 0 0倍 ,谱线宽度只是有机染料谱线宽度的 1 /3 4 ] .将半导体纳米晶体作为探针用于测定生物分子 ,将大大提高分析的灵敏度和选择性 ,而目前其应用于生物染色、医疗诊断、DNA序列测定和免疫分析等方面的研究很少 4 ,5] .本文合成了胶态纳米粒子 Cd S,并在其外表面修饰一层巯基乙酸 ,使其具有水溶性 ,并能与生物分子作用 ,从而可利用其外表面的功能性基团对人血清白蛋白进…
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| 关 键 词： | CdS纳米粒子 荧光探针 人血清白蛋白 |
| 文章编号： | 0251-0790(2003)04-0612-03 |
| 收稿时间： | 2001-11-19 |
Fluorescence Enhancing Method for the Determination of Human Serum Albumin with Functionalized CdS Nanoparticles as Fluorescence Probes |
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| WANG Le Yu,ZHOU Yun You,ZHU Chang Qing,ZHANG Ming Cui,TAO Hai Sheng,WANG Lun.Fluorescence Enhancing Method for the Determination of Human Serum Albumin with Functionalized CdS Nanoparticles as Fluorescence Probes[J].Chemical Research In Chinese Universities,2003,24(4):612-614. |
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| Authors: | WANG Le Yu ZHOU Yun You ZHU Chang Qing ZHANG Ming Cui TAO Hai Sheng WANG Lun |
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| Institution: | College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China |
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| Abstract: | CdS nanoparticles were prepared and modified with mercaptoacetic acid. The functionalized nanoparticles were water soluble and biocompatible and used as fluorescence probe in the determination of Human serum albumin(HSA) and γ globulin(γ G). Under the optimal conditions, linear relationships were found between the enhanced intensity of fluorescence and the concentration of protein in the range of 0.2 and 3.5 μg/mL for HSA and 0.2 and 11.0 μg/mL for γ G. The limits of detection were 0.09 μg/mL for HSA and 0.18 μg/mL for γ G. |
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| Keywords: | CdS Nanoparticle Fluorescence probe Human serum albumin |
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