Prostate-specific antigen immunoassays on the BioCD |
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Authors: | Xuefeng Wang Ming Zhao David D Nolte |
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Institution: | (1) Department of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907-2036, USA |
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Abstract: | The BioCD is a spinning-disc interferometric biosensor on which antibodies are immobilized to capture target antigens from
biological samples. In this work, BioCDs measured the interferometric response to prostate-specific antigen (PSA). The ideal
detection limit for PSA was determined using a BioCD with 12,500 printed target antibody spots with a corresponding number
of reference protein spots. Statistical analysis projects the detection limit of PSA as a function of the number of spots
included in the average. When approximately 10,000 spot pairs were averaged, the 3σ detection limit was 60 pg/ml in a 2 mg/ml simple protein background. A standard format for BioCD immunoassays uses 96 wells
with 32 target spots paired with reference spots. In serum, the detection limit for this format was 1 ng/ml in 3:1 diluted
female human serum using a sandwich assay with a nonfluorescent mass tag. |
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Keywords: | Optical biosensor BioCD Label-free Prostate cancer Prostate-specific antigen Antibody microarray |
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