苯二酚异构体的高效液相色谱化学发光检测法研究

高效液相色谱作为一种有效的分离手段已被用于酚类化合物的分析 ,紫外检测法 1～ 3] 、电化学检测法3,4 ] 等已用于苯二酚异构体的测定 ,但苯二酚异构体的化学发光检测法尚未见文献报道 .前文 5] 结果表明 ,苯二酚异构体等对鲁米诺诱导的化学发光具有很强的抑制作用 .基于此 ,本文将高效液相色谱与化学发光技术联用 ,首次建立了苯二酚异构体的高效液相色谱化学发光检测法 .该法灵敏度高 ,选择性好 ,为酚类物质的测定提供了一条新的途径 .1　实验部分1 .1　仪器与试剂　 FIA- 2 4 0 0型流动注射分析仪 (中国科学院信通科学仪器公司 ) ;G…

The Determination of Isomers of Dihydroxybenzenes by High Performance Liquid Chromatography with Chemiluminescent Detection

Based on the inhibition by hydroquinone, catechol and resorcinol of chemiluminescence from luminol potassium hexacyanoferrate(Ⅲ) system, a high performance liquid chromatographic procedure with chemiluminescenT_detection was developed for simultaneous determination of hydroquinone, catechol and resorcinol. The separation was carried out on RP-C₁₈ column at 37 ℃ by using a step gradient elution made by the mobile phases of methanol(A) and 0.05% H₃PO₄(B). The gradient elution condition was 95% B, 5 min; 95%B-50%B, 1 min; 50% B, 6 min. This method has the merits of high senstivity and good selectivity. The detection limits for hydroquinone, catechol and resorcinol are 2.0×10^-9 , 3.2×10^-9 and 6.8×10^-7 g/mL, respectively. The relative standard deviation( n =5) is 1.9% for 2.5×10^-7 g/mLhydroquinone, 1.0% for 7.5×10^-7 g/mLcatechol, 4.0% for 5.0×10^-5 g/mLresorcinol, respectively. The recovery is in the range of 94%-104%.