Recent developments in optical detection methods for microchip separations |
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Authors: | Sebastian Götz Uwe Karst |
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Institution: | (1) Chemical Analysis Group and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands;(2) Present address: Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany |
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Abstract: | This paper summarizes the features and performances of optical detection systems currently applied in order to monitor separations
on microchip devices. Fluorescence detection, which delivers very high sensitivity and selectivity, is still the most widely
applied method of detection. Instruments utilizing laser-induced fluorescence (LIF) and lamp-based fluorescence along with
recent applications of light-emitting diodes (LED) as excitation sources are also covered in this paper. Since chemiluminescence
detection can be achieved using extremely simple devices which no longer require light sources and optical components for
focusing and collimation, interesting approaches based on this technique are presented, too. Although UV/vis absorbance is
a detection method that is commonly used in standard desktop electrophoresis and liquid chromatography instruments, it has
not yet reached the same level of popularity for microchip applications. Current applications of UV/vis absorbance detection
to microchip separations and innovative approaches that increase sensitivity are described. This article, which contains 85
references, focuses on developments and applications published within the last three years, points out exciting new approaches,
and provides future perspectives on this field. |
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Keywords: | Optical detection methods Microchip Microfluidic Separation Review |
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