Production of biodiesel by immobilized Candida sp. lipase at high water content |
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Authors: | Tianwei Tan Kaili Nie Fang Wang |
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Institution: | (1) Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, 100029 Beijing, P.R. China |
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Abstract: | A new process for enzymatic synthesis of biodiesel at high water content (10–20%) with 96% conversion by lipase from Candida sp. 99–125 was studied. The lipase, a no-position-specific lipase, was immobilized by a cheap cotton membrane and the membrane-immobilized
lipase could be used at least six times with high conversion. The immobilized lipase could be used for different oil conversion
and preferred unsaturated fatty acids such as oleic acid to staturated fatty acids such as palmitic acid. The changes in concentration
of fatty acids, diglycerides, and methyl esters in the reaction were studied and a mechanism of synthesis of biodiesel was
suggested: the triglycerides are first enzymatically hydrolyzed into fatty acids, and then these fatty acids are further converted
into methyl esters. |
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Keywords: | Biodiesel lipase Candida sp lipase membrane immobilization |
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