离子液体均匀提取-高效液相色谱法测定参附注射液中4种单酯型乌头碱的含量

摘要 采用离子液体均匀提取-高效液相色谱法测定了参附注射液中苯甲酰乌头碱（BA）、苯甲酰新乌头碱（BM）、苯甲酰次乌头碱（BH）和苯甲酰脱氧乌头碱（BD）的含量，通过优化离子液体的用量，样品溶液的pH值，提取时间和盐浓度等参数，确定最佳提取条件为：pH为中性，盐浓度为5%，采用0.058g的C6Mim] BF4]和0.26g的 NH4PF6作为提取剂，提取1min，可有效地将目标物从5mL注射液中提取出来。在此实验条件下测得四种单酯型乌头碱的检测限分别为45.5（BM）,19.8(BA)，26.3(BH)和12.2(BD)μg L-1，对参附注射液样品进行分析，加样回收率范围在93.74-107.91%，RSD值均小于4.34%。

Determination of Four Kinds of Monoester-diterpenoid Aconines in SHEN-FU Injection by Homogeneous Ionic Liquid Microextraction-High Performance Liquid Chromatography

Abstract The determination of benzoylaconine(BA), benzoylmesaconine(BM), benzoylhypaconine(BH) and benzoyldeoxyaconine(BD) in SHEN-FU injection baced on homogeneous lonic liquid microextraction coupled with high-performance liquid chromatographic separation was described. The experimental parameters including volume of ionic liquid, pH, extraction time and salt concentration were optimizated, when 0.058g C6Mim] BF4] and 0.26g NH4PF6 were used as extraction solvent, the target compounds can be extracted from 5mL of injection, the extraction time is 1 min,neutral and the concentration of NaCl is 5%.The detection limits for BA,BM,BH and BD were 45.5,19.8,26.3 and 12.2,respectively. The recoveries of the analytes ranged from 93.74 to 107.91% and relative standard deviations were lower than 4.34%.