三种维生素的毛细管电泳和胶束电动毛细管色谱法分离与安培电化学检测

以自制毛细管电泳－电化学检测系统对ＶＣ，ＶＢ１和ＶＢ６的毛细管区带电泳和胶束电动色谱的分离与检测情况进行了初探。结果表明，在０．０１ｍｏｌ／ＬＮＨ３－ＮＨ４Ｃｌ介质中，检测电势定于５１０～５４０ｍＶ（对ＳＣＥ）时，三种维生素均有较好的ＣＺＥ图，对ＶＣ的分离效率达４６８８００块理论板。使用十二烷基硫酸钠时，分离效果欠佳。

Preliminary Study on Separation of Three Vitamins by Capillary Zone Electrophoresis and Micellar Electrokinetic Chromatography with Amperometric Electrochemical Detection

In this paper, separation of water soluble vitamins C (VC), B1 (VB1) and B6 (VB6) has been studied preliminarily by capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MECC) with a home-made amperometric electrochemical detector at the working electrode carbon fiber (5 microm o.d.)] potential of 510-540 mV (vs. SCE). It has been shown that each of the three vitamins has more than one peak in NH3-NH4Cl (pH 9.0) which were mainly caused by the decomposition products in weak base medium and by heat except those from impurities. The electrochemical detector has better performance for the three vitamins from CZE and MECC. They can be separated either by CZE or by MECC. The separation by CZE is better than that by MECC with sodium dodecyl sulfate (SDS). SDS and buffer acidity can influence the retention time, peak sequence and height. The separation efficiency of CZE in 0.01 mol/L NH3-NH4Cl was 468 800 theoretical plates for VC.