| DNA电化学传感器在DNA损伤研究中的应用 |
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| 引用本文： | 孙星炎,徐春,刘盛辉,何品刚,方禹之.DNA电化学传感器在DNA损伤研究中的应用[J].高等学校化学学报,1998,19(9):1393-1396. |
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| 作者姓名： | 孙星炎 徐春 刘盛辉 何品刚 方禹之 |
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| 作者单位： | 华东师范大学化学系, 上海, 200062 |
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| 摘 要： | 利用单链DNA分子共价固定在石墨电极表面,采用核酸分子杂交技术,以道诺霉素作为杂交指示剂形成DNA电化学传感器.研究了在不同致突变剂的作用下,特定碱基序列的DNA在电极表面能否杂交及杂交程度的差异,探讨了DNA突变情况及可能的突变机理.
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| 关 键 词： | DNA 电化学传感器 道诺霉素 核酸分子杂交 伏安法 |
| 收稿时间： | 1997-12-01 |
Application in the Damage of DNA by Electrochemical DNA Sensor |
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| SUN Xing-Yan,XU Chun,LIU Sheng-Hui,HE Pin-Gang,FANG Yu-Zhi.Application in the Damage of DNA by Electrochemical DNA Sensor[J].Chemical Research In Chinese Universities,1998,19(9):1393-1396. |
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| Authors: | SUN Xing-Yan XU Chun LIU Sheng-Hui HE Pin-Gang FANG Yu-Zhi |
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| Institution: | Department of Chemistry, East China Normal University, Shanghai, 200062 |
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| Abstract: | Mutation and damage in DNAby interaction, UV-irralation, nitrite action and ultrasonication were studied by means of voltammetry using a graphite electrode covalently immobilied with ssDNA-1. Daunomycin was used as a hybidization indicator. When the complementary ssDNA-2 in solution was damaged by phsical or chemical agents, the hybridization of ssDNA-1 and ssDNA-2 was changed. All the results in voltammogram indicated that intercalator can't destroy the double-helical structure of DNA, while UV-irralation and nitrite action can make the permanent damage. Ultrasonication can reduce polymerization in DNAand the hybridization time was shortened. This may provide a new method for clinical medicine study. |
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| Keywords: | DNA Electrochemical sensor Daunomycin Hybridization of nucleic acid molecules Voltammetry |
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