| 新型毛细管等电聚焦驱动方法的建立及其应用 |
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| 引用本文： | 朱贵杰,杨春,刘和春,张丽华,张维冰,张玉奎.新型毛细管等电聚焦驱动方法的建立及其应用[J].高等学校化学学报,2005,26(4):654-656. |
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| 作者姓名： | 朱贵杰 杨春 刘和春 张丽华 张维冰 张玉奎 |
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| 作者单位： | 中国科学院大连化学物理研究所, 国家色谱研究分析中心 大连 116011 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 毛细管等电聚焦(CIEF)在生物分离分析领域中的发展迅速．CIEF可分为两步法聚焦和一步法聚焦．电渗流(EOF)可作为一种新的驱动力．Rassi等根据串连体系中电渗流速率将被平均化的原理，将未涂层毛细管与聚乙二醇涂层的毛细管通过聚四氟乙烯管耦合起来，成功地利用毛细管区带电泳快速分离蛋白．此外，利用电渗流输液原理设计的电渗泵还可用作流动注射和微柱液相色谱的驱动系统。
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| 关 键 词： | 毛细管等电聚焦 电渗流驱动 蛋白质 多肽 |
| 文章编号： | 0251-0790(2005)04-0654-03 |
| 收稿时间： | 2004-10-27 |
Construction of a New Method for Mobilizing the Zones of CIEF and Its Application |
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| ZHU Gui-Jie,YANG Chun,LIU He-Chun,ZHANG Li-Hua,ZHANG Wei-Bing,ZHANG Yu-Kui.Construction of a New Method for Mobilizing the Zones of CIEF and Its Application[J].Chemical Research In Chinese Universities,2005,26(4):654-656. |
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| Authors: | ZHU Gui-Jie YANG Chun LIU He-Chun ZHANG Li-Hua ZHANG Wei-Bing ZHANG Yu-Kui |
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| Institution: | National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Science, Dalian 116011, China |
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| Abstract: | A simple method for mobilizing the focused zones of capillary isoelectric focusing(CIEF) for single-point detectors was developed, in which a linear polyacrylamide(LPA) coated capillary was connected with an uncoated capillary by an etched porous joint. The etched section allows electrical conductivity but blocks the hydrodynamic flow. The focused zones of mixed proteins was mobilized by this method successfully. Compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency. |
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| Keywords: | Capillary isoelectric focusing(CIEF) Electroosmotic flow(EOF) Protein Polypeptide |
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