| 改良型单纯形法优化硝基喜树碱的毛细管电泳分离 |
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| 引用本文： | 黄河舟,柴逸峰,李吉学,赵亮,黄晟,乔善磊.改良型单纯形法优化硝基喜树碱的毛细管电泳分离[J].分析试验室,2004,23(9):29-32. |
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| 作者姓名： | 黄河舟 柴逸峰 李吉学 赵亮 黄晟 乔善磊 |
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| 作者单位： | 第二军医大学药学院,上海,200433;南京军医学院,南京,210049;东方肝胆医院药材科,上海,200433 |
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| 基金项目： | 上海市科技发展基金项目(01DJ19012) |
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| 摘 要： | 使用改良型单纯形法优化硝基喜树碱(Nitrocamprothecinum,NC)一对同分异构体的毛细管电泳分离。考察了运行电压、缓冲液的pH、硼砂和十二烷基磺酸钠(SDS)的浓度等4个影响因子,与传统的单因子优化方法及其结果进行比较。改良型单纯形法(采用CRS函数为响应函数)在分析方法的建立过程中显著减少了实验步骤,获得了更好的电泳分离条件。
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| 关 键 词： | 改良型单纯形法 优化 毛细管电泳 硝基喜树碱 同分异构体 分离 |
| 文章编号： | 1000-0720(2004)09-0029-04 |
Modified simplex optimization of nitrocamprothecinum separation by capillary electrophoresis |
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| HUANG He-zhou,CHAI Yi-feng,HUANG Sheng,QIAO Shan-lei.Modified simplex optimization of nitrocamprothecinum separation by capillary electrophoresis[J].Chinese Journal of Analysis Laboratory,2004,23(9):29-32. |
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| Authors: | HUANG He-zhou CHAI Yi-feng HUANG Sheng QIAO Shan-lei |
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| Abstract: | Modified simplex method was used in separation of nitrocamprothecinum (NC) isomers by capillary electrophoresis. The factors such as voltage, pH of buffer, borax concentration and SDS concentration in buffer were investigated. The optimization took much less steps than traditional single factor optimization. |
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| Keywords: | Simplex Optimization Capillary electrophoresis Nitrocamprothecinum Isomer Separation |
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