Gas-phase enzymatic esterification on immobilized lipases in MCM-41 molecular sieves |
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Authors: | Pires Emerson Luis Miranda Everson Alves Valença Gustavo Paim |
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Institution: | 1.Department of Chemical Processes School of Chemical Engineering, State University of Campinas, PO Box 6066, 13081-970, Campains, SP, Brazil ;3.Department of Biotechnological Processes, School of Chemical Engineering, State University of Campinas, PO Box 6066, 13081-970, Campinas, SP, Brazil ; |
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Abstract: | Several lipolytic enzymes were immobilized in the pores of MCM-41 and Al-MCM-41 molecular sieves and used as catalysts in
the gas-phase esterification of acetic acid with ethanol. The entrapment of enzymes depended on the molecular sieve and the
type of enzyme used. The order of enzymatic activity for enzymes entrapped in the pores of MCM-41 and Al-MCM-41 in the esterification
reaction was OF (Rhizopus niveus lipases)<FAP-15 (Rhizopus oryzae lipases)<LEX (Mucor javanicus lipases)<PS (Pseudomonas cepacia lipases)<AK (Pseudomonas fluorescens lipases). Experiments carried out between 298 and 318 K showed no effect of temperature on catalyst yield, suggesting that
the enzymes were appropriately immobilized in the pores of the molecular sieves, thus avoiding possible processes such as
denaturing or autolysis. |
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Keywords: | Enzyme lipase molecular sieves MCM-41 ethyl acetate gas-phase |
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