氨基酸-磷酸非共价复合物离子的电喷雾电离质谱研究

分子间的非共价相互作用对于理解分子复合物的结构和性质有着非常重要的意义。而电喷雾电离质谱(ESI-MS)是团簇化学研究的一种重要工具,广泛用于重要生物分子复合物的形成和性质研究,包括氨基酸、多肽和核糖等。该文将一定化学剂量比的磷酸分别与L型精氨酸和L型色氨酸混合,通过电喷雾电离的方法,利用傅立叶变换离子回旋共振质谱仪对精氨酸-磷酸以及色氨酸-磷酸的非共价复合物离子进行了研究。结果显示,精氨酸和色氨酸均可与磷酸分子产生种类丰富、价态为+1和+2的复合物离子。而对不同体系的生成离子分布进行分析,则显示出不同体系间的差别。

Investigation of Noncovalent Complexions of Amino Acids and Phosphoric Acid by Electrospray Mass Spectrometry

Intermolecular noncovalent interaction is very important for understanding structures and properties of molecular complexes and clusters.Electrospray ionization mass spectrometry(ESI-MS)has demonstrated its powerful utility in the detection and research of weakly bound complex ions,including amino acids,peptides and proteins.In this paper,the noncovalent complex ions of arginine and tryptophan with phosphoric acid were investigated by ESI-MS method.The experiments were performed on a Fourier transform ion cyclotron resonance (FT ICR) mass spectrometer.The results showed that for both systems,wide varieties of noncovalent complex ions were generated and detected in the mass spectra.Most of these complex cations are singly or doubly charged,including both units of amino acid and phosphoric acid.The differences among different systems are also reflected by the distributions of the formed complex ions.