聚多巴胺辅助的液相沉积法制备二氧化钛涂层毛细管柱及其在电色谱中的应用

利用多巴胺的自氧化聚合反应在毛细管内壁引入聚多巴胺涂层,并以聚多巴胺涂层作为连接臂辅助二氧化钛前躯体氟钛酸铵液相沉积制备了二氧化钛涂层毛细管柱。该方法制备过程简单,条件温和,形成的涂层稳固。采用X射线光电子能谱(XPS)、扫描电镜(SEM)和测定电渗流变化对涂层性质进行了表征。选择5种阴离子、生物碱作为分离对象,考察了缓冲液组成、浓度和p H值等因素对该涂层柱毛细管电色谱分离性能的影响。结果显示,在优化条件下,5种阴离子及生物碱在该涂层毛细管柱上均能得到较好的分离。

Preparation of TiO2 Coated Column Capillary by Polydopamine Assisted Liquid Phase Deposition and Its Application in Capillary Electrochromatography

The polydopamine,formed by spontaneous oxidative polymerization of dopamine,was introduced onto the inner surface of capillary,and then exploited as an adhesive coating to prepare TiO2 coated capillary column by assisting liquid phase deposition process of ammonium fluotitanate which was used as precursor of titanium dioxide.The method had the advantages of facile preparation process,operation under mild reaction conditions and the formation of a stable coating.The titanium dioxide coating was characterized by X ray photoelectron spectroscopy(XPS) and scanning electron microscope(SEM).A comparative study of electroosmotic flow(EOF) was also investigated.Five common inorganic anions and three alkaloids were selected as the test analytes.Effects of several factors,such as composition of background electrolytes(BGEs),buffer concentration and pH value,on separation performance were investigated.The results showed that,under the optimal conditions,both five inorganic anions and three alkaloids could be well separated.