|
|||||
|
|
| AE-HG-AFS测定长期汞暴露人群补硒后尿中硒的形态 | |
| 引用本文： | 黄笑寒,李玉锋,林婧,李柏,瞿丽雅,董泽琴,袁亚莉,高愈希,陈春英.AE-HG-AFS测定长期汞暴露人群补硒后尿中硒的形态[J].分析试验室,2012,31(1):11-15. |
| 作者姓名： | 黄笑寒 李玉锋 林婧 李柏 瞿丽雅 董泽琴 袁亚莉 高愈希 陈春英 |
| 作者单位： | 1. 中国科学院高能物理研究所多学科研究中心,北京100049;南华大学化学化工学院,衡阳421001 2. 中国科学院高能物理研究所多学科研究中心,北京,100049 3. 中国科学院高能物理研究所多学科研究中心,北京100049;福州大学食品安全分析与检测教育部重点实验室,福州350108 4. 贵州环境科学研究设计院,贵阳,550023 5. 南华大学化学化工学院,衡阳,421001 6. 中国科学院高能物理研究所多学科研究中心,北京100049;国家纳米科学中心中国科学院纳米生物效应与安全性重点实验室,北京100190 |
| 基金项目： | 中国科学院重要方向性，*，欧盟第六框架计划，贵州省科技厅社会发展攻关计划，中国科学院高能物理研究所青年基金项目资助 |
| 摘 要： | 建立了一种利用阴离子交换高效液相色谱与氢化物发生原子荧光光谱联用同时测定四、六价硒及硒代半胱氨酸(SeCys)形态的方法。优化了六价硒的还原条件及仪器检测参数,以不同浓度的柠檬酸铵作为流动相,在10 min内同时分离了四、六价硒及硒代半胱氨酸(SeCys)。采用加标法定量,加标回收率在90%～119%之间,相对标准偏差为1.6%～3.1%(100μg/L),四、六价硒及硒代半胱氨酸(SeCys)的检出限分别为0.32μg/L、0.47μg/L和0.44μg/L(进样量为100μL)。应用该法对长期汞暴露人群补硒后尿中的小分子硒的形态进行了分析,仅检测到硒代半胱氨酸(SeCys)。 |
| 关 键 词： | 阴离子交换色谱 氢化物发生原子荧光光谱 硒 形态 尿样 |
On-line analysis of selenium species in urine samples from mercury-exposed persons supplemented with selenium-enriched yeast by AE-HG-AFS |
|
| HUANG Xiao-han , LI Yu-feng , LIN Jing , LI Bai , QU Li-ya , DONG Ze-qin , YUAN Ya-li , GAO Yu-xi , CHEN Chun-ying.On-line analysis of selenium species in urine samples from mercury-exposed persons supplemented with selenium-enriched yeast by AE-HG-AFS[J].Chinese Journal of Analysis Laboratory,2012,31(1):11-15. | |
| Authors: | HUANG Xiao-han LI Yu-feng LIN Jing LI Bai QU Li-ya DONG Ze-qin YUAN Ya-li GAO Yu-xi CHEN Chun-ying |
| Institution: | 1,5(1.Multidiscipline Initiative Center,Institute of High Physics,Chinese Academy of Sciences,Beijing 100049;2.School of Chemistry and Chemical Engineering,University of South China,Hengyang 421001;3.Key Laboratory of Analysis and Detection Technology for Food Safety,Ministry of Education,Fuzhou university,Fuzhou 350108;4.Guizhou Institute of Environmental Science and Designing,Guiyang 550023;5.National Center for Nanoscience and Technology,Beijing 100190) |
| Abstract: | A method for simultaneously detecting species of Se(IV)、Se(VI) and SeCys was developed with anion exchange-hydride generation-atomic fluorescence spectrometry(AE-HG-AFS).Mobile phase A is composed of 0.5 mmol/L ammonium citrate(pH 3.7) while mobile phase B is 100 mmol/L ammonium citrate(pH 8.0).An anion exchange column(PRP-X100) with eluent of 85% A + 15% B was employed to separate these species within 10 min.The recoveries of spiked SeCys in urine samples were between 90% and 119% and the RSDs in five independent determinations varied from 1.6% to 3.1% at the concentration levels of 100μg/L.The detection limits for Se(IV),Se(VI) and SeCys were 0.32 μg/L,0.35 μg/L and 0.44 μg/L(100μL of injection),respectivety.This method was applied to analysis of urine samples from long-term mercury-exposed people supplemented with selenium-enriched yeast.We have only detected SeCys from the urine samples.The concentrations of total Se and SeCys in urine samples were found to increase after Se supplementation. |
| Keywords: | Anion exchange chromatography Hydride generation atomic fluorescence spectrometry Selenium Speciation Urine |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |