毛细管电泳扫描伏安电化学法分离检测肾上腺素、氯丙嗪和异丙嗪

采用自制的高效毛细管电泳扫描伏安电化学检测装置，在磷酸盐缓冲溶液中进行了肾上腺素、异丙嗪和氯丙嗪的分离检测，在优化的条件下获得了较好的检测重现性和低的检出限。结果表明，采用扫描伏安电化学方法不但能够减少电极的污染，而且可以在电泳过程中获得被测物质的伏安特性及检测体系的动态伏安图，更有利于被测物质的识别。

Determination of Epinephrine, Chlorpromazine and Promethazine by Capillary Electrophoresis with Scanning Voltammetric Detector

With a self-constructed electrochemical detection system, the determination of epinephrine, chlorpromazine and promethazine has been studied by using capillary electrophoresis with scanning voltammetric detector. Scanning potential generation and data acquisition were carried out with D/A and A/D converter board that mounted on a 486DX66 computer. A complex software, which was written by authors in BORLAND C++, was serve to data acquisition, abstraction of three-dimension data, display and print of the electrophorograms. Using a 25 microns x 70 cm fused-silica capillary, the optimum conditions for separation and detection of titled drugs are 10 mmol/L K2HPO4 + H3PO4 (pH 3.0) running electrolyte, 20 kV separation voltage, hydrodynamic injection 10 s at 20 cm height, scanning potential from +0.4 to +1.0 V vs. SCE. With the optimizing condition, the separation of titled drugs is satisfactory, and good reproducibility and low limit are obtained. In this study, all experiments are carried out under air-conditioning (18 degrees C) and constant humidity (relative humidity 50%). In comparison to amperometric detection, scanning voltammetric detection has the advantages of minimizing fouling of the working electrode surface, and direct identification of unknown chemicals by voltammetric curves.