样条最小二乘法处理毛细管电泳数据的研究

毛细管电泳由于具有进样量少，分离效率高，分析速度快等优点而倍受关注［１～３］．目前商品化毛细电泳仪的检测器部分一般是光学敏感器，主要原因是光学检测受毛细管电泳的高压影响甚微，数据较为稳定，但是由于毛细管的内径很小，光程很短，大大限制了光学检测器的灵敏...

Studies on the Application of Spline Least Square Method to Capillary Electrophoresis

The Spline Least Square Method(SLSM) was applied to deal with the capillary electrophoresis graph containing a strong white nosie. The mathematical principle of SLSMwas introduced and the parameters, spline order k and filter length L, which affect the results directly, were discussed. Acapillary electrophoresis system with amperometric detector, micro Pt electrode as the working electrode, was demonstrated by running several phenols compounds in 50 μm×75 cm capillary. It shows SLSMhas a great applicability to handle complex graph with a strong white noise, and can give acceptable result even if the niose is as strong as the signal.