重金属铬离子胶体金免疫层析快速检测法的建立

建立了水样中铬离子(Cr3+)检测的免疫试纸条检测方法。将自制的抗Cr3+-EDTA单克隆抗体标记于金颗粒后,包被于玻璃纤维上,将Cr3+-EDTA-BSA抗原和羊抗鼠IgG二抗分别结合于硝酸纤维膜上作为检测线(T线)和质控线(C线),组装试纸条。该试纸条可检测经亚硫酸氢钠还原为Cr3+的Cr6+,检出限为50μg/L;与10种重金属进行交叉实验验证,除与1 000μg/L的Fe3+有微弱交叉外,与其他重金属均无交叉反应;比较试纸条与仪器法ICP检测加标水样,两种方法的结果呈正相关。该试纸条可在3～5 min内肉眼观察结果,适用于现场检测,满足水样或土壤中重金属铬残留的监测要求。

Rapid Detection of Chromium Ion by Gold Immunochromatographic Assay

An immunochromatographic assay(ICA) method for the detection of chromium ions(Cr3+) was developed.Colloidal gold particles coated with affinity purified monoclonal anti-isothiocyanobenzyl-EDTA(iEDAT)-chelated Cr3+ antibodies were used as detection reagent,and the strip was prepared by arraying the synthetic Cr3+-EDTA-BSA and goat anti-mouse immunoglobulins on cellulose nitrate film to form the test(T) band and control(C) band.The prepared strip was used to detect Cr6+ when it was reduced to Cr3+ by NaHSO3.The visual lowest detection limities(LDL) of the test strip was as low as 50 μg/L.The test trips showed negligible cross-reactivities with 10 heavy metals except for 1 000 μg/L Fe3+-EDTA.The determination result of water samples containing chromium standard by the prepared strip was consistent with that by ICP.Because of its sensitivity,specifity,low cost and fast result(within 5 min),the test strip is especially suitable for the on-site large scale screening on Cr-contained water samples and many other applications.