Multiplexed Determination of Fertility-related Hormones in Saliva Using Amperometric Immunosensing |
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Authors: | Beatriz Arévalo Verónica Serafín Susana Campuzano Paloma Yáñez-Sedeño José M Pingarrón |
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Institution: | Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, 28040- Madrid, Spain |
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Abstract: | This paper reports a dual immunosensor for the simultaneous determination of two important fertility-related hormones: 17β-estradiol, E2, and follicle-stimulating hormone, FSH. The implemented method involves direct competitive (E2) or sandwich-type (FSH) immunoassays carried out on magnetic microparticles (MBs) and amperometric detection at screen-printed dual carbon electrodes (SPdCEs) involving the hydroquinone (HQ)/H2O2 system. The developed immune platform demonstrates LOD values of 6.88 pg mL−1 and 0.11 mIU mL−1 for E2 and FSH standards, respectively and usefulness for the determination in saliva samples collected from different volunteers, giving results in agreement with the conventional ELISA methodologies. |
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Keywords: | Amperometry Immunosensing Fertility-related hormones Multiplexed Saliva |
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